Surviving in a toxic world: transcriptomics and gene expression profiling in response to environmental pollution in the critically endangered European eel
Abstract Background Genomic and transcriptomic approaches have the potential for unveiling the genome-wide response to environmental perturbations. The abundance of the catadromous European eel ( Anguilla anguilla ) stock has been declining since the 1980s probably due to a combination of anthropoge...
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ftbiomed:oai:biomedcentral.com:1471-2164-13-507 2023-05-15T13:27:18+02:00 Surviving in a toxic world: transcriptomics and gene expression profiling in response to environmental pollution in the critically endangered European eel Pujolar, Jose Marino, Ilaria AM Milan, Massimo Coppe, Alessandro Maes, Gregory E Capoccioni, Fabrizio Ciccotti, Eleonora Bervoets, Lieven Covaci, Adrian Belpaire, Claude Cramb, Gordon Patarnello, Tomaso Bargelloni, Luca Bortoluzzi, Stefania Zane, Lorenzo 2012-09-25 http://www.biomedcentral.com/1471-2164/13/507 en eng BioMed Central Ltd. http://www.biomedcentral.com/1471-2164/13/507 Copyright 2012 Pujolar et al.; licensee BioMed Central Ltd. Anguilla anguilla European eel Transcriptome Microarrays Pollution Research article 2012 ftbiomed 2012-12-30T01:00:33Z Abstract Background Genomic and transcriptomic approaches have the potential for unveiling the genome-wide response to environmental perturbations. The abundance of the catadromous European eel ( Anguilla anguilla ) stock has been declining since the 1980s probably due to a combination of anthropogenic and climatic factors. In this paper, we explore the transcriptomic dynamics between individuals from high (river Tiber, Italy) and low pollution (lake Bolsena, Italy) environments, which were measured for 36 PCBs, several organochlorine pesticides and brominated flame retardants and nine metals. Results To this end, we first (i) updated the European eel transcriptome using deep sequencing data with a total of 640,040 reads assembled into 44,896 contigs (Eeelbase release 2.0), and (ii) developed a transcriptomic platform for global gene expression profiling in the critically endangered European eel of about 15,000 annotated contigs, which was applied to detect differentially expressed genes between polluted sites. Several detoxification genes related to metabolism of pollutants were upregulated in the highly polluted site, including genes that take part in phase I of the xenobiotic metabolism (CYP3A), phase II (glutathione-S-transferase) and oxidative stress (glutathione peroxidase). In addition, key genes in the mitochondrial respiratory chain and oxidative phosphorylation were down-regulated at the Tiber site relative to the Bolsena site. Conclusions Together with the induced high expression of detoxification genes, the suggested lowered expression of genes supposedly involved in metabolism suggests that pollution may also be associated with decreased respiratory and energy production. Article in Journal/Newspaper Anguilla anguilla BioMed Central Tiber ENVELOPE(-66.983,-66.983,-68.392,-68.392) |
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BioMed Central |
op_collection_id |
ftbiomed |
language |
English |
topic |
Anguilla anguilla European eel Transcriptome Microarrays Pollution |
spellingShingle |
Anguilla anguilla European eel Transcriptome Microarrays Pollution Pujolar, Jose Marino, Ilaria AM Milan, Massimo Coppe, Alessandro Maes, Gregory E Capoccioni, Fabrizio Ciccotti, Eleonora Bervoets, Lieven Covaci, Adrian Belpaire, Claude Cramb, Gordon Patarnello, Tomaso Bargelloni, Luca Bortoluzzi, Stefania Zane, Lorenzo Surviving in a toxic world: transcriptomics and gene expression profiling in response to environmental pollution in the critically endangered European eel |
topic_facet |
Anguilla anguilla European eel Transcriptome Microarrays Pollution |
description |
Abstract Background Genomic and transcriptomic approaches have the potential for unveiling the genome-wide response to environmental perturbations. The abundance of the catadromous European eel ( Anguilla anguilla ) stock has been declining since the 1980s probably due to a combination of anthropogenic and climatic factors. In this paper, we explore the transcriptomic dynamics between individuals from high (river Tiber, Italy) and low pollution (lake Bolsena, Italy) environments, which were measured for 36 PCBs, several organochlorine pesticides and brominated flame retardants and nine metals. Results To this end, we first (i) updated the European eel transcriptome using deep sequencing data with a total of 640,040 reads assembled into 44,896 contigs (Eeelbase release 2.0), and (ii) developed a transcriptomic platform for global gene expression profiling in the critically endangered European eel of about 15,000 annotated contigs, which was applied to detect differentially expressed genes between polluted sites. Several detoxification genes related to metabolism of pollutants were upregulated in the highly polluted site, including genes that take part in phase I of the xenobiotic metabolism (CYP3A), phase II (glutathione-S-transferase) and oxidative stress (glutathione peroxidase). In addition, key genes in the mitochondrial respiratory chain and oxidative phosphorylation were down-regulated at the Tiber site relative to the Bolsena site. Conclusions Together with the induced high expression of detoxification genes, the suggested lowered expression of genes supposedly involved in metabolism suggests that pollution may also be associated with decreased respiratory and energy production. |
format |
Article in Journal/Newspaper |
author |
Pujolar, Jose Marino, Ilaria AM Milan, Massimo Coppe, Alessandro Maes, Gregory E Capoccioni, Fabrizio Ciccotti, Eleonora Bervoets, Lieven Covaci, Adrian Belpaire, Claude Cramb, Gordon Patarnello, Tomaso Bargelloni, Luca Bortoluzzi, Stefania Zane, Lorenzo |
author_facet |
Pujolar, Jose Marino, Ilaria AM Milan, Massimo Coppe, Alessandro Maes, Gregory E Capoccioni, Fabrizio Ciccotti, Eleonora Bervoets, Lieven Covaci, Adrian Belpaire, Claude Cramb, Gordon Patarnello, Tomaso Bargelloni, Luca Bortoluzzi, Stefania Zane, Lorenzo |
author_sort |
Pujolar, Jose |
title |
Surviving in a toxic world: transcriptomics and gene expression profiling in response to environmental pollution in the critically endangered European eel |
title_short |
Surviving in a toxic world: transcriptomics and gene expression profiling in response to environmental pollution in the critically endangered European eel |
title_full |
Surviving in a toxic world: transcriptomics and gene expression profiling in response to environmental pollution in the critically endangered European eel |
title_fullStr |
Surviving in a toxic world: transcriptomics and gene expression profiling in response to environmental pollution in the critically endangered European eel |
title_full_unstemmed |
Surviving in a toxic world: transcriptomics and gene expression profiling in response to environmental pollution in the critically endangered European eel |
title_sort |
surviving in a toxic world: transcriptomics and gene expression profiling in response to environmental pollution in the critically endangered european eel |
publisher |
BioMed Central Ltd. |
publishDate |
2012 |
url |
http://www.biomedcentral.com/1471-2164/13/507 |
long_lat |
ENVELOPE(-66.983,-66.983,-68.392,-68.392) |
geographic |
Tiber |
geographic_facet |
Tiber |
genre |
Anguilla anguilla |
genre_facet |
Anguilla anguilla |
op_relation |
http://www.biomedcentral.com/1471-2164/13/507 |
op_rights |
Copyright 2012 Pujolar et al.; licensee BioMed Central Ltd. |
_version_ |
1766397642684760064 |