A theoretical approach for the electrochemical characterization of ciliary epithelium
The ciliary epithelium (CE) is the primary site of aqueous humor (AH) production, which results from the combined action of ultrafiltration and ionic secretion. Modulation of ionic secretion is a fundamental target for drug therapy in glaucoma, and therefore it is important to identify the main fact...
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ftbcam:oai:bird.bcamath.org:20.500.11824/1072 2023-06-11T04:10:55+02:00 A theoretical approach for the electrochemical characterization of ciliary epithelium Sacco, R. Guidoboni, G. Jerome, J.W. Bonifazi, G. Marazzi, N.M. Vercellin, A.C.V. Lang, M.S. Harris, A. 2020-01-23 application/pdf https://hdl.handle.net/20.500.11824/1072 https://doi.org/10.3390/life10020008 eng eng https://doi.org/10.3390/life10020008 2075-1729 http://hdl.handle.net/20.500.11824/1072 Reconocimiento-NoComercial-CompartirIgual 3.0 España http://creativecommons.org/licenses/by-nc-sa/3.0/es/ info:eu-repo/semantics/openAccess Aqueous Humor Ciliary Epithelium Ultrafiltration Ionic Secretion Ion Transporters Physiology Electrochemical Conditions Fluid-dynamical Conditions Mathematical Model Simulation info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2020 ftbcam https://doi.org/20.500.11824/107210.3390/life10020008 2023-04-25T23:49:22Z The ciliary epithelium (CE) is the primary site of aqueous humor (AH) production, which results from the combined action of ultrafiltration and ionic secretion. Modulation of ionic secretion is a fundamental target for drug therapy in glaucoma, and therefore it is important to identify the main factors contributing to it. Since several ion transporters have been hypothesized as relevant players in CE physiology, we propose a theoretical approach to complement experimental methods in characterizing their role in the electrochemical and fluid-dynamical conditions of CE. As a first step, we compare two model configurations that differ by (i) types of transporters included for ion exchange across the epithelial membrane, and by (ii) presence or absence of the intracellular production of carbonic acid mediated by the carbonic anhydrase enzyme. The proposed model configurations do not include neurohumoral mechanisms such as P2Y receptor dependent, cAMP or calcium dependent pathways, which occur in the ciliary epithelium bilayer and influence the activity of ion transporters, pumps and channels present in the cell membrane. Results suggest that one of the two configurations predicts sodium and potassium intracellular concentrations and transmembrane potential much more accurately than the other. Because of its quantitative prediction power, the proposed theoretical approach may help relate phenomena at the cellular scale, that cannot be accessed clinically, with phenomena occuring at the scale of the whole eye, for which clinical assessment is feasible. Article in Journal/Newspaper Carbonic acid BIRD - BCAM's Institutional Repository Data (Basque Center for Applied Mathematics) Life 10 2 8 |
institution |
Open Polar |
collection |
BIRD - BCAM's Institutional Repository Data (Basque Center for Applied Mathematics) |
op_collection_id |
ftbcam |
language |
English |
topic |
Aqueous Humor Ciliary Epithelium Ultrafiltration Ionic Secretion Ion Transporters Physiology Electrochemical Conditions Fluid-dynamical Conditions Mathematical Model Simulation |
spellingShingle |
Aqueous Humor Ciliary Epithelium Ultrafiltration Ionic Secretion Ion Transporters Physiology Electrochemical Conditions Fluid-dynamical Conditions Mathematical Model Simulation Sacco, R. Guidoboni, G. Jerome, J.W. Bonifazi, G. Marazzi, N.M. Vercellin, A.C.V. Lang, M.S. Harris, A. A theoretical approach for the electrochemical characterization of ciliary epithelium |
topic_facet |
Aqueous Humor Ciliary Epithelium Ultrafiltration Ionic Secretion Ion Transporters Physiology Electrochemical Conditions Fluid-dynamical Conditions Mathematical Model Simulation |
description |
The ciliary epithelium (CE) is the primary site of aqueous humor (AH) production, which results from the combined action of ultrafiltration and ionic secretion. Modulation of ionic secretion is a fundamental target for drug therapy in glaucoma, and therefore it is important to identify the main factors contributing to it. Since several ion transporters have been hypothesized as relevant players in CE physiology, we propose a theoretical approach to complement experimental methods in characterizing their role in the electrochemical and fluid-dynamical conditions of CE. As a first step, we compare two model configurations that differ by (i) types of transporters included for ion exchange across the epithelial membrane, and by (ii) presence or absence of the intracellular production of carbonic acid mediated by the carbonic anhydrase enzyme. The proposed model configurations do not include neurohumoral mechanisms such as P2Y receptor dependent, cAMP or calcium dependent pathways, which occur in the ciliary epithelium bilayer and influence the activity of ion transporters, pumps and channels present in the cell membrane. Results suggest that one of the two configurations predicts sodium and potassium intracellular concentrations and transmembrane potential much more accurately than the other. Because of its quantitative prediction power, the proposed theoretical approach may help relate phenomena at the cellular scale, that cannot be accessed clinically, with phenomena occuring at the scale of the whole eye, for which clinical assessment is feasible. |
format |
Article in Journal/Newspaper |
author |
Sacco, R. Guidoboni, G. Jerome, J.W. Bonifazi, G. Marazzi, N.M. Vercellin, A.C.V. Lang, M.S. Harris, A. |
author_facet |
Sacco, R. Guidoboni, G. Jerome, J.W. Bonifazi, G. Marazzi, N.M. Vercellin, A.C.V. Lang, M.S. Harris, A. |
author_sort |
Sacco, R. |
title |
A theoretical approach for the electrochemical characterization of ciliary epithelium |
title_short |
A theoretical approach for the electrochemical characterization of ciliary epithelium |
title_full |
A theoretical approach for the electrochemical characterization of ciliary epithelium |
title_fullStr |
A theoretical approach for the electrochemical characterization of ciliary epithelium |
title_full_unstemmed |
A theoretical approach for the electrochemical characterization of ciliary epithelium |
title_sort |
theoretical approach for the electrochemical characterization of ciliary epithelium |
publishDate |
2020 |
url |
https://hdl.handle.net/20.500.11824/1072 https://doi.org/10.3390/life10020008 |
genre |
Carbonic acid |
genre_facet |
Carbonic acid |
op_relation |
https://doi.org/10.3390/life10020008 2075-1729 http://hdl.handle.net/20.500.11824/1072 |
op_rights |
Reconocimiento-NoComercial-CompartirIgual 3.0 España http://creativecommons.org/licenses/by-nc-sa/3.0/es/ info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/20.500.11824/107210.3390/life10020008 |
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Life |
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10 |
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2 |
container_start_page |
8 |
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1768385667599958016 |