The dermomyotome ventrolateral lip is essential for the hypaxial myotome formation
Abstract Background The myotome is the primitive skeletal muscle that forms within the embryonic metameric body wall. It can be subdivided into an epaxial and hypaxial domain. It has been shown that the formation of the epaxial myotome requires the dorsomedial lip of the dermomyotome (DML). Although...
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ftbiomed:oai:biomedcentral.com:1471-213X-13-37 2023-05-15T16:02:06+02:00 The dermomyotome ventrolateral lip is essential for the hypaxial myotome formation Pu, Qin Abduelmula, Aisha Masyuk, Maryna Theiss, Carsten Schwandulla, Dieter Hans, Michael Patel, Ketan Brand-Saberi, Beate Huang, Ruijin 2013-10-18 http://www.biomedcentral.com/1471-213X/13/37 en eng BioMed Central Ltd. http://www.biomedcentral.com/1471-213X/13/37 Copyright 2013 Pu et al.; licensee BioMed Central Ltd. Somite Dermomyotome Myotome Chicken embryo Research article 2013 ftbiomed 2013-10-27T00:20:36Z Abstract Background The myotome is the primitive skeletal muscle that forms within the embryonic metameric body wall. It can be subdivided into an epaxial and hypaxial domain. It has been shown that the formation of the epaxial myotome requires the dorsomedial lip of the dermomyotome (DML). Although the ventrolateral lip (VLL) of the dermomyotome is believed to be required for the formation of the hypaxial myotome, experimentally evidence for this statement still needs to be provided. Provision of such data would enable the resolution of a debate regarding the formation of the hypaxial dermomyotome. Two mechanisms have been proposed for this tissue. The first proposes that the intermediate dermomyotome undergoes cellular expansion thereby pushing the ventral lateral lip in a lateral direction (translocation). In contrast, the alternative view holds that the ventral lateral lip grows laterally. Results Using time lapse confocal microscopy, we observed that the GFP-labelled ventrolateral lip (VLL) of the dermomyotome grows rather than translocates in a lateral direction. The necessity of the VLL for lateral extension of the myotome was addressed by ablation studies. We found that the hypaxial myotome did not form after VLL ablation. In contrast, the removal of an intermediate portion of the dermomyotome had very little effect of the hypaxial myotome. These results demonstrate that the VLL is required for the formation of the hypaxial myotome. Conclusion Our study demonstrates that the dermomyotome ventrolateral lip is essential for the hypaxial myotome formation and supports the lip extension model. Therefore, despite being under independent signalling controls, both the dorsomedial and ventrolateral lip fulfil the same function, i.e. they extend into adjacent regions permitting the growth of the myotome. Article in Journal/Newspaper DML BioMed Central |
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Open Polar |
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BioMed Central |
op_collection_id |
ftbiomed |
language |
English |
topic |
Somite Dermomyotome Myotome Chicken embryo |
spellingShingle |
Somite Dermomyotome Myotome Chicken embryo Pu, Qin Abduelmula, Aisha Masyuk, Maryna Theiss, Carsten Schwandulla, Dieter Hans, Michael Patel, Ketan Brand-Saberi, Beate Huang, Ruijin The dermomyotome ventrolateral lip is essential for the hypaxial myotome formation |
topic_facet |
Somite Dermomyotome Myotome Chicken embryo |
description |
Abstract Background The myotome is the primitive skeletal muscle that forms within the embryonic metameric body wall. It can be subdivided into an epaxial and hypaxial domain. It has been shown that the formation of the epaxial myotome requires the dorsomedial lip of the dermomyotome (DML). Although the ventrolateral lip (VLL) of the dermomyotome is believed to be required for the formation of the hypaxial myotome, experimentally evidence for this statement still needs to be provided. Provision of such data would enable the resolution of a debate regarding the formation of the hypaxial dermomyotome. Two mechanisms have been proposed for this tissue. The first proposes that the intermediate dermomyotome undergoes cellular expansion thereby pushing the ventral lateral lip in a lateral direction (translocation). In contrast, the alternative view holds that the ventral lateral lip grows laterally. Results Using time lapse confocal microscopy, we observed that the GFP-labelled ventrolateral lip (VLL) of the dermomyotome grows rather than translocates in a lateral direction. The necessity of the VLL for lateral extension of the myotome was addressed by ablation studies. We found that the hypaxial myotome did not form after VLL ablation. In contrast, the removal of an intermediate portion of the dermomyotome had very little effect of the hypaxial myotome. These results demonstrate that the VLL is required for the formation of the hypaxial myotome. Conclusion Our study demonstrates that the dermomyotome ventrolateral lip is essential for the hypaxial myotome formation and supports the lip extension model. Therefore, despite being under independent signalling controls, both the dorsomedial and ventrolateral lip fulfil the same function, i.e. they extend into adjacent regions permitting the growth of the myotome. |
format |
Article in Journal/Newspaper |
author |
Pu, Qin Abduelmula, Aisha Masyuk, Maryna Theiss, Carsten Schwandulla, Dieter Hans, Michael Patel, Ketan Brand-Saberi, Beate Huang, Ruijin |
author_facet |
Pu, Qin Abduelmula, Aisha Masyuk, Maryna Theiss, Carsten Schwandulla, Dieter Hans, Michael Patel, Ketan Brand-Saberi, Beate Huang, Ruijin |
author_sort |
Pu, Qin |
title |
The dermomyotome ventrolateral lip is essential for the hypaxial myotome formation |
title_short |
The dermomyotome ventrolateral lip is essential for the hypaxial myotome formation |
title_full |
The dermomyotome ventrolateral lip is essential for the hypaxial myotome formation |
title_fullStr |
The dermomyotome ventrolateral lip is essential for the hypaxial myotome formation |
title_full_unstemmed |
The dermomyotome ventrolateral lip is essential for the hypaxial myotome formation |
title_sort |
dermomyotome ventrolateral lip is essential for the hypaxial myotome formation |
publisher |
BioMed Central Ltd. |
publishDate |
2013 |
url |
http://www.biomedcentral.com/1471-213X/13/37 |
genre |
DML |
genre_facet |
DML |
op_relation |
http://www.biomedcentral.com/1471-213X/13/37 |
op_rights |
Copyright 2013 Pu et al.; licensee BioMed Central Ltd. |
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1766397710410186752 |