Isolation of Fungi from Lake Vostok Accretion Ice
Here we report the characterization of fungi from 10 accretion ice sections (3300-5100 y old) as well as two deep glacial ice sections that are close to the bottom of the glacier (1 000 000-2 000 000 y old) from the Vostok, Antarctica, 5G ice core. Fungi were characterized by fluorescence microscopy...
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ftbgstateuniv:oai:scholarworks.bgsu.edu:bio_sci_pub-1013 2023-11-05T03:36:54+01:00 Isolation of Fungi from Lake Vostok Accretion Ice Rogers, Scott O. D'Elia, Tom Veerapaneni, Ram Theraisnathan, Vincent 2009-01-01T08:00:00Z application/pdf https://scholarworks.bgsu.edu/bio_sci_pub/11 https://doi.org/10.3852/08-184 https://scholarworks.bgsu.edu/context/bio_sci_pub/article/1013/viewcontent/rogers_isolation.pdf unknown ScholarWorks@BGSU https://scholarworks.bgsu.edu/bio_sci_pub/11 doi:10.3852/08-184 https://scholarworks.bgsu.edu/context/bio_sci_pub/article/1013/viewcontent/rogers_isolation.pdf Biological Sciences Faculty Publications Antarctica Heterotrophs ITS Subglacial lake Biology text 2009 ftbgstateuniv https://doi.org/10.3852/08-184 2023-10-10T11:27:48Z Here we report the characterization of fungi from 10 accretion ice sections (3300-5100 y old) as well as two deep glacial ice sections that are close to the bottom of the glacier (1 000 000-2 000 000 y old) from the Vostok, Antarctica, 5G ice core. Fungi were characterized by fluorescence microscopy culturing and sequence analyses of ribosomal DNA internal transcribed spacers. A total of 270 fungal colonies were cultured from the accretion ice of subglacial Lake Vostok and an additional 14 from the glacial ice immediately above the accretion ice. Mean concentrations were 0-4.42 cells mL-1 ice meltwater of which 0-100% exhibited viability (as determined by fluorescence microscopy). Thirty-one unique fungal ribosomal DNA sequences (28 from accretion ice and three from glacial ice) were determined and compared to recent taxa. The results, plus tests for growth at low temperatures, indicated that Lake Vostok contains a mixture of heterotrophic psychrotolerant fungal species. This indicates that the lake is not sterile but contains a unique ecosystem. Text Antarc* Antarctica ice core Bowling Green State University: ScholarWorks@BGSU Mycologia 101 6 751 763 |
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Open Polar |
collection |
Bowling Green State University: ScholarWorks@BGSU |
op_collection_id |
ftbgstateuniv |
language |
unknown |
topic |
Antarctica Heterotrophs ITS Subglacial lake Biology |
spellingShingle |
Antarctica Heterotrophs ITS Subglacial lake Biology Rogers, Scott O. D'Elia, Tom Veerapaneni, Ram Theraisnathan, Vincent Isolation of Fungi from Lake Vostok Accretion Ice |
topic_facet |
Antarctica Heterotrophs ITS Subglacial lake Biology |
description |
Here we report the characterization of fungi from 10 accretion ice sections (3300-5100 y old) as well as two deep glacial ice sections that are close to the bottom of the glacier (1 000 000-2 000 000 y old) from the Vostok, Antarctica, 5G ice core. Fungi were characterized by fluorescence microscopy culturing and sequence analyses of ribosomal DNA internal transcribed spacers. A total of 270 fungal colonies were cultured from the accretion ice of subglacial Lake Vostok and an additional 14 from the glacial ice immediately above the accretion ice. Mean concentrations were 0-4.42 cells mL-1 ice meltwater of which 0-100% exhibited viability (as determined by fluorescence microscopy). Thirty-one unique fungal ribosomal DNA sequences (28 from accretion ice and three from glacial ice) were determined and compared to recent taxa. The results, plus tests for growth at low temperatures, indicated that Lake Vostok contains a mixture of heterotrophic psychrotolerant fungal species. This indicates that the lake is not sterile but contains a unique ecosystem. |
format |
Text |
author |
Rogers, Scott O. D'Elia, Tom Veerapaneni, Ram Theraisnathan, Vincent |
author_facet |
Rogers, Scott O. D'Elia, Tom Veerapaneni, Ram Theraisnathan, Vincent |
author_sort |
Rogers, Scott O. |
title |
Isolation of Fungi from Lake Vostok Accretion Ice |
title_short |
Isolation of Fungi from Lake Vostok Accretion Ice |
title_full |
Isolation of Fungi from Lake Vostok Accretion Ice |
title_fullStr |
Isolation of Fungi from Lake Vostok Accretion Ice |
title_full_unstemmed |
Isolation of Fungi from Lake Vostok Accretion Ice |
title_sort |
isolation of fungi from lake vostok accretion ice |
publisher |
ScholarWorks@BGSU |
publishDate |
2009 |
url |
https://scholarworks.bgsu.edu/bio_sci_pub/11 https://doi.org/10.3852/08-184 https://scholarworks.bgsu.edu/context/bio_sci_pub/article/1013/viewcontent/rogers_isolation.pdf |
genre |
Antarc* Antarctica ice core |
genre_facet |
Antarc* Antarctica ice core |
op_source |
Biological Sciences Faculty Publications |
op_relation |
https://scholarworks.bgsu.edu/bio_sci_pub/11 doi:10.3852/08-184 https://scholarworks.bgsu.edu/context/bio_sci_pub/article/1013/viewcontent/rogers_isolation.pdf |
op_doi |
https://doi.org/10.3852/08-184 |
container_title |
Mycologia |
container_volume |
101 |
container_issue |
6 |
container_start_page |
751 |
op_container_end_page |
763 |
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1781692184627511296 |