Characterization and expression of sweetfish ( Plecoglossus altivelis ) cathepsin D
Cathepsin D (CTSD) is a lysosomal acidic endoproteinase that plays an important role in immune response. In this study, we obtained sweetfish ( Plecoglossus altivelis ) CTSD (PaCTSD) via de-novo transcriptome sequencing of sweetfish macrophages. The full length cDNA sequence of PaCTSD was 1 955 bp e...
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Kunming Institute of Zoology, Chinese Academy of Sciences
2014
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ftbiolineint:cria:bioline:zr:zr14034 2023-05-15T15:32:28+02:00 Characterization and expression of sweetfish ( Plecoglossus altivelis ) cathepsin D JIAO, Yu LI, Chang-Hong LU, Xin-Jiang CHEN, Jiong Origin of publication: China 2014-07-10 html http://www.bioline.org.br/abstract?id=zr14034 en eng Kunming Institute of Zoology, Chinese Academy of Sciences http://www.zoores.ac.cn/; http://www.bioline.org.br/zr http://www.bioline.org.br/abstract?id=zr14034 Copyright 2014 - Zoological Research Zoological Research (ISSN: 2095-8137) Vol 35 Num 4 Cathepsin D Plecolossus altivelis Bacterial infection Prokaryotic expression; qRT-PCR AA 2014 ftbiolineint 2015-11-02T21:32:14Z Cathepsin D (CTSD) is a lysosomal acidic endoproteinase that plays an important role in immune response. In this study, we obtained sweetfish ( Plecoglossus altivelis ) CTSD (PaCTSD) via de-novo transcriptome sequencing of sweetfish macrophages. The full length cDNA sequence of PaCTSD was 1 955 bp encoding a propeptide of 397 amino acids. The deduced protein had a calculated molecular weight of 43.17×103. Multiple alignment with other known CTSD amino acid sequences revealed amino acid conservation through the teleosts. Phylogenetic tree analysis showed that PaCTSD grouped tightly with other fish CTSD, and was close to that of Atlantic salmon and rainbow trout. Subsequently, PaCTSD was prokaryotically expressed and refolded by the urea gradient method on a nickel-nitrilotriacetic acid column. Enzyme activity analysis showed that PaCTSD exhibited pH-dependent proteolytic activity. Quantitative real-time PCR showed that PaCTSD mRNA was expressed in all detected tissues in healthy sweetfish. The highest expression was observed in the spleen and white blood cells, followed by liver, head-kidney, kidney, intestine, gill, and muscle. After Listonella anguillarum infection, PaCTSD transcripts were up-regulated significantly in liver, spleen, white blood cells, and head-kidney of sweetfish. In summary, PaCTSD has proteolytic activity and is closely involved in the immune response of sweetfish. Other/Unknown Material Atlantic salmon Bioline International (Reference Center on Environmental Information, Brazil) |
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Open Polar |
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Bioline International (Reference Center on Environmental Information, Brazil) |
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English |
topic |
Cathepsin D Plecolossus altivelis Bacterial infection Prokaryotic expression; qRT-PCR |
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Cathepsin D Plecolossus altivelis Bacterial infection Prokaryotic expression; qRT-PCR JIAO, Yu LI, Chang-Hong LU, Xin-Jiang CHEN, Jiong Characterization and expression of sweetfish ( Plecoglossus altivelis ) cathepsin D |
topic_facet |
Cathepsin D Plecolossus altivelis Bacterial infection Prokaryotic expression; qRT-PCR |
description |
Cathepsin D (CTSD) is a lysosomal acidic endoproteinase that plays an important role in immune response. In this study, we obtained sweetfish ( Plecoglossus altivelis ) CTSD (PaCTSD) via de-novo transcriptome sequencing of sweetfish macrophages. The full length cDNA sequence of PaCTSD was 1 955 bp encoding a propeptide of 397 amino acids. The deduced protein had a calculated molecular weight of 43.17×103. Multiple alignment with other known CTSD amino acid sequences revealed amino acid conservation through the teleosts. Phylogenetic tree analysis showed that PaCTSD grouped tightly with other fish CTSD, and was close to that of Atlantic salmon and rainbow trout. Subsequently, PaCTSD was prokaryotically expressed and refolded by the urea gradient method on a nickel-nitrilotriacetic acid column. Enzyme activity analysis showed that PaCTSD exhibited pH-dependent proteolytic activity. Quantitative real-time PCR showed that PaCTSD mRNA was expressed in all detected tissues in healthy sweetfish. The highest expression was observed in the spleen and white blood cells, followed by liver, head-kidney, kidney, intestine, gill, and muscle. After Listonella anguillarum infection, PaCTSD transcripts were up-regulated significantly in liver, spleen, white blood cells, and head-kidney of sweetfish. In summary, PaCTSD has proteolytic activity and is closely involved in the immune response of sweetfish. |
format |
Other/Unknown Material |
author |
JIAO, Yu LI, Chang-Hong LU, Xin-Jiang CHEN, Jiong |
author_facet |
JIAO, Yu LI, Chang-Hong LU, Xin-Jiang CHEN, Jiong |
author_sort |
JIAO, Yu |
title |
Characterization and expression of sweetfish ( Plecoglossus altivelis ) cathepsin D |
title_short |
Characterization and expression of sweetfish ( Plecoglossus altivelis ) cathepsin D |
title_full |
Characterization and expression of sweetfish ( Plecoglossus altivelis ) cathepsin D |
title_fullStr |
Characterization and expression of sweetfish ( Plecoglossus altivelis ) cathepsin D |
title_full_unstemmed |
Characterization and expression of sweetfish ( Plecoglossus altivelis ) cathepsin D |
title_sort |
characterization and expression of sweetfish ( plecoglossus altivelis ) cathepsin d |
publisher |
Kunming Institute of Zoology, Chinese Academy of Sciences |
publishDate |
2014 |
url |
http://www.bioline.org.br/abstract?id=zr14034 |
op_coverage |
Origin of publication: China |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
Zoological Research (ISSN: 2095-8137) Vol 35 Num 4 |
op_relation |
http://www.zoores.ac.cn/; http://www.bioline.org.br/zr http://www.bioline.org.br/abstract?id=zr14034 |
op_rights |
Copyright 2014 - Zoological Research |
_version_ |
1766362964482326528 |