Cloning and expression of Tbx3 gene in Siberian sturgeon, Acipenser baerii
Tbx3, a member of the TBX2 subfamily of T-box gene family, encodes a transcription factor with a highly conserved DNA-binding domain, which called T-domain. Tbx3 is involved in morphogenesis and organogenesis in vertebrates, such as limb development, heart remodeling, and neural placode differentiat...
Main Authors: | , , |
---|---|
Format: | Other/Unknown Material |
Language: | English |
Published: |
Kunming Institute of Zoology, Chinese Academy of Sciences
2012
|
Subjects: | |
Online Access: | http://www.bioline.org.br/abstract?id=zr12024 |
id |
ftbiolineint:cria:bioline:zr:zr12024 |
---|---|
record_format |
openpolar |
spelling |
ftbiolineint:cria:bioline:zr:zr12024 2023-05-15T13:01:38+02:00 Cloning and expression of Tbx3 gene in Siberian sturgeon, Acipenser baerii ZHANG, Hao FAN, Chun-Xin SONG, Jia-Kun Origin of publication: China 2012-11-08 html http://www.bioline.org.br/abstract?id=zr12024 en eng Kunming Institute of Zoology, Chinese Academy of Sciences http://www.zoores.ac.cn/; http://www.bioline.org.br/zr http://www.bioline.org.br/abstract?id=zr12024 Copyright 2012 - Kunming Institute of Zoology, the Chinese Academy of Sciences Zoological Research (ISSN: 2095-8137) Vol 33 Num 2 Acipenser baerii Chondrostei Tbx3 Structure Expression AA 2012 ftbiolineint 2015-11-02T21:32:14Z Tbx3, a member of the TBX2 subfamily of T-box gene family, encodes a transcription factor with a highly conserved DNA-binding domain, which called T-domain. Tbx3 is involved in morphogenesis and organogenesis in vertebrates, such as limb development, heart remodeling, and neural placode differentiation. In the present study, a full-length 2 908 bp Tbx3 cDNA from Acipenser baerii (AbTbx3) was obtained using RT-PCR and RACE technique, which includes a 2 166 bp complete open reading frame encoding a putative peptide of 721 amino acids. AbTbx3 shares 73.5% identity with its human homolog. Particularly, the DNA-binding domain of AbTbx3 shared 95.2% identity with human Tbx3. Phylogenetic analysis revealed that AbTbx3 was grouped with Tbx3s in other vertebrates, which were clustered with Tbx2s and separated from Tbx4/5s. The predicted secondary and three-dimensional structures of the T-domain of AbTbx3 were remarkably similar to human Tbx3. Through semi-quantity RT-PCR, the expression of AbTbx3 was first detected at blastula stage during Siberian sturgeon embryonic development, increased gradually, reached its peak at early tail-bud stage and then decreased slightly. In adult sturgeon, AbTbx3 was strongly expressed in eye, brain, gill, intestines, pectoral fin and pelvic fin, but not in liver, blood, heart, kidney and muscle. The whole mount in situ hybridization showed that AbTbx3 was mainly expressed in the otic vesicle, hindbrain, dorsal notochord, pineal organ and dorsal fin bud in the larvae of stage 37 and 43. Other/Unknown Material Acipenser baerii Siberian sturgeon Bioline International (Reference Center on Environmental Information, Brazil) |
institution |
Open Polar |
collection |
Bioline International (Reference Center on Environmental Information, Brazil) |
op_collection_id |
ftbiolineint |
language |
English |
topic |
Acipenser baerii Chondrostei Tbx3 Structure Expression |
spellingShingle |
Acipenser baerii Chondrostei Tbx3 Structure Expression ZHANG, Hao FAN, Chun-Xin SONG, Jia-Kun Cloning and expression of Tbx3 gene in Siberian sturgeon, Acipenser baerii |
topic_facet |
Acipenser baerii Chondrostei Tbx3 Structure Expression |
description |
Tbx3, a member of the TBX2 subfamily of T-box gene family, encodes a transcription factor with a highly conserved DNA-binding domain, which called T-domain. Tbx3 is involved in morphogenesis and organogenesis in vertebrates, such as limb development, heart remodeling, and neural placode differentiation. In the present study, a full-length 2 908 bp Tbx3 cDNA from Acipenser baerii (AbTbx3) was obtained using RT-PCR and RACE technique, which includes a 2 166 bp complete open reading frame encoding a putative peptide of 721 amino acids. AbTbx3 shares 73.5% identity with its human homolog. Particularly, the DNA-binding domain of AbTbx3 shared 95.2% identity with human Tbx3. Phylogenetic analysis revealed that AbTbx3 was grouped with Tbx3s in other vertebrates, which were clustered with Tbx2s and separated from Tbx4/5s. The predicted secondary and three-dimensional structures of the T-domain of AbTbx3 were remarkably similar to human Tbx3. Through semi-quantity RT-PCR, the expression of AbTbx3 was first detected at blastula stage during Siberian sturgeon embryonic development, increased gradually, reached its peak at early tail-bud stage and then decreased slightly. In adult sturgeon, AbTbx3 was strongly expressed in eye, brain, gill, intestines, pectoral fin and pelvic fin, but not in liver, blood, heart, kidney and muscle. The whole mount in situ hybridization showed that AbTbx3 was mainly expressed in the otic vesicle, hindbrain, dorsal notochord, pineal organ and dorsal fin bud in the larvae of stage 37 and 43. |
format |
Other/Unknown Material |
author |
ZHANG, Hao FAN, Chun-Xin SONG, Jia-Kun |
author_facet |
ZHANG, Hao FAN, Chun-Xin SONG, Jia-Kun |
author_sort |
ZHANG, Hao |
title |
Cloning and expression of Tbx3 gene in Siberian sturgeon, Acipenser baerii |
title_short |
Cloning and expression of Tbx3 gene in Siberian sturgeon, Acipenser baerii |
title_full |
Cloning and expression of Tbx3 gene in Siberian sturgeon, Acipenser baerii |
title_fullStr |
Cloning and expression of Tbx3 gene in Siberian sturgeon, Acipenser baerii |
title_full_unstemmed |
Cloning and expression of Tbx3 gene in Siberian sturgeon, Acipenser baerii |
title_sort |
cloning and expression of tbx3 gene in siberian sturgeon, acipenser baerii |
publisher |
Kunming Institute of Zoology, Chinese Academy of Sciences |
publishDate |
2012 |
url |
http://www.bioline.org.br/abstract?id=zr12024 |
op_coverage |
Origin of publication: China |
genre |
Acipenser baerii Siberian sturgeon |
genre_facet |
Acipenser baerii Siberian sturgeon |
op_source |
Zoological Research (ISSN: 2095-8137) Vol 33 Num 2 |
op_relation |
http://www.zoores.ac.cn/; http://www.bioline.org.br/zr http://www.bioline.org.br/abstract?id=zr12024 |
op_rights |
Copyright 2012 - Kunming Institute of Zoology, the Chinese Academy of Sciences |
_version_ |
1766282498308833280 |