Microsatellites within genes and ESTs of the Pacific oyster Crassostrea gigas and their transferability in five other Crassostrea species
We developed 15 novel polymorphic microsatellites for the Pacific oyster Crassostrea gigas by screening genes and expressed sequence tags (ESTs) found in GenBank. The number of alleles per locus ranged from two to 24 with an average of 8.7, and the values of observed heterozygosity (Ho) and expected...
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Universidad Católica de Valparaíso
2009
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ftbiolineint:cria:bioline:ej:ej09033 2023-05-15T15:57:48+02:00 Microsatellites within genes and ESTs of the Pacific oyster Crassostrea gigas and their transferability in five other Crassostrea species Li, Q. Liu, S. Kong, L. Origin of publication: Chile 2009-07-30 html http://www.bioline.org.br/abstract?id=ej09033 en eng Universidad Católica de Valparaíso http://www.ejbiotechnology.info; http://www.bioline.org.br/ej http://www.bioline.org.br/abstract?id=ej09033 Copyright 2009 - Pontificia Universidad Católica de Valparaíso -- Chile Electronic Journal of Biotechnology (ISSN: 0717-3458) Vol 12 Num 3 indwelling medical devices infections surface attachment SC 2009 ftbiolineint 2015-11-02T21:01:57Z We developed 15 novel polymorphic microsatellites for the Pacific oyster Crassostrea gigas by screening genes and expressed sequence tags (ESTs) found in GenBank. The number of alleles per locus ranged from two to 24 with an average of 8.7, and the values of observed heterozygosity (Ho) and expected heterozygosity (He) ranged from 0.026 to 0.750 and from 0.120 to 0.947, respectively. No significant pairwise linkage disequilibrium was detected among loci and eight loci conformed to Hardy-Weinberg equilibrium. Transferability of the markers was examined on five other Crassostrea species and all the markers were amplified successfully in at least one species. These new microsatellites should be useful for population genetics, parentage analysis and genome mapping studies of C. gigas and closely related species. The nine markers identified from known genes are expected to be especially valuable for comparative mapping as type I markers. Other/Unknown Material Crassostrea gigas Pacific oyster Bioline International (Reference Center on Environmental Information, Brazil) Pacific |
institution |
Open Polar |
collection |
Bioline International (Reference Center on Environmental Information, Brazil) |
op_collection_id |
ftbiolineint |
language |
English |
topic |
indwelling medical devices infections surface attachment |
spellingShingle |
indwelling medical devices infections surface attachment Li, Q. Liu, S. Kong, L. Microsatellites within genes and ESTs of the Pacific oyster Crassostrea gigas and their transferability in five other Crassostrea species |
topic_facet |
indwelling medical devices infections surface attachment |
description |
We developed 15 novel polymorphic microsatellites for the Pacific oyster Crassostrea gigas by screening genes and expressed sequence tags (ESTs) found in GenBank. The number of alleles per locus ranged from two to 24 with an average of 8.7, and the values of observed heterozygosity (Ho) and expected heterozygosity (He) ranged from 0.026 to 0.750 and from 0.120 to 0.947, respectively. No significant pairwise linkage disequilibrium was detected among loci and eight loci conformed to Hardy-Weinberg equilibrium. Transferability of the markers was examined on five other Crassostrea species and all the markers were amplified successfully in at least one species. These new microsatellites should be useful for population genetics, parentage analysis and genome mapping studies of C. gigas and closely related species. The nine markers identified from known genes are expected to be especially valuable for comparative mapping as type I markers. |
format |
Other/Unknown Material |
author |
Li, Q. Liu, S. Kong, L. |
author_facet |
Li, Q. Liu, S. Kong, L. |
author_sort |
Li, Q. |
title |
Microsatellites within genes and ESTs of the Pacific oyster Crassostrea gigas and their transferability in five other Crassostrea species |
title_short |
Microsatellites within genes and ESTs of the Pacific oyster Crassostrea gigas and their transferability in five other Crassostrea species |
title_full |
Microsatellites within genes and ESTs of the Pacific oyster Crassostrea gigas and their transferability in five other Crassostrea species |
title_fullStr |
Microsatellites within genes and ESTs of the Pacific oyster Crassostrea gigas and their transferability in five other Crassostrea species |
title_full_unstemmed |
Microsatellites within genes and ESTs of the Pacific oyster Crassostrea gigas and their transferability in five other Crassostrea species |
title_sort |
microsatellites within genes and ests of the pacific oyster crassostrea gigas and their transferability in five other crassostrea species |
publisher |
Universidad Católica de Valparaíso |
publishDate |
2009 |
url |
http://www.bioline.org.br/abstract?id=ej09033 |
op_coverage |
Origin of publication: Chile |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Electronic Journal of Biotechnology (ISSN: 0717-3458) Vol 12 Num 3 |
op_relation |
http://www.ejbiotechnology.info; http://www.bioline.org.br/ej http://www.bioline.org.br/abstract?id=ej09033 |
op_rights |
Copyright 2009 - Pontificia Universidad Católica de Valparaíso -- Chile |
_version_ |
1766393499425439744 |