Biological responses from contaminants accumulated in seafood using an in vitro human intestinal and liver co-culture.
In vitro bioassays have been useful in predicting mechanisms of toxicity; however, conventional cell-based assays grown in monolayers are unavoidably poor models for human tissues due to the lack of complexity and physiological interplay observed in vivo. To address these limitations, the present st...
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ftbayloruniv:oai:null:2104/10765 2023-10-01T03:59:09+02:00 Biological responses from contaminants accumulated in seafood using an in vitro human intestinal and liver co-culture. Sutherland, Grace E., 1995- Lavado, Ramon. 2019-12-04T15:57:08Z application/pdf application/vnd.openxmlformats-officedocument.wordprocessingml.document https://hdl.handle.net/2104/10765 en eng https://hdl.handle.net/2104/10765 Worldwide access Co-culture. Caco-2. HepaRG. Cytotoxicity. Antioxidant enzymes. Galveston Bay. Red Drum. Black Drum. Spotted Trout. Oyster. Thesis text 2019 ftbayloruniv 2023-09-05T05:27:17Z In vitro bioassays have been useful in predicting mechanisms of toxicity; however, conventional cell-based assays grown in monolayers are unavoidably poor models for human tissues due to the lack of complexity and physiological interplay observed in vivo. To address these limitations, the present study utilizes a combination of human intestinal and hepatic cells in a co-culture model. The purposes of these projects are to evaluate the differences between mono- and co-culture systems related to cytotoxicity and enzyme activity, and apply the co-culture model in the screening of seafood samples collected from the Galveston Bay. It was observed that the co-culture model had greater antioxidant enzyme activity compared to that of the monoculture, suggesting that hepatocytes grown in co-culture may be better suited to facilitate the expression of enzymes in response to xenobiotic metabolism in intestinal cells. This emphasizes the importance of adequate model selection to facilitate assessment of risk. Thesis Red drum Baylor University: BEARdocs |
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Baylor University: BEARdocs |
op_collection_id |
ftbayloruniv |
language |
English |
topic |
Co-culture. Caco-2. HepaRG. Cytotoxicity. Antioxidant enzymes. Galveston Bay. Red Drum. Black Drum. Spotted Trout. Oyster. |
spellingShingle |
Co-culture. Caco-2. HepaRG. Cytotoxicity. Antioxidant enzymes. Galveston Bay. Red Drum. Black Drum. Spotted Trout. Oyster. Sutherland, Grace E., 1995- Biological responses from contaminants accumulated in seafood using an in vitro human intestinal and liver co-culture. |
topic_facet |
Co-culture. Caco-2. HepaRG. Cytotoxicity. Antioxidant enzymes. Galveston Bay. Red Drum. Black Drum. Spotted Trout. Oyster. |
description |
In vitro bioassays have been useful in predicting mechanisms of toxicity; however, conventional cell-based assays grown in monolayers are unavoidably poor models for human tissues due to the lack of complexity and physiological interplay observed in vivo. To address these limitations, the present study utilizes a combination of human intestinal and hepatic cells in a co-culture model. The purposes of these projects are to evaluate the differences between mono- and co-culture systems related to cytotoxicity and enzyme activity, and apply the co-culture model in the screening of seafood samples collected from the Galveston Bay. It was observed that the co-culture model had greater antioxidant enzyme activity compared to that of the monoculture, suggesting that hepatocytes grown in co-culture may be better suited to facilitate the expression of enzymes in response to xenobiotic metabolism in intestinal cells. This emphasizes the importance of adequate model selection to facilitate assessment of risk. |
author2 |
Lavado, Ramon. |
format |
Thesis |
author |
Sutherland, Grace E., 1995- |
author_facet |
Sutherland, Grace E., 1995- |
author_sort |
Sutherland, Grace E., 1995- |
title |
Biological responses from contaminants accumulated in seafood using an in vitro human intestinal and liver co-culture. |
title_short |
Biological responses from contaminants accumulated in seafood using an in vitro human intestinal and liver co-culture. |
title_full |
Biological responses from contaminants accumulated in seafood using an in vitro human intestinal and liver co-culture. |
title_fullStr |
Biological responses from contaminants accumulated in seafood using an in vitro human intestinal and liver co-culture. |
title_full_unstemmed |
Biological responses from contaminants accumulated in seafood using an in vitro human intestinal and liver co-culture. |
title_sort |
biological responses from contaminants accumulated in seafood using an in vitro human intestinal and liver co-culture. |
publishDate |
2019 |
url |
https://hdl.handle.net/2104/10765 |
genre |
Red drum |
genre_facet |
Red drum |
op_relation |
https://hdl.handle.net/2104/10765 |
op_rights |
Worldwide access |
_version_ |
1778532713695805440 |