Production of phenolic metabolites by Deschampsia antarctica shoots using UV-B treatments during cultivation in a photobioreactor
Abstract Deschampsia antarctica (DA), the only species in the Gramineae family endemic to the Antarctic territory, is characterized by a combination of high levels of free endogenous phenylpropanoid compounds under normal in situ and in vitro growth conditions. In this article, we describe the desig...
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Universidad Católica de Valparaíso
2014
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ftbiolineint:cria:bioline:ej:ej12031 2023-05-15T14:00:20+02:00 Production of phenolic metabolites by Deschampsia antarctica shoots using UV-B treatments during cultivation in a photobioreactor Sequeida, Álvaro Tapia, Eduardo Ortega, Marcelo Zamora, Pablo Castro, Álvaro Montes, Christian Zúñiga, Gustavo E. Prieto, Humberto Origin of publication: Chile 2014-07-02 html http://www.bioline.org.br/abstract?id=ej12031 en eng Universidad Católica de Valparaíso http://www.ejbiotechnology.info; http://www.bioline.org.br/ej http://www.bioline.org.br/abstract?id=ej12031 Electronic Journal of Biotechnology Electronic Journal of Biotechnology (ISSN: 0717-3458) Vol 15 Num 4 Deschampsia antarctica phenolic compounds temporary immersion bioreactor; UV-B elicitation SC 2014 ftbiolineint 2015-11-02T21:02:58Z Abstract Deschampsia antarctica (DA), the only species in the Gramineae family endemic to the Antarctic territory, is characterized by a combination of high levels of free endogenous phenylpropanoid compounds under normal in situ and in vitro growth conditions. In this article, we describe the design and use of a specific temporary immersion photobioreactor to produce both increased DA biomass and secondary metabolite accumulation by UV-B elicitation during cultivation. Three min-long immersions in an induction medium applied every 4 hrs at 14ºC ± 1 and 20/4 hrs light/darkness photoperiod increased DA biomass production over previous in vitro reports. Biomass duplication was obtained at day 10.7 of culturing, and maximum total phenolics and antioxidant activity were observed after 14 day of culturing. The addition of UV-B radiation pulses for 0.5 hrs at 6 hrs intervals increased total phenolics and antioxidant activity more than 3- and 1.5- fold, respectively, compared to controls with no UV-B. Significant accumulation of scopoletin, chlorogenic acid, gallic acid and rutin was found in these plantlets. This is the first bioreactor designed to optimize biomass and phenylpropanoid production in DA. Other/Unknown Material Antarc* Antarctic Antarctica Bioline International (Reference Center on Environmental Information, Brazil) Antarctic The Antarctic |
institution |
Open Polar |
collection |
Bioline International (Reference Center on Environmental Information, Brazil) |
op_collection_id |
ftbiolineint |
language |
English |
topic |
Deschampsia antarctica phenolic compounds temporary immersion bioreactor; UV-B elicitation |
spellingShingle |
Deschampsia antarctica phenolic compounds temporary immersion bioreactor; UV-B elicitation Sequeida, Álvaro Tapia, Eduardo Ortega, Marcelo Zamora, Pablo Castro, Álvaro Montes, Christian Zúñiga, Gustavo E. Prieto, Humberto Production of phenolic metabolites by Deschampsia antarctica shoots using UV-B treatments during cultivation in a photobioreactor |
topic_facet |
Deschampsia antarctica phenolic compounds temporary immersion bioreactor; UV-B elicitation |
description |
Abstract Deschampsia antarctica (DA), the only species in the Gramineae family endemic to the Antarctic territory, is characterized by a combination of high levels of free endogenous phenylpropanoid compounds under normal in situ and in vitro growth conditions. In this article, we describe the design and use of a specific temporary immersion photobioreactor to produce both increased DA biomass and secondary metabolite accumulation by UV-B elicitation during cultivation. Three min-long immersions in an induction medium applied every 4 hrs at 14ºC ± 1 and 20/4 hrs light/darkness photoperiod increased DA biomass production over previous in vitro reports. Biomass duplication was obtained at day 10.7 of culturing, and maximum total phenolics and antioxidant activity were observed after 14 day of culturing. The addition of UV-B radiation pulses for 0.5 hrs at 6 hrs intervals increased total phenolics and antioxidant activity more than 3- and 1.5- fold, respectively, compared to controls with no UV-B. Significant accumulation of scopoletin, chlorogenic acid, gallic acid and rutin was found in these plantlets. This is the first bioreactor designed to optimize biomass and phenylpropanoid production in DA. |
format |
Other/Unknown Material |
author |
Sequeida, Álvaro Tapia, Eduardo Ortega, Marcelo Zamora, Pablo Castro, Álvaro Montes, Christian Zúñiga, Gustavo E. Prieto, Humberto |
author_facet |
Sequeida, Álvaro Tapia, Eduardo Ortega, Marcelo Zamora, Pablo Castro, Álvaro Montes, Christian Zúñiga, Gustavo E. Prieto, Humberto |
author_sort |
Sequeida, Álvaro |
title |
Production of phenolic metabolites by Deschampsia antarctica shoots using UV-B treatments during cultivation in a photobioreactor |
title_short |
Production of phenolic metabolites by Deschampsia antarctica shoots using UV-B treatments during cultivation in a photobioreactor |
title_full |
Production of phenolic metabolites by Deschampsia antarctica shoots using UV-B treatments during cultivation in a photobioreactor |
title_fullStr |
Production of phenolic metabolites by Deschampsia antarctica shoots using UV-B treatments during cultivation in a photobioreactor |
title_full_unstemmed |
Production of phenolic metabolites by Deschampsia antarctica shoots using UV-B treatments during cultivation in a photobioreactor |
title_sort |
production of phenolic metabolites by deschampsia antarctica shoots using uv-b treatments during cultivation in a photobioreactor |
publisher |
Universidad Católica de Valparaíso |
publishDate |
2014 |
url |
http://www.bioline.org.br/abstract?id=ej12031 |
op_coverage |
Origin of publication: Chile |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_source |
Electronic Journal of Biotechnology (ISSN: 0717-3458) Vol 15 Num 4 |
op_relation |
http://www.ejbiotechnology.info; http://www.bioline.org.br/ej http://www.bioline.org.br/abstract?id=ej12031 |
op_rights |
Electronic Journal of Biotechnology |
_version_ |
1766269382636339200 |