The Protein Synthesis Machinery operates at the same Expense in eurythermal and cold stenothermal Pectinids
Translationally active cell-free systems from gills of the Antarctic scallop Adamussium colbecki (Smith 1902) and the European scallop Aequipecten opercularis (Linnaeus 1758) were developed, characterised and optimised for an analysis of translational capacity. The aim was to determine the energetic...
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ftawi:oai:epic.awi.de:5788 2023-09-05T13:13:03+02:00 The Protein Synthesis Machinery operates at the same Expense in eurythermal and cold stenothermal Pectinids Storch, Daniela Pörtner, Hans-Otto 2003 application/pdf https://epic.awi.de/id/eprint/5788/ https://epic.awi.de/id/eprint/5788/1/Sto2003a.pdf https://hdl.handle.net/10013/epic.16350 https://hdl.handle.net/10013/epic.16350.d001 unknown https://epic.awi.de/id/eprint/5788/1/Sto2003a.pdf https://hdl.handle.net/10013/epic.16350.d001 Storch, D. orcid:0000-0003-3090-7554 and Pörtner, H. O. orcid:0000-0001-6535-6575 (2003) The Protein Synthesis Machinery operates at the same Expense in eurythermal and cold stenothermal Pectinids , Physiological and Biochemical Zoology, 76 (1), pp. 28-40 . hdl:10013/epic.16350 EPIC3Physiological and Biochemical Zoology, 76(1), pp. 28-40 Article isiRev 2003 ftawi 2023-08-22T19:45:41Z Translationally active cell-free systems from gills of the Antarctic scallop Adamussium colbecki (Smith 1902) and the European scallop Aequipecten opercularis (Linnaeus 1758) were developed, characterised and optimised for an analysis of translational capacity. The aim was to determine the energetic cost of protein synthesis in the in vitro cell free system by directly measuring the required energy equivalents in the lysates. Protein synthesis rate in assays conducted with lysates of A. colbecki (1,029±0,061 µmol Phe/min at 15 °C) were higher compared to lysates of A. opercularis (0,087±0,013 µmol Phe/min at 15 °C and 0,156±0,023 µmol Phe/min at 25 °C). This can in part be attributed to the naturally occurring higher RNA content in lysates of A. colbecki (0,883±0,037 mg RNA/ml lysate) compared to A. opercularis (0,468±0,013 mg RNA/ml lysate).There was no significant difference in the energetic costs of protein synthesis in cell free systems of gill lysates of the cold stenothermal A. colbecki with 4,3±0,7 energy equivalents/peptide bond formed and the eurythermal A. opercularis with 5,6±0,6 energy equivalents, indicating that there are no differences in the efficiency of the translation machinery. The energetic costs specified for protein synthesis correspond with the generally accepted theoretical value of 4 energy equivalents per peptide bond formed, especially in gill lysates of A. colbecki, whereas the value for gill lysates of A. opercularis was slightly higher. Article in Journal/Newspaper Antarc* Antarctic Alfred Wegener Institute for Polar- and Marine Research (AWI): ePIC (electronic Publication Information Center) Antarctic The Antarctic |
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Alfred Wegener Institute for Polar- and Marine Research (AWI): ePIC (electronic Publication Information Center) |
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ftawi |
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description |
Translationally active cell-free systems from gills of the Antarctic scallop Adamussium colbecki (Smith 1902) and the European scallop Aequipecten opercularis (Linnaeus 1758) were developed, characterised and optimised for an analysis of translational capacity. The aim was to determine the energetic cost of protein synthesis in the in vitro cell free system by directly measuring the required energy equivalents in the lysates. Protein synthesis rate in assays conducted with lysates of A. colbecki (1,029±0,061 µmol Phe/min at 15 °C) were higher compared to lysates of A. opercularis (0,087±0,013 µmol Phe/min at 15 °C and 0,156±0,023 µmol Phe/min at 25 °C). This can in part be attributed to the naturally occurring higher RNA content in lysates of A. colbecki (0,883±0,037 mg RNA/ml lysate) compared to A. opercularis (0,468±0,013 mg RNA/ml lysate).There was no significant difference in the energetic costs of protein synthesis in cell free systems of gill lysates of the cold stenothermal A. colbecki with 4,3±0,7 energy equivalents/peptide bond formed and the eurythermal A. opercularis with 5,6±0,6 energy equivalents, indicating that there are no differences in the efficiency of the translation machinery. The energetic costs specified for protein synthesis correspond with the generally accepted theoretical value of 4 energy equivalents per peptide bond formed, especially in gill lysates of A. colbecki, whereas the value for gill lysates of A. opercularis was slightly higher. |
format |
Article in Journal/Newspaper |
author |
Storch, Daniela Pörtner, Hans-Otto |
spellingShingle |
Storch, Daniela Pörtner, Hans-Otto The Protein Synthesis Machinery operates at the same Expense in eurythermal and cold stenothermal Pectinids |
author_facet |
Storch, Daniela Pörtner, Hans-Otto |
author_sort |
Storch, Daniela |
title |
The Protein Synthesis Machinery operates at the same Expense in eurythermal and cold stenothermal Pectinids |
title_short |
The Protein Synthesis Machinery operates at the same Expense in eurythermal and cold stenothermal Pectinids |
title_full |
The Protein Synthesis Machinery operates at the same Expense in eurythermal and cold stenothermal Pectinids |
title_fullStr |
The Protein Synthesis Machinery operates at the same Expense in eurythermal and cold stenothermal Pectinids |
title_full_unstemmed |
The Protein Synthesis Machinery operates at the same Expense in eurythermal and cold stenothermal Pectinids |
title_sort |
protein synthesis machinery operates at the same expense in eurythermal and cold stenothermal pectinids |
publishDate |
2003 |
url |
https://epic.awi.de/id/eprint/5788/ https://epic.awi.de/id/eprint/5788/1/Sto2003a.pdf https://hdl.handle.net/10013/epic.16350 https://hdl.handle.net/10013/epic.16350.d001 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
EPIC3Physiological and Biochemical Zoology, 76(1), pp. 28-40 |
op_relation |
https://epic.awi.de/id/eprint/5788/1/Sto2003a.pdf https://hdl.handle.net/10013/epic.16350.d001 Storch, D. orcid:0000-0003-3090-7554 and Pörtner, H. O. orcid:0000-0001-6535-6575 (2003) The Protein Synthesis Machinery operates at the same Expense in eurythermal and cold stenothermal Pectinids , Physiological and Biochemical Zoology, 76 (1), pp. 28-40 . hdl:10013/epic.16350 |
_version_ |
1776203140862836736 |