Physiological change under OsHV-1 contamination in Pacific oyster Crassostrea gigas through massive mortality events on fields
Abstract Background Massive mortalities have been observed in France since 2008 on spat and juvenile Pacific oysters, Crassostrea gigas . A herpes virus called OsHV-1, easily detectable by PCR, has been implicated in the mortalities as demonstrated by the results of numerous field studies linking mo...
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ftbiomed:oai:biomedcentral.com:1471-2164-14-590 2023-05-15T15:57:44+02:00 Physiological change under OsHV-1 contamination in Pacific oyster Crassostrea gigas through massive mortality events on fields Jouaux, Aude Lafont, Maxime Blin, Jean-Louis Houssin, Maryline Mathieu, Michel Lelong, Christophe 2013-08-29 http://www.biomedcentral.com/1471-2164/14/590 en eng BioMed Central Ltd. http://www.biomedcentral.com/1471-2164/14/590 Copyright 2013 Jouaux et al.; licensee BioMed Central Ltd. Crassostrea gigas OsHV-1 Host response Mortality Transcriptome Research article 2013 ftbiomed 2013-09-15T04:42:55Z Abstract Background Massive mortalities have been observed in France since 2008 on spat and juvenile Pacific oysters, Crassostrea gigas . A herpes virus called OsHV-1, easily detectable by PCR, has been implicated in the mortalities as demonstrated by the results of numerous field studies linking mortality with OsHV-1 prevalence. Moreover, experimental infections using viral particles have documented the pathogenicity of OsHV-1 but the physiological responses of host to pathogen are not well known. Results The aim of this study was to understand mechanisms brought into play against the virus during infection in the field. A microarray assay has been developed for a major part of the oyster genome and used for studying the host transcriptome across mortality on field. Spat with and without detectable OsHV-1 infection presenting or not mortality respectively were compared by microarray during mortality episodes. In this study, a number of genes are regulated in the response to pathogen infection on field and seems to argue to an implication of the virus in the observed mortality. The result allowed establishment of a hypothetic scheme of the host cell’s infection by, and response to, the pathogen. Conclusions This response shows a “sensu stricto” innate immunity through genic regulation of the virus OsHV-1 life cycle, but also others biological processes resulting to complex interactions between host and pathogens in general. Article in Journal/Newspaper Crassostrea gigas Pacific oyster BioMed Central Pacific |
institution |
Open Polar |
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BioMed Central |
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ftbiomed |
language |
English |
topic |
Crassostrea gigas OsHV-1 Host response Mortality Transcriptome |
spellingShingle |
Crassostrea gigas OsHV-1 Host response Mortality Transcriptome Jouaux, Aude Lafont, Maxime Blin, Jean-Louis Houssin, Maryline Mathieu, Michel Lelong, Christophe Physiological change under OsHV-1 contamination in Pacific oyster Crassostrea gigas through massive mortality events on fields |
topic_facet |
Crassostrea gigas OsHV-1 Host response Mortality Transcriptome |
description |
Abstract Background Massive mortalities have been observed in France since 2008 on spat and juvenile Pacific oysters, Crassostrea gigas . A herpes virus called OsHV-1, easily detectable by PCR, has been implicated in the mortalities as demonstrated by the results of numerous field studies linking mortality with OsHV-1 prevalence. Moreover, experimental infections using viral particles have documented the pathogenicity of OsHV-1 but the physiological responses of host to pathogen are not well known. Results The aim of this study was to understand mechanisms brought into play against the virus during infection in the field. A microarray assay has been developed for a major part of the oyster genome and used for studying the host transcriptome across mortality on field. Spat with and without detectable OsHV-1 infection presenting or not mortality respectively were compared by microarray during mortality episodes. In this study, a number of genes are regulated in the response to pathogen infection on field and seems to argue to an implication of the virus in the observed mortality. The result allowed establishment of a hypothetic scheme of the host cell’s infection by, and response to, the pathogen. Conclusions This response shows a “sensu stricto” innate immunity through genic regulation of the virus OsHV-1 life cycle, but also others biological processes resulting to complex interactions between host and pathogens in general. |
format |
Article in Journal/Newspaper |
author |
Jouaux, Aude Lafont, Maxime Blin, Jean-Louis Houssin, Maryline Mathieu, Michel Lelong, Christophe |
author_facet |
Jouaux, Aude Lafont, Maxime Blin, Jean-Louis Houssin, Maryline Mathieu, Michel Lelong, Christophe |
author_sort |
Jouaux, Aude |
title |
Physiological change under OsHV-1 contamination in Pacific oyster Crassostrea gigas through massive mortality events on fields |
title_short |
Physiological change under OsHV-1 contamination in Pacific oyster Crassostrea gigas through massive mortality events on fields |
title_full |
Physiological change under OsHV-1 contamination in Pacific oyster Crassostrea gigas through massive mortality events on fields |
title_fullStr |
Physiological change under OsHV-1 contamination in Pacific oyster Crassostrea gigas through massive mortality events on fields |
title_full_unstemmed |
Physiological change under OsHV-1 contamination in Pacific oyster Crassostrea gigas through massive mortality events on fields |
title_sort |
physiological change under oshv-1 contamination in pacific oyster crassostrea gigas through massive mortality events on fields |
publisher |
BioMed Central Ltd. |
publishDate |
2013 |
url |
http://www.biomedcentral.com/1471-2164/14/590 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_relation |
http://www.biomedcentral.com/1471-2164/14/590 |
op_rights |
Copyright 2013 Jouaux et al.; licensee BioMed Central Ltd. |
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