Estimation of antioxidant, antimicrobial activity and brine shrimp toxicity of plants collected from Oymyakon region of the Republic of Sakha (Yakutia), Russia
Abstract Background Several plants are reported to be produced various biological active compounds. Lichens from the extreme environments such as high altitude, high UV, drought and cold are believed to be synthesized unique types of secondary metabolites than the other one. Several human pathogenic...
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ftbiomed:oai:biomedcentral.com:0717-6287-47-10 2023-05-15T18:06:42+02:00 Estimation of antioxidant, antimicrobial activity and brine shrimp toxicity of plants collected from Oymyakon region of the Republic of Sakha (Yakutia), Russia Paudel, Babita Bhattarai, Hari Kim, Il Lee, Hyoungseok Sofronov, Roman Ivanova, Lena Poryadina, Lena Yim, Joung 2014-04-01 http://www.biolres.com/content/47/1/10 en eng BioMed Central Ltd. http://www.biolres.com/content/47/1/10 Copyright 2014 Paudel et al.; licensee BioMed Central Ltd. Antimicrobial Antioxidant Brine shrimp DPPH Lichen Moss Research article 2014 ftbiomed 2014-04-06T00:28:21Z Abstract Background Several plants are reported to be produced various biological active compounds. Lichens from the extreme environments such as high altitude, high UV, drought and cold are believed to be synthesized unique types of secondary metabolites than the other one. Several human pathogenic bacteria and fungi have been muted into drug resistant strains. Various synthetic antioxidant compounds have posed carcinogenic effects. This phenomenon needs further research for new effective drugs of natural origin. This manuscript aimed to screen new source of biological active compounds from plants of subarctic origin. Results A total of 114 plant species, including 80 species of higher plants, 19 species of lichens and 15 species of mosses, were collected from Oymyakon region of the Republic of Sakha (Yakutia), Russia (63˚20′N, 141˚42′E–63˚15′N, 142˚27′E). Antimicrobial, DPPH free radical scavenging and brine shrimp (Artemia salina) toxicity of all crude extract were evaluated. The obtained result was analyzed and compared with commercial standards. A total of 28 species of higher plants showed very strong antioxidant activity (DPPH IC50, 0.45-5.0 μg/mL), 13 species showed strong activity (DPPH IC50, 5-10 μg/mL), 22 species showed moderate antioxidant activity (DPPH IC50,10-20 μg/mL) and 17 species showed weak antioxidant activity (DPPH IC50 more than 20 μg/mL). Similarly, 3 species of lichen showed strong antioxidant activity, one species showed moderate and 15 species showed weak DPPH reducing activity. In addition, 4 species of mosses showed moderate antioxidant activity and 11 species showed weak antioxidant activity. Similarly, extracts of 51 species of higher plants showed antimicrobial (AM) activity against Staphylococcus aureus and 2 species showed AM activity against Candida albicans. Similarly, 11 species of lichen showed AM activity against S. aureus and 3 species showed AM activity against Escherichia coli. One species of moss showed AM activity against S. aureus. And finally, one species of higher plant Rheum compactum and one species of lichen Flavocetraria cucullata showed the toxicity against Brine shrimp larvae in 100 μg/mL of concentration. Conclusion The experimental results showed that subarctic plant species could be potential sources of various biologically active natural compounds. Article in Journal/Newspaper Republic of Sakha Subarctic Yakutia BioMed Central Sakha |
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language |
English |
topic |
Antimicrobial Antioxidant Brine shrimp DPPH Lichen Moss |
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Antimicrobial Antioxidant Brine shrimp DPPH Lichen Moss Paudel, Babita Bhattarai, Hari Kim, Il Lee, Hyoungseok Sofronov, Roman Ivanova, Lena Poryadina, Lena Yim, Joung Estimation of antioxidant, antimicrobial activity and brine shrimp toxicity of plants collected from Oymyakon region of the Republic of Sakha (Yakutia), Russia |
topic_facet |
Antimicrobial Antioxidant Brine shrimp DPPH Lichen Moss |
description |
Abstract Background Several plants are reported to be produced various biological active compounds. Lichens from the extreme environments such as high altitude, high UV, drought and cold are believed to be synthesized unique types of secondary metabolites than the other one. Several human pathogenic bacteria and fungi have been muted into drug resistant strains. Various synthetic antioxidant compounds have posed carcinogenic effects. This phenomenon needs further research for new effective drugs of natural origin. This manuscript aimed to screen new source of biological active compounds from plants of subarctic origin. Results A total of 114 plant species, including 80 species of higher plants, 19 species of lichens and 15 species of mosses, were collected from Oymyakon region of the Republic of Sakha (Yakutia), Russia (63˚20′N, 141˚42′E–63˚15′N, 142˚27′E). Antimicrobial, DPPH free radical scavenging and brine shrimp (Artemia salina) toxicity of all crude extract were evaluated. The obtained result was analyzed and compared with commercial standards. A total of 28 species of higher plants showed very strong antioxidant activity (DPPH IC50, 0.45-5.0 μg/mL), 13 species showed strong activity (DPPH IC50, 5-10 μg/mL), 22 species showed moderate antioxidant activity (DPPH IC50,10-20 μg/mL) and 17 species showed weak antioxidant activity (DPPH IC50 more than 20 μg/mL). Similarly, 3 species of lichen showed strong antioxidant activity, one species showed moderate and 15 species showed weak DPPH reducing activity. In addition, 4 species of mosses showed moderate antioxidant activity and 11 species showed weak antioxidant activity. Similarly, extracts of 51 species of higher plants showed antimicrobial (AM) activity against Staphylococcus aureus and 2 species showed AM activity against Candida albicans. Similarly, 11 species of lichen showed AM activity against S. aureus and 3 species showed AM activity against Escherichia coli. One species of moss showed AM activity against S. aureus. And finally, one species of higher plant Rheum compactum and one species of lichen Flavocetraria cucullata showed the toxicity against Brine shrimp larvae in 100 μg/mL of concentration. Conclusion The experimental results showed that subarctic plant species could be potential sources of various biologically active natural compounds. |
format |
Article in Journal/Newspaper |
author |
Paudel, Babita Bhattarai, Hari Kim, Il Lee, Hyoungseok Sofronov, Roman Ivanova, Lena Poryadina, Lena Yim, Joung |
author_facet |
Paudel, Babita Bhattarai, Hari Kim, Il Lee, Hyoungseok Sofronov, Roman Ivanova, Lena Poryadina, Lena Yim, Joung |
author_sort |
Paudel, Babita |
title |
Estimation of antioxidant, antimicrobial activity and brine shrimp toxicity of plants collected from Oymyakon region of the Republic of Sakha (Yakutia), Russia |
title_short |
Estimation of antioxidant, antimicrobial activity and brine shrimp toxicity of plants collected from Oymyakon region of the Republic of Sakha (Yakutia), Russia |
title_full |
Estimation of antioxidant, antimicrobial activity and brine shrimp toxicity of plants collected from Oymyakon region of the Republic of Sakha (Yakutia), Russia |
title_fullStr |
Estimation of antioxidant, antimicrobial activity and brine shrimp toxicity of plants collected from Oymyakon region of the Republic of Sakha (Yakutia), Russia |
title_full_unstemmed |
Estimation of antioxidant, antimicrobial activity and brine shrimp toxicity of plants collected from Oymyakon region of the Republic of Sakha (Yakutia), Russia |
title_sort |
estimation of antioxidant, antimicrobial activity and brine shrimp toxicity of plants collected from oymyakon region of the republic of sakha (yakutia), russia |
publisher |
BioMed Central Ltd. |
publishDate |
2014 |
url |
http://www.biolres.com/content/47/1/10 |
geographic |
Sakha |
geographic_facet |
Sakha |
genre |
Republic of Sakha Subarctic Yakutia |
genre_facet |
Republic of Sakha Subarctic Yakutia |
op_relation |
http://www.biolres.com/content/47/1/10 |
op_rights |
Copyright 2014 Paudel et al.; licensee BioMed Central Ltd. |
_version_ |
1766178372137779200 |