Anti-peptide antibodies: A tool for detecting IL-8 in salmonids
Background: Interleukin 8 is a chemokine that is produced by several types of cells, like macrophages and has chemotactic activity in particular on neutrophils, playing a key role during the inflammatory process. It has been demonstrated at the molecular level that this molecule is present and conse...
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Universidad Católica de Valparaíso
2014
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ftbiolineint:cria:bioline:ej:ej12047 2023-05-15T18:09:55+02:00 Anti-peptide antibodies: A tool for detecting IL-8 in salmonids Santana, Paula Palacios, Claudia Narváez, Edgar Guzmán, Fanny Gallardo, José A. Mercado, Luis Origin of publication: Chile 2014-07-04 html http://www.bioline.org.br/abstract?id=ej12047 en eng Universidad Católica de Valparaíso http://www.ejbiotechnology.info; http://www.bioline.org.br/ej http://www.bioline.org.br/abstract?id=ej12047 Electronic Journal of Biotechnology Electronic Journal of Biotechnology (ISSN: 0717-3458) Vol 15 Num 5 anti-IL-8 fish antibodies fish chemokine rainbow trout synthetic peptide epitope AA 2014 ftbiolineint 2015-11-02T21:02:58Z Background: Interleukin 8 is a chemokine that is produced by several types of cells, like macrophages and has chemotactic activity in particular on neutrophils, playing a key role during the inflammatory process. It has been demonstrated at the molecular level that this molecule is present and conserved in several vertebrate groups, pointing its importance. Analysis of the amino acid sequence of IL-8, projected from cDNA of Salmo salar , presents homology with the sequences of mammals, poultry and lamprey, indicating the presence of a homologous molecule in higher fish. However, there is no information at protein level, which allows characterizing the regulatory role of this molecule during the immune response in fish. Results: In this work, we designed and synthesized an epitope peptide of 10 residues with a purity of 95% and mass of 1158.7 kDa, which showed a random coil structure. From this peptide it was able to generate a polyclonal mono-specific antibody which was capable of detecting the whole molecule of IL-8 in tissue and cellular model of salmonids. Conclusions: The resulting antibody is a versatile tool for detecting IL-8 by different immune techniques such as ELISA, dot blot, western blotting and immunocytofluorescence. Analysis of IL-8 at proteomic level is a useful method for characterizing immune properties of this molecule in fish. Other/Unknown Material Salmo salar Bioline International (Reference Center on Environmental Information, Brazil) |
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Open Polar |
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Bioline International (Reference Center on Environmental Information, Brazil) |
op_collection_id |
ftbiolineint |
language |
English |
topic |
anti-IL-8 fish antibodies fish chemokine rainbow trout synthetic peptide epitope |
spellingShingle |
anti-IL-8 fish antibodies fish chemokine rainbow trout synthetic peptide epitope Santana, Paula Palacios, Claudia Narváez, Edgar Guzmán, Fanny Gallardo, José A. Mercado, Luis Anti-peptide antibodies: A tool for detecting IL-8 in salmonids |
topic_facet |
anti-IL-8 fish antibodies fish chemokine rainbow trout synthetic peptide epitope |
description |
Background: Interleukin 8 is a chemokine that is produced by several types of cells, like macrophages and has chemotactic activity in particular on neutrophils, playing a key role during the inflammatory process. It has been demonstrated at the molecular level that this molecule is present and conserved in several vertebrate groups, pointing its importance. Analysis of the amino acid sequence of IL-8, projected from cDNA of Salmo salar , presents homology with the sequences of mammals, poultry and lamprey, indicating the presence of a homologous molecule in higher fish. However, there is no information at protein level, which allows characterizing the regulatory role of this molecule during the immune response in fish. Results: In this work, we designed and synthesized an epitope peptide of 10 residues with a purity of 95% and mass of 1158.7 kDa, which showed a random coil structure. From this peptide it was able to generate a polyclonal mono-specific antibody which was capable of detecting the whole molecule of IL-8 in tissue and cellular model of salmonids. Conclusions: The resulting antibody is a versatile tool for detecting IL-8 by different immune techniques such as ELISA, dot blot, western blotting and immunocytofluorescence. Analysis of IL-8 at proteomic level is a useful method for characterizing immune properties of this molecule in fish. |
format |
Other/Unknown Material |
author |
Santana, Paula Palacios, Claudia Narváez, Edgar Guzmán, Fanny Gallardo, José A. Mercado, Luis |
author_facet |
Santana, Paula Palacios, Claudia Narváez, Edgar Guzmán, Fanny Gallardo, José A. Mercado, Luis |
author_sort |
Santana, Paula |
title |
Anti-peptide antibodies: A tool for detecting IL-8 in salmonids |
title_short |
Anti-peptide antibodies: A tool for detecting IL-8 in salmonids |
title_full |
Anti-peptide antibodies: A tool for detecting IL-8 in salmonids |
title_fullStr |
Anti-peptide antibodies: A tool for detecting IL-8 in salmonids |
title_full_unstemmed |
Anti-peptide antibodies: A tool for detecting IL-8 in salmonids |
title_sort |
anti-peptide antibodies: a tool for detecting il-8 in salmonids |
publisher |
Universidad Católica de Valparaíso |
publishDate |
2014 |
url |
http://www.bioline.org.br/abstract?id=ej12047 |
op_coverage |
Origin of publication: Chile |
genre |
Salmo salar |
genre_facet |
Salmo salar |
op_source |
Electronic Journal of Biotechnology (ISSN: 0717-3458) Vol 15 Num 5 |
op_relation |
http://www.ejbiotechnology.info; http://www.bioline.org.br/ej http://www.bioline.org.br/abstract?id=ej12047 |
op_rights |
Electronic Journal of Biotechnology |
_version_ |
1766182614210707456 |