Inflammatory responses in primary muscle cell cultures in Atlantic salmon (Salmo salar)
Abstract Background The relationship between fish health and muscle growth is critical for continued expansion of the aquaculture industry. The effect of immune stimulation on the expression of genes related to the energy balance of fish is poorly understood. In mammals immune stimulation results in...
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ftbiomed:oai:biomedcentral.com:1471-2164-14-747 2023-05-15T15:30:31+02:00 Inflammatory responses in primary muscle cell cultures in Atlantic salmon (Salmo salar) Pooley, Nicholas J Tacchi, Luca Secombes, Christopher J Martin, Samuel AM 2013-11-01 http://www.biomedcentral.com/1471-2164/14/747 en eng BioMed Central Ltd. http://www.biomedcentral.com/1471-2164/14/747 Copyright 2013 Pooley et al.; licensee BioMed Central Ltd. Transcriptomics Atlantic salmon (Salmo salar) Muscle cell culture Inflammation Catabolism Cell cycle IGF binding proteins Research article 2013 ftbiomed 2013-11-10T01:22:18Z Abstract Background The relationship between fish health and muscle growth is critical for continued expansion of the aquaculture industry. The effect of immune stimulation on the expression of genes related to the energy balance of fish is poorly understood. In mammals immune stimulation results in major transcriptional changes in muscle, potentially to allow a reallocation of amino acids for use in the immune response and energy homeostasis. The aim of this study was to investigate the effects of immune stimulation on fish muscle gene expression. Results Atlantic salmon ( Salmo salar ) primary muscle cell cultures were stimulated with recombinant (r)IL-1β, a major proinflammatory cytokine, for 24 h in order to simulate an acute immune response. The transcriptomic response was determined by RNA hybridization to a 4 × 44 K Agilent Atlantic salmon microarray platform. The rIL-1β stimulation induced the expression of genes related to both the innate and adaptive immune systems. In addition there were highly significant changes in the expression of genes related to regulation of the cell cycle, growth/structural proteins, proteolysis and lipid metabolism. Of interest were a number of IGF binding proteins that were differentially expressed, which may demonstrate cross talk between the growth and immune systems. Conclusion We show rIL-1β modulates the expression of not only immune related genes, but also that of genes involved in processes related to growth and metabolism. Co-stimulation of muscle cells with both rIGF-I and rIL-1β demonstrates cross talk between these pathways providing potential avenues for further research. This study highlights the potential negative effects of inflammation on muscle protein deposition and growth in fish and extends our understanding of energy allocation in ectothermic animals. Article in Journal/Newspaper Atlantic salmon Salmo salar BioMed Central |
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BioMed Central |
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English |
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Transcriptomics Atlantic salmon (Salmo salar) Muscle cell culture Inflammation Catabolism Cell cycle IGF binding proteins |
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Transcriptomics Atlantic salmon (Salmo salar) Muscle cell culture Inflammation Catabolism Cell cycle IGF binding proteins Pooley, Nicholas J Tacchi, Luca Secombes, Christopher J Martin, Samuel AM Inflammatory responses in primary muscle cell cultures in Atlantic salmon (Salmo salar) |
topic_facet |
Transcriptomics Atlantic salmon (Salmo salar) Muscle cell culture Inflammation Catabolism Cell cycle IGF binding proteins |
description |
Abstract Background The relationship between fish health and muscle growth is critical for continued expansion of the aquaculture industry. The effect of immune stimulation on the expression of genes related to the energy balance of fish is poorly understood. In mammals immune stimulation results in major transcriptional changes in muscle, potentially to allow a reallocation of amino acids for use in the immune response and energy homeostasis. The aim of this study was to investigate the effects of immune stimulation on fish muscle gene expression. Results Atlantic salmon ( Salmo salar ) primary muscle cell cultures were stimulated with recombinant (r)IL-1β, a major proinflammatory cytokine, for 24 h in order to simulate an acute immune response. The transcriptomic response was determined by RNA hybridization to a 4 × 44 K Agilent Atlantic salmon microarray platform. The rIL-1β stimulation induced the expression of genes related to both the innate and adaptive immune systems. In addition there were highly significant changes in the expression of genes related to regulation of the cell cycle, growth/structural proteins, proteolysis and lipid metabolism. Of interest were a number of IGF binding proteins that were differentially expressed, which may demonstrate cross talk between the growth and immune systems. Conclusion We show rIL-1β modulates the expression of not only immune related genes, but also that of genes involved in processes related to growth and metabolism. Co-stimulation of muscle cells with both rIGF-I and rIL-1β demonstrates cross talk between these pathways providing potential avenues for further research. This study highlights the potential negative effects of inflammation on muscle protein deposition and growth in fish and extends our understanding of energy allocation in ectothermic animals. |
format |
Article in Journal/Newspaper |
author |
Pooley, Nicholas J Tacchi, Luca Secombes, Christopher J Martin, Samuel AM |
author_facet |
Pooley, Nicholas J Tacchi, Luca Secombes, Christopher J Martin, Samuel AM |
author_sort |
Pooley, Nicholas J |
title |
Inflammatory responses in primary muscle cell cultures in Atlantic salmon (Salmo salar) |
title_short |
Inflammatory responses in primary muscle cell cultures in Atlantic salmon (Salmo salar) |
title_full |
Inflammatory responses in primary muscle cell cultures in Atlantic salmon (Salmo salar) |
title_fullStr |
Inflammatory responses in primary muscle cell cultures in Atlantic salmon (Salmo salar) |
title_full_unstemmed |
Inflammatory responses in primary muscle cell cultures in Atlantic salmon (Salmo salar) |
title_sort |
inflammatory responses in primary muscle cell cultures in atlantic salmon (salmo salar) |
publisher |
BioMed Central Ltd. |
publishDate |
2013 |
url |
http://www.biomedcentral.com/1471-2164/14/747 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
http://www.biomedcentral.com/1471-2164/14/747 |
op_rights |
Copyright 2013 Pooley et al.; licensee BioMed Central Ltd. |
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