Divergence between genes but limited allelic polymorphism in two MHC class II A genes in Leach’s storm-petrels Oceanodroma leucorhoa
The major histocompatibility complex (MHC) is critical to host-pathogen interactions. Class II MHC is a heterodimer, with α and β subunits encoded by different genes. The peptide-binding groove is formed by the first domain of both subunits (α1 and β1), but studies of class II variation or natural s...
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ftbatescollege:oai:scarab.bates.edu:faculty_publications-1363 2023-05-15T17:52:21+02:00 Divergence between genes but limited allelic polymorphism in two MHC class II A genes in Leach’s storm-petrels Oceanodroma leucorhoa Rand, Laura M. Woodward, Carla May, Rose Ackerman, Ross A. Tweedie, Bridget Zicarelli, T. Bruno Dearborn, Donald C 2019-09-01T07:00:00Z application/pdf https://scarab.bates.edu/faculty_publications/359 https://scarab.bates.edu/cgi/viewcontent.cgi?article=1363&context=faculty_publications unknown SCARAB https://scarab.bates.edu/faculty_publications/359 https://scarab.bates.edu/cgi/viewcontent.cgi?article=1363&context=faculty_publications Copyright © 2019, Springer-Verlag GmbH Germany, part of Springer Nature. This is the author's version of the work. This publication appears in Bates College's institutional repository by permission of the copyright owner for personal use, not for redistribution. All Faculty Scholarship Heterodimer MHC class II A Polymorphism Seabird Selection text 2019 ftbatescollege 2022-09-12T17:29:29Z The major histocompatibility complex (MHC) is critical to host-pathogen interactions. Class II MHC is a heterodimer, with α and β subunits encoded by different genes. The peptide-binding groove is formed by the first domain of both subunits (α1 and β1), but studies of class II variation or natural selection focus primarily on the β subunit and II B genes. We explored MHC II A in Leach’s storm-petrel, a seabird with two expressed, polymorphic II B genes. We found two II A genes, Ocle-DAA and Ocle-DBA, in contrast to the single II A gene in chicken and duck. In exon 2 which encodes the α1 domain, the storm-petrel II A genes differed strongly from each other but showed little within-gene polymorphism in 30 individuals: just one Ocle-DAA allele, and three Ocle-DBA alleles differing from each other by single non-synonymous substitutions. In a comparable sample, the two II B genes had nine markedly diverged alleles each. Differences between the α1 domains of Ocle-DAA and Ocle-DBA showed signatures of positive selection, but mainly at non-peptide-binding site (PBS) positions. In contrast, positive selection within and between the II B genes corresponded to putative PBS codons. Phylogenetic analysis of the conserved α2 domain did not reveal deep or well-supported lineages of II A genes in birds, in contrast to the pronounced differentiation of DQA, DPA, and DRA isotypes in mammals. This uncertain homology complicates efforts to compare levels of functional variation and modes of evolution of II A genes across taxa. Text Oceanodroma leucorhoa Bates College: SCARAB (Scholarly Communication and Research at Bates) |
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Open Polar |
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Bates College: SCARAB (Scholarly Communication and Research at Bates) |
op_collection_id |
ftbatescollege |
language |
unknown |
topic |
Heterodimer MHC class II A Polymorphism Seabird Selection |
spellingShingle |
Heterodimer MHC class II A Polymorphism Seabird Selection Rand, Laura M. Woodward, Carla May, Rose Ackerman, Ross A. Tweedie, Bridget Zicarelli, T. Bruno Dearborn, Donald C Divergence between genes but limited allelic polymorphism in two MHC class II A genes in Leach’s storm-petrels Oceanodroma leucorhoa |
topic_facet |
Heterodimer MHC class II A Polymorphism Seabird Selection |
description |
The major histocompatibility complex (MHC) is critical to host-pathogen interactions. Class II MHC is a heterodimer, with α and β subunits encoded by different genes. The peptide-binding groove is formed by the first domain of both subunits (α1 and β1), but studies of class II variation or natural selection focus primarily on the β subunit and II B genes. We explored MHC II A in Leach’s storm-petrel, a seabird with two expressed, polymorphic II B genes. We found two II A genes, Ocle-DAA and Ocle-DBA, in contrast to the single II A gene in chicken and duck. In exon 2 which encodes the α1 domain, the storm-petrel II A genes differed strongly from each other but showed little within-gene polymorphism in 30 individuals: just one Ocle-DAA allele, and three Ocle-DBA alleles differing from each other by single non-synonymous substitutions. In a comparable sample, the two II B genes had nine markedly diverged alleles each. Differences between the α1 domains of Ocle-DAA and Ocle-DBA showed signatures of positive selection, but mainly at non-peptide-binding site (PBS) positions. In contrast, positive selection within and between the II B genes corresponded to putative PBS codons. Phylogenetic analysis of the conserved α2 domain did not reveal deep or well-supported lineages of II A genes in birds, in contrast to the pronounced differentiation of DQA, DPA, and DRA isotypes in mammals. This uncertain homology complicates efforts to compare levels of functional variation and modes of evolution of II A genes across taxa. |
format |
Text |
author |
Rand, Laura M. Woodward, Carla May, Rose Ackerman, Ross A. Tweedie, Bridget Zicarelli, T. Bruno Dearborn, Donald C |
author_facet |
Rand, Laura M. Woodward, Carla May, Rose Ackerman, Ross A. Tweedie, Bridget Zicarelli, T. Bruno Dearborn, Donald C |
author_sort |
Rand, Laura M. |
title |
Divergence between genes but limited allelic polymorphism in two MHC class II A genes in Leach’s storm-petrels Oceanodroma leucorhoa |
title_short |
Divergence between genes but limited allelic polymorphism in two MHC class II A genes in Leach’s storm-petrels Oceanodroma leucorhoa |
title_full |
Divergence between genes but limited allelic polymorphism in two MHC class II A genes in Leach’s storm-petrels Oceanodroma leucorhoa |
title_fullStr |
Divergence between genes but limited allelic polymorphism in two MHC class II A genes in Leach’s storm-petrels Oceanodroma leucorhoa |
title_full_unstemmed |
Divergence between genes but limited allelic polymorphism in two MHC class II A genes in Leach’s storm-petrels Oceanodroma leucorhoa |
title_sort |
divergence between genes but limited allelic polymorphism in two mhc class ii a genes in leach’s storm-petrels oceanodroma leucorhoa |
publisher |
SCARAB |
publishDate |
2019 |
url |
https://scarab.bates.edu/faculty_publications/359 https://scarab.bates.edu/cgi/viewcontent.cgi?article=1363&context=faculty_publications |
genre |
Oceanodroma leucorhoa |
genre_facet |
Oceanodroma leucorhoa |
op_source |
All Faculty Scholarship |
op_relation |
https://scarab.bates.edu/faculty_publications/359 https://scarab.bates.edu/cgi/viewcontent.cgi?article=1363&context=faculty_publications |
op_rights |
Copyright © 2019, Springer-Verlag GmbH Germany, part of Springer Nature. This is the author's version of the work. This publication appears in Bates College's institutional repository by permission of the copyright owner for personal use, not for redistribution. |
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1766159742400462848 |