Cloning and expression analysis of Cathepsin L cDNA of Exopalaemon carinicauda
Based on the EST sequence from a hemocyte cDNA library, the cathepsin L cDNA of Exopalaemon carinicauda (EcCatL) was cloned by rapid amplification of cDNA ends (RACE). The EcCatL cDNA was 1136 bp in length, which contains an open reading frame (ORF) of 960 bp, encoding a 319 amino-acid polypeptide....
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Kunming Institute of Zoology, Chinese Academy of Sciences
2013
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ftbiolineint:cria:bioline:zr:zr13007 2023-05-15T17:54:32+02:00 Cloning and expression analysis of Cathepsin L cDNA of Exopalaemon carinicauda DUAN, Ya-Fei LIU, Ping LI, Ji-Tao LI, Jian GAO, Bao-Quan CHEN, Ping Origin of publication: China 2013-03-14 html http://www.bioline.org.br/abstract?id=zr13007 en eng Kunming Institute of Zoology, Chinese Academy of Sciences http://www.zoores.ac.cn/; http://www.bioline.org.br/zr http://www.bioline.org.br/abstract?id=zr13007 Copyright 2013 - Kunming Institute of Zoology, the Chinese Academy of Sciences Zoological Research (ISSN: 2095-8137) Vol 34 Num 1 Exopalaemon carinicauda Cathepsin L Gene cloning Gene expression AA 2013 ftbiolineint 2015-11-02T21:32:14Z Based on the EST sequence from a hemocyte cDNA library, the cathepsin L cDNA of Exopalaemon carinicauda (EcCatL) was cloned by rapid amplification of cDNA ends (RACE). The EcCatL cDNA was 1136 bp in length, which contains an open reading frame (ORF) of 960 bp, encoding a 319 amino-acid polypeptide. Homology analysis revealed that the amino acid sequence of EcCatL was highly conserved with its homologs in other crustaceans. The similarities of EcCatL with the CatL of Palaemonetes varians and Pandalus borealis were 92% and 76%, respectively. Phylogenetic analysis showed that EcCatL was in the same branch as that of Palaemonetes varians. The expression levels of EcCatL in different tissues were analyzed by quantitative real-time PCR. Expression of EcCatL was detected in all tested tissues of E. carinicauda, including hemocytes, gill, hepatopancreas, muscle, ovary, intestine, stomach and eyestalk, with the highest expression level in hepatopancreas. After challenged with Vibrio anguillarum or white spot syndrome virus, the expression of EcCatL were up-regulated in the hemocytes and hepatopancreas of E. carinicauda. Our results implied that EcCatL might play an important role in the prawn immune response. Other/Unknown Material Pandalus borealis Bioline International (Reference Center on Environmental Information, Brazil) |
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Open Polar |
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Bioline International (Reference Center on Environmental Information, Brazil) |
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ftbiolineint |
language |
English |
topic |
Exopalaemon carinicauda Cathepsin L Gene cloning Gene expression |
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Exopalaemon carinicauda Cathepsin L Gene cloning Gene expression DUAN, Ya-Fei LIU, Ping LI, Ji-Tao LI, Jian GAO, Bao-Quan CHEN, Ping Cloning and expression analysis of Cathepsin L cDNA of Exopalaemon carinicauda |
topic_facet |
Exopalaemon carinicauda Cathepsin L Gene cloning Gene expression |
description |
Based on the EST sequence from a hemocyte cDNA library, the cathepsin L cDNA of Exopalaemon carinicauda (EcCatL) was cloned by rapid amplification of cDNA ends (RACE). The EcCatL cDNA was 1136 bp in length, which contains an open reading frame (ORF) of 960 bp, encoding a 319 amino-acid polypeptide. Homology analysis revealed that the amino acid sequence of EcCatL was highly conserved with its homologs in other crustaceans. The similarities of EcCatL with the CatL of Palaemonetes varians and Pandalus borealis were 92% and 76%, respectively. Phylogenetic analysis showed that EcCatL was in the same branch as that of Palaemonetes varians. The expression levels of EcCatL in different tissues were analyzed by quantitative real-time PCR. Expression of EcCatL was detected in all tested tissues of E. carinicauda, including hemocytes, gill, hepatopancreas, muscle, ovary, intestine, stomach and eyestalk, with the highest expression level in hepatopancreas. After challenged with Vibrio anguillarum or white spot syndrome virus, the expression of EcCatL were up-regulated in the hemocytes and hepatopancreas of E. carinicauda. Our results implied that EcCatL might play an important role in the prawn immune response. |
format |
Other/Unknown Material |
author |
DUAN, Ya-Fei LIU, Ping LI, Ji-Tao LI, Jian GAO, Bao-Quan CHEN, Ping |
author_facet |
DUAN, Ya-Fei LIU, Ping LI, Ji-Tao LI, Jian GAO, Bao-Quan CHEN, Ping |
author_sort |
DUAN, Ya-Fei |
title |
Cloning and expression analysis of Cathepsin L cDNA of Exopalaemon carinicauda |
title_short |
Cloning and expression analysis of Cathepsin L cDNA of Exopalaemon carinicauda |
title_full |
Cloning and expression analysis of Cathepsin L cDNA of Exopalaemon carinicauda |
title_fullStr |
Cloning and expression analysis of Cathepsin L cDNA of Exopalaemon carinicauda |
title_full_unstemmed |
Cloning and expression analysis of Cathepsin L cDNA of Exopalaemon carinicauda |
title_sort |
cloning and expression analysis of cathepsin l cdna of exopalaemon carinicauda |
publisher |
Kunming Institute of Zoology, Chinese Academy of Sciences |
publishDate |
2013 |
url |
http://www.bioline.org.br/abstract?id=zr13007 |
op_coverage |
Origin of publication: China |
genre |
Pandalus borealis |
genre_facet |
Pandalus borealis |
op_source |
Zoological Research (ISSN: 2095-8137) Vol 34 Num 1 |
op_relation |
http://www.zoores.ac.cn/; http://www.bioline.org.br/zr http://www.bioline.org.br/abstract?id=zr13007 |
op_rights |
Copyright 2013 - Kunming Institute of Zoology, the Chinese Academy of Sciences |
_version_ |
1766162308849991680 |