First artificial hybrid of the eel species Anguilla australisand Anguilla anguilla
Abstract Background Studies on artificial hybridization of different Anguilla species were conducted recently, i.e. female A. australis with male A. dieffenbachii , and female A. japonica with male A. anguilla . The existence of these artificial hybrids was however not demonstrated by independent ge...
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ftbiomed:oai:biomedcentral.com:1471-213X-11-16 2023-05-15T13:27:56+02:00 First artificial hybrid of the eel species Anguilla australisand Anguilla anguilla Burgerhout, Erik Brittijn, Sebastiaan A Kurwie, Tagried Decker, Paul Dirks, Ron P Palstra, Arjan P Spaink, Herman P Van den Thillart, Guido EEJM 2011-03-13 http://www.biomedcentral.com/1471-213X/11/16 en eng BioMed Central Ltd. http://www.biomedcentral.com/1471-213X/11/16 Copyright 2011 Burgerhout et al; licensee BioMed Central Ltd. Research article 2011 ftbiomed 2011-03-27T03:44:38Z Abstract Background Studies on artificial hybridization of different Anguilla species were conducted recently, i.e. female A. australis with male A. dieffenbachii , and female A. japonica with male A. anguilla . The existence of these artificial hybrids was however not demonstrated by independent genetic methods. Two species - A. anguilla and A. australis - that are phylogenetically close but have different sexual maturation times (12-25 weeks and 6-8 weeks, respectively), were expected to produce favourable hybrids for reproduction studies. Results A modification of the protocol for the reproduction of Anguilla japonica was used to produce eight-day Anguilla australis larvae, with a success rate of 71.4%. Thus ten out of 14 females produced eggs that could be fertilized, and three batches resulted in mass hatching. Hybrid larvae from female A. australis x male A. Anguilla survived for up to seven days post fertilization (dpf). The early development of the hybrid showed typical characteristics of A. anguilla tail pigmentation at 50 hours post fertilization (hpf), indicating expression of genes derived from the father. Conclusions In this paper we describe the first production of hybrid larvae from male A. anguilla and female A. australis and their survival for up to 7 dpf. A species-specific nucleotide difference in the 18 S rDNA gene confirmed that genes from both A. australis and A. anguilla were present in the hybrids. The developmental stages of the hybrid eel embryos and larvae are described using high resolution images. Video footage also indicated a heart beat in 5-dpf larva. Article in Journal/Newspaper Anguilla anguilla BioMed Central |
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Abstract Background Studies on artificial hybridization of different Anguilla species were conducted recently, i.e. female A. australis with male A. dieffenbachii , and female A. japonica with male A. anguilla . The existence of these artificial hybrids was however not demonstrated by independent genetic methods. Two species - A. anguilla and A. australis - that are phylogenetically close but have different sexual maturation times (12-25 weeks and 6-8 weeks, respectively), were expected to produce favourable hybrids for reproduction studies. Results A modification of the protocol for the reproduction of Anguilla japonica was used to produce eight-day Anguilla australis larvae, with a success rate of 71.4%. Thus ten out of 14 females produced eggs that could be fertilized, and three batches resulted in mass hatching. Hybrid larvae from female A. australis x male A. Anguilla survived for up to seven days post fertilization (dpf). The early development of the hybrid showed typical characteristics of A. anguilla tail pigmentation at 50 hours post fertilization (hpf), indicating expression of genes derived from the father. Conclusions In this paper we describe the first production of hybrid larvae from male A. anguilla and female A. australis and their survival for up to 7 dpf. A species-specific nucleotide difference in the 18 S rDNA gene confirmed that genes from both A. australis and A. anguilla were present in the hybrids. The developmental stages of the hybrid eel embryos and larvae are described using high resolution images. Video footage also indicated a heart beat in 5-dpf larva. |
format |
Article in Journal/Newspaper |
author |
Burgerhout, Erik Brittijn, Sebastiaan A Kurwie, Tagried Decker, Paul Dirks, Ron P Palstra, Arjan P Spaink, Herman P Van den Thillart, Guido EEJM |
spellingShingle |
Burgerhout, Erik Brittijn, Sebastiaan A Kurwie, Tagried Decker, Paul Dirks, Ron P Palstra, Arjan P Spaink, Herman P Van den Thillart, Guido EEJM First artificial hybrid of the eel species Anguilla australisand Anguilla anguilla |
author_facet |
Burgerhout, Erik Brittijn, Sebastiaan A Kurwie, Tagried Decker, Paul Dirks, Ron P Palstra, Arjan P Spaink, Herman P Van den Thillart, Guido EEJM |
author_sort |
Burgerhout, Erik |
title |
First artificial hybrid of the eel species Anguilla australisand Anguilla anguilla |
title_short |
First artificial hybrid of the eel species Anguilla australisand Anguilla anguilla |
title_full |
First artificial hybrid of the eel species Anguilla australisand Anguilla anguilla |
title_fullStr |
First artificial hybrid of the eel species Anguilla australisand Anguilla anguilla |
title_full_unstemmed |
First artificial hybrid of the eel species Anguilla australisand Anguilla anguilla |
title_sort |
first artificial hybrid of the eel species anguilla australisand anguilla anguilla |
publisher |
BioMed Central Ltd. |
publishDate |
2011 |
url |
http://www.biomedcentral.com/1471-213X/11/16 |
genre |
Anguilla anguilla |
genre_facet |
Anguilla anguilla |
op_relation |
http://www.biomedcentral.com/1471-213X/11/16 |
op_rights |
Copyright 2011 Burgerhout et al; licensee BioMed Central Ltd. |
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