Cloning and characterization of Caveolin-1 gene in pigeon, Columba livia domestica

• Main Authors: Ying, Zhang, Jian-Feng, Yu, Xing-Guo, Wang, Zhi-Liang, Gu
• Format: Other/Unknown Material
• Language: Chinese
• Published: Kunming Institute of Zoology, Chinese Academy of Sciences 2011
• Subjects: Columba livia domestica; Caveolin gene; Rapid amplification of cDNA ends; Tissue expressional pattern; Canis lupus
• Online Access: http://www.bioline.org.br/abstract?id=zr10065

Caveolins, a class of principal proteins forming the structure of caveolae in plasmalemma, were encoded by caveolins gene family. Caveolin-1 gene is a member of caveolins gene family. In the present study, a full-length of 2605 bp caveolin-1 cDNA sequence in Columba livia domestica, which included a 537 bp complete ORF encoding a 178 amino acids long putative peptide, were obtained by using RT-PCR and RACE technique. The Columba livia domestica caveolin-1 CDS shared 80.1% − 93.4% homology with Bos taurus, Canis lupus familiaris, Gallus gallus and Rattus norvegicus. Meanwhile, the putative amino acid sequence of Columba livia domestica caveolin-1 shared 85.4% − 97.2% homology with the above species. The semi-quantity RT-PCR revealed that Caveolin-1 expressions were detectable in all the Columba livia domestica tissues and the expressional level of caveolin-1 gene was high in adipose, medium in various muscles, low in liver. These results demonstrated that Caveolin-1 gene was potentially involved in some metabolic pathways in adipose and muscle.