Aluminium Induced DNA-damage and Oxidative Stress in Cultures of the Marine Sponge Hymeniacidon perlevis
Aluminium is the most abundant element in the earth crust, and has no known biological function. However, it is an established neurotoxicant in its trivalent oxidation state, with exposure resulting in neurodegenerative diseases like Parkinson’s disease and presenile dementia. Although, the potentia...
Published in: | Journal of Marine Science |
---|---|
Main Authors: | , , |
Other Authors: | |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
BILINGUAL PUBLISHING CO
2019
|
Subjects: | |
Online Access: | https://ojs.bilpublishing.com/index.php/jms/article/view/1070 https://doi.org/10.30564/jms.v2i1.1070 |
id |
ftbilinugalpubl:oai:ojs.bbwpublisher.com:article/1070 |
---|---|
record_format |
openpolar |
spelling |
ftbilinugalpubl:oai:ojs.bbwpublisher.com:article/1070 2023-05-15T15:15:30+02:00 Aluminium Induced DNA-damage and Oxidative Stress in Cultures of the Marine Sponge Hymeniacidon perlevis Akpiri, Rachael Ununuma Konya, Rosline Sonayee Hodges, Nikolas John —R.U. Akpiri acknowledges receipt of a Commonwealth Scholarship Commission PhD studentship (NGCS-2014-430), United Kingdom Environmental Mutagenesis Society (UKEMS) small grant for early career research. 2019-12-30 application/pdf https://ojs.bilpublishing.com/index.php/jms/article/view/1070 https://doi.org/10.30564/jms.v2i1.1070 eng eng BILINGUAL PUBLISHING CO https://ojs.bilpublishing.com/index.php/jms/article/view/1070/1203 https://ojs.bilpublishing.com/index.php/jms/article/downloadSuppFile/1070/304 https://ojs.bilpublishing.com/index.php/jms/article/view/1070 doi:10.30564/jms.v2i1.1070 Copyright © 2019 Rachael Ununuma Akpiri, Nikolas John Hodges, Rosline Sonayee Konya https://creativecommons.org/licenses/by-nc/4.0 CC-BY-NC Journal of Marine Science; Vol 2, No 1 (2020) 2661-3239 Aluminium Comet assay DNA Damage ROS Sponge Hymeniacidon perlevis info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2019 ftbilinugalpubl https://doi.org/10.30564/jms.v2i1.1070 2021-07-27T17:20:24Z Aluminium is the most abundant element in the earth crust, and has no known biological function. However, it is an established neurotoxicant in its trivalent oxidation state, with exposure resulting in neurodegenerative diseases like Parkinson’s disease and presenile dementia. Although, the potential genotoxic and carcinogenic effects of aluminium are established in mammalian and other model system, there is however very limited information on aluminium genotoxicity in aquatic invertebrates. Mechanism of aluminium toxicity is also largely unclear. With a concentration range between 0.001– 0.05mg/L in near neutral pH water, and up to 0.5-1mg/L in an acidic water , aluminium poses a potential threat to the marine ecosystem, however it is poorly studied. This study, therefore presents for the first time, aluminium-induced DNA damage using the comet assay and reactive oxygen Species (ROS) formation using 2’, 7’-dichlorodihydrofluorescein diacetate (H2DCF-DA) assay as biomarkers of genotoxicity and oxidative stress in the inter-tidal marine sponge Hymeniacidon perlevis, respectively. H. perlevis is widely distributed in the British Isles, Mediterranean and the Arctic sea and has been reported as a model for environmental biomonitoring in aquatic ecosystem and as a suitable alternative to bivalves. In this study, cryopreserved single sponge cells of H. perlevis were cultured as viable aggregates and were thereafter treated with 0.1, 0.2, 0.3 and 0.4mg/L aluminium chloride (AlCl3) for 12 hours. Cell viability was determined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Our results showed that non-cytotoxic concentrations of AlCl3 caused a statistically significant concentration-dependent increase in the level of DNA-strand break and reactive oxygen species formation single sponge cells of H. perlevis. There was also a statistically significant positive linear correlation between aluminium-induced DNA strand break and ROS formation suggesting the involvement of ROS in the causative mechanism of the aluminium induced DNA-strand breaks observed. Article in Journal/Newspaper Arctic Bilingual Publishing Co. (BPC): E-Journals Arctic Journal of Marine Science 2 1 |
institution |
Open Polar |
collection |
Bilingual Publishing Co. (BPC): E-Journals |
op_collection_id |
ftbilinugalpubl |
language |
English |
topic |
Aluminium Comet assay DNA Damage ROS Sponge Hymeniacidon perlevis |
spellingShingle |
Aluminium Comet assay DNA Damage ROS Sponge Hymeniacidon perlevis Akpiri, Rachael Ununuma Konya, Rosline Sonayee Hodges, Nikolas John Aluminium Induced DNA-damage and Oxidative Stress in Cultures of the Marine Sponge Hymeniacidon perlevis |
topic_facet |
Aluminium Comet assay DNA Damage ROS Sponge Hymeniacidon perlevis |
description |
Aluminium is the most abundant element in the earth crust, and has no known biological function. However, it is an established neurotoxicant in its trivalent oxidation state, with exposure resulting in neurodegenerative diseases like Parkinson’s disease and presenile dementia. Although, the potential genotoxic and carcinogenic effects of aluminium are established in mammalian and other model system, there is however very limited information on aluminium genotoxicity in aquatic invertebrates. Mechanism of aluminium toxicity is also largely unclear. With a concentration range between 0.001– 0.05mg/L in near neutral pH water, and up to 0.5-1mg/L in an acidic water , aluminium poses a potential threat to the marine ecosystem, however it is poorly studied. This study, therefore presents for the first time, aluminium-induced DNA damage using the comet assay and reactive oxygen Species (ROS) formation using 2’, 7’-dichlorodihydrofluorescein diacetate (H2DCF-DA) assay as biomarkers of genotoxicity and oxidative stress in the inter-tidal marine sponge Hymeniacidon perlevis, respectively. H. perlevis is widely distributed in the British Isles, Mediterranean and the Arctic sea and has been reported as a model for environmental biomonitoring in aquatic ecosystem and as a suitable alternative to bivalves. In this study, cryopreserved single sponge cells of H. perlevis were cultured as viable aggregates and were thereafter treated with 0.1, 0.2, 0.3 and 0.4mg/L aluminium chloride (AlCl3) for 12 hours. Cell viability was determined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Our results showed that non-cytotoxic concentrations of AlCl3 caused a statistically significant concentration-dependent increase in the level of DNA-strand break and reactive oxygen species formation single sponge cells of H. perlevis. There was also a statistically significant positive linear correlation between aluminium-induced DNA strand break and ROS formation suggesting the involvement of ROS in the causative mechanism of the aluminium induced DNA-strand breaks observed. |
author2 |
—R.U. Akpiri acknowledges receipt of a Commonwealth Scholarship Commission PhD studentship (NGCS-2014-430), United Kingdom Environmental Mutagenesis Society (UKEMS) small grant for early career research. |
format |
Article in Journal/Newspaper |
author |
Akpiri, Rachael Ununuma Konya, Rosline Sonayee Hodges, Nikolas John |
author_facet |
Akpiri, Rachael Ununuma Konya, Rosline Sonayee Hodges, Nikolas John |
author_sort |
Akpiri, Rachael Ununuma |
title |
Aluminium Induced DNA-damage and Oxidative Stress in Cultures of the Marine Sponge Hymeniacidon perlevis |
title_short |
Aluminium Induced DNA-damage and Oxidative Stress in Cultures of the Marine Sponge Hymeniacidon perlevis |
title_full |
Aluminium Induced DNA-damage and Oxidative Stress in Cultures of the Marine Sponge Hymeniacidon perlevis |
title_fullStr |
Aluminium Induced DNA-damage and Oxidative Stress in Cultures of the Marine Sponge Hymeniacidon perlevis |
title_full_unstemmed |
Aluminium Induced DNA-damage and Oxidative Stress in Cultures of the Marine Sponge Hymeniacidon perlevis |
title_sort |
aluminium induced dna-damage and oxidative stress in cultures of the marine sponge hymeniacidon perlevis |
publisher |
BILINGUAL PUBLISHING CO |
publishDate |
2019 |
url |
https://ojs.bilpublishing.com/index.php/jms/article/view/1070 https://doi.org/10.30564/jms.v2i1.1070 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Marine Science; Vol 2, No 1 (2020) 2661-3239 |
op_relation |
https://ojs.bilpublishing.com/index.php/jms/article/view/1070/1203 https://ojs.bilpublishing.com/index.php/jms/article/downloadSuppFile/1070/304 https://ojs.bilpublishing.com/index.php/jms/article/view/1070 doi:10.30564/jms.v2i1.1070 |
op_rights |
Copyright © 2019 Rachael Ununuma Akpiri, Nikolas John Hodges, Rosline Sonayee Konya https://creativecommons.org/licenses/by-nc/4.0 |
op_rightsnorm |
CC-BY-NC |
op_doi |
https://doi.org/10.30564/jms.v2i1.1070 |
container_title |
Journal of Marine Science |
container_volume |
2 |
container_issue |
1 |
_version_ |
1766345869181845504 |