Architecture of thermal adaptation in an Exiguobacterium sibiricumstrain isolated from 3 million year old permafrost: A genome and transcriptome approach
Abstract Background Many microorganisms have a wide temperature growth range and versatility to tolerate large thermal fluctuations in diverse environments, however not many have been fully explored over their entire growth temperature range through a holistic view of its physiology, genome, and tra...
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ftbiomed:oai:biomedcentral.com:1471-2164-9-547 2023-05-15T17:57:30+02:00 Architecture of thermal adaptation in an Exiguobacterium sibiricumstrain isolated from 3 million year old permafrost: A genome and transcriptome approach Rodrigues, Debora F Ivanova, Natalia He, Zhili Huebner, Marianne Zhou, Jizhong Tiedje, James M 2008-11-18 http://www.biomedcentral.com/1471-2164/9/547 en eng BioMed Central Ltd. http://www.biomedcentral.com/1471-2164/9/547 Copyright 2008 Rodrigues et al; licensee BioMed Central Ltd. Research article 2008 ftbiomed 2009-01-24T00:13:48Z Abstract Background Many microorganisms have a wide temperature growth range and versatility to tolerate large thermal fluctuations in diverse environments, however not many have been fully explored over their entire growth temperature range through a holistic view of its physiology, genome, and transcriptome. We used Exiguobacterium sibiricum strain 255-15, a psychrotrophic bacterium from 3 million year old Siberian permafrost that grows from -5°C to 39°C to study its thermal adaptation. Results The E. sibiricum genome has one chromosome and two small plasmids with a total of 3,015 protein-encoding genes (CDS), and a GC content of 47.7%. The genome and transcriptome analysis along with the organism's known physiology was used to better understand its thermal adaptation. A total of 27%, 3.2%, and 5.2% of E. sibiricum CDS spotted on the DNA microarray detected differentially expressed genes in cells grown at -2.5°C, 10°C, and 39°C, respectively, when compared to cells grown at 28°C. The hypothetical and unknown genes represented 10.6%, 0.89%, and 2.3% of the CDS differentially expressed when grown at -2.5°C, 10°C, and 39°C versus 28°C, respectively. Conclusion The results show that E. sibiricum is constitutively adapted to cold temperatures stressful to mesophiles since little differential gene expression was observed between 4°C and 28°C, but at the extremities of its Arrhenius growth profile, namely -2.5°C and 39°C, several physiological and metabolic adaptations associated with stress responses were observed. Article in Journal/Newspaper permafrost BioMed Central |
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Abstract Background Many microorganisms have a wide temperature growth range and versatility to tolerate large thermal fluctuations in diverse environments, however not many have been fully explored over their entire growth temperature range through a holistic view of its physiology, genome, and transcriptome. We used Exiguobacterium sibiricum strain 255-15, a psychrotrophic bacterium from 3 million year old Siberian permafrost that grows from -5°C to 39°C to study its thermal adaptation. Results The E. sibiricum genome has one chromosome and two small plasmids with a total of 3,015 protein-encoding genes (CDS), and a GC content of 47.7%. The genome and transcriptome analysis along with the organism's known physiology was used to better understand its thermal adaptation. A total of 27%, 3.2%, and 5.2% of E. sibiricum CDS spotted on the DNA microarray detected differentially expressed genes in cells grown at -2.5°C, 10°C, and 39°C, respectively, when compared to cells grown at 28°C. The hypothetical and unknown genes represented 10.6%, 0.89%, and 2.3% of the CDS differentially expressed when grown at -2.5°C, 10°C, and 39°C versus 28°C, respectively. Conclusion The results show that E. sibiricum is constitutively adapted to cold temperatures stressful to mesophiles since little differential gene expression was observed between 4°C and 28°C, but at the extremities of its Arrhenius growth profile, namely -2.5°C and 39°C, several physiological and metabolic adaptations associated with stress responses were observed. |
format |
Article in Journal/Newspaper |
author |
Rodrigues, Debora F Ivanova, Natalia He, Zhili Huebner, Marianne Zhou, Jizhong Tiedje, James M |
spellingShingle |
Rodrigues, Debora F Ivanova, Natalia He, Zhili Huebner, Marianne Zhou, Jizhong Tiedje, James M Architecture of thermal adaptation in an Exiguobacterium sibiricumstrain isolated from 3 million year old permafrost: A genome and transcriptome approach |
author_facet |
Rodrigues, Debora F Ivanova, Natalia He, Zhili Huebner, Marianne Zhou, Jizhong Tiedje, James M |
author_sort |
Rodrigues, Debora F |
title |
Architecture of thermal adaptation in an Exiguobacterium sibiricumstrain isolated from 3 million year old permafrost: A genome and transcriptome approach |
title_short |
Architecture of thermal adaptation in an Exiguobacterium sibiricumstrain isolated from 3 million year old permafrost: A genome and transcriptome approach |
title_full |
Architecture of thermal adaptation in an Exiguobacterium sibiricumstrain isolated from 3 million year old permafrost: A genome and transcriptome approach |
title_fullStr |
Architecture of thermal adaptation in an Exiguobacterium sibiricumstrain isolated from 3 million year old permafrost: A genome and transcriptome approach |
title_full_unstemmed |
Architecture of thermal adaptation in an Exiguobacterium sibiricumstrain isolated from 3 million year old permafrost: A genome and transcriptome approach |
title_sort |
architecture of thermal adaptation in an exiguobacterium sibiricumstrain isolated from 3 million year old permafrost: a genome and transcriptome approach |
publisher |
BioMed Central Ltd. |
publishDate |
2008 |
url |
http://www.biomedcentral.com/1471-2164/9/547 |
genre |
permafrost |
genre_facet |
permafrost |
op_relation |
http://www.biomedcentral.com/1471-2164/9/547 |
op_rights |
Copyright 2008 Rodrigues et al; licensee BioMed Central Ltd. |
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1766165943727161344 |