Antimycotic, antibiodeteriorative and antiaflatoxigenic potency of 2-hydroxy-4-methoxybenzaldehyde isolated from Decalepis hamiltonii on fungi causing biodeterioration of maize and sorghum grains

• Main Authors: Mohana, D.C., Raveesha, K.A.
• Format: Article in Journal/Newspaper
• Language: English
• Published: Indian Society of Mycology and Plant Pathology 2010
• Subjects: Microbiology & Biotechnology; DML
• Online Access: http://eprints-bangaloreuniversity.in/5161/; http://eprints-bangaloreuniversity.in/5161/1/Mohana%202010%20J%20Mycol%20Plant%20Pathol.pdf; https://www.researchgate.net/publication/282183515

We characterized the antimycotic, antibiodeteriorative and antiaflatoxigenic efficacy of the compd., 2-hydroxy-4-methoxybenzaldehyde isolated from Decalepis hamiltonii Wight & Arn, against different species of fungi that cause biodeterioration of corn (Zea mays L.) and sorghum (Sorghum bicolor L.) grains during storage. Fungal species that cause biodeterioration were isolated from corn and sorghum grains by agar plating method and std. blotter method (SBM). Poisoned food technique was adopted to assess fungitoxicity of the compd. against fungal isolates. For different fungal species, the inhibitory concn. (IC50), minimal inhibitory concn. (MIC) and minimal fungicidal concn. (MFC) of 2-hydroxy-4-methoxybenzaldehyde varied between 80 to 350μg/mL, 350 to 800μg/mL and 450 to 850μg/mL, resp. Among the 14 fungal species tested, Aspergillus niger, A. columnaris and A. tamari were completely inhibited at higher concns., while Fusarium oxysporum, F. proliferatum, Drechslera tetramera and Aspergillus ochraceus were completely inhibited at low concns. The inhibitory effect of the compd. was of broad-spectrum in activity and was concn.-dependent. Comparative evaluation of the active compd. with the synthetic fungicides, Blitox and Thiram, at recommended dosages revealed that the antimycotic activity of 2-hydroxy-4-methoxybenzaldehyde was superior than that of synthetic fungicides. In vivo evaluation of the compd., 2-hydroxy-4-methoxybenzaldehyde at 0.5 g/kg as seed treatment on corn and sorghum revealed that carbohydrates, water sol. proteins, lipids, aflatoxin B1 prodn. and dry matter losses (DML) were significantly conserved in treated compared with control untreated grains up to 120 days storage. D. hamiltonii being an edible plant can be exploited in the management of seed-borne pathogenic fungi and in the prevention of biodeterioration of grains and mycotoxin prodn. during storage in an eco-friendly way.