Bacterial Communities of Frozen Quaternary Sediments of Marine Origin on the Coast of Western Spitsbergen
The bacterial composition of permafrost samples taken during drilling of frozen marine sediments in the area of Barentsburg coal mine on the east coast of Grønfjord Bay of Western Spitsbergen has been studied. The study was based on the analysis of the V4 region of the 16S rRNA gene, carried out usi...
Published in: | Izvestiya, Atmospheric and Oceanic Physics |
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Izvestiya, Atmospheric and Oceanic Physics
2021
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Online Access: | https://epic.awi.de/id/eprint/57706/ https://epic.awi.de/id/eprint/57706/1/Karaevskaya%20et%20al%202021a%20english%20Bacterial_communities-Spitsbergen_2021.pdf https://hdl.handle.net/10013/epic.bf2ffff8-e6c3-4d7f-8582-f21afd8510f6 |
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ftawi:oai:epic.awi.de:57706 2023-06-11T04:10:35+02:00 Bacterial Communities of Frozen Quaternary Sediments of Marine Origin on the Coast of Western Spitsbergen Karaevskaya, ES Demidov, NE Kazantsev, VS Elizarov, IM Kaloshin, AG Petrov, AL Karlov, DS Schirrmeister, L Belov, AA Wetterich, S 2021-12-01 application/pdf https://epic.awi.de/id/eprint/57706/ https://epic.awi.de/id/eprint/57706/1/Karaevskaya%20et%20al%202021a%20english%20Bacterial_communities-Spitsbergen_2021.pdf https://hdl.handle.net/10013/epic.bf2ffff8-e6c3-4d7f-8582-f21afd8510f6 unknown Izvestiya, Atmospheric and Oceanic Physics https://epic.awi.de/id/eprint/57706/1/Karaevskaya%20et%20al%202021a%20english%20Bacterial_communities-Spitsbergen_2021.pdf Karaevskaya, E. , Demidov, N. , Kazantsev, V. , Elizarov, I. , Kaloshin, A. , Petrov, A. , Karlov, D. , Schirrmeister, L. , Belov, A. and Wetterich, S. (2021) Bacterial Communities of Frozen Quaternary Sediments of Marine Origin on the Coast of Western Spitsbergen , Izvestiya, Atmospheric and Oceanic Physics, 57 (8) (8), pp. 895-917 . doi:10.1134/s000143382108003x <https://doi.org/10.1134/s000143382108003x> , hdl:10013/epic.bf2ffff8-e6c3-4d7f-8582-f21afd8510f6 EPIC3Izvestiya, Atmospheric and Oceanic Physics, Izvestiya, Atmospheric and Oceanic Physics, 57 (8)(8), pp. 895-917, ISSN: 0001-4338 Article peerRev 2021 ftawi https://doi.org/10.1134/s000143382108003x 2023-04-23T23:18:38Z The bacterial composition of permafrost samples taken during drilling of frozen marine sediments in the area of Barentsburg coal mine on the east coast of Grønfjord Bay of Western Spitsbergen has been studied. The study was based on the analysis of the V4 region of the 16S rRNA gene, carried out using next generation sequencing, as well as using classical microbiological methods (direct luminescence microscopy and aerobic cultivation).The total cell number in permafrost samples ranges from 6.73 ± 0.73 × 106 to 3.37 ± 0.19 107 cells per g. The number of cultivable aerobic bacteria in frozen samples on 1/5 TSA and R2A media ranges from 0 to 6.20 ± 0.45 × 104 CFU/g. Isolates of aerobic bacteria were identified by 16S rRNA gene analysis as representatives of the genera Arthrobacter, Pseudarthrobacter, Psychrobacter, and Rhodoferax. The dominant phyla of the domain Bacteria were Actinobacteria, Proteobacteria, Chloroflexi, Nitrospirae and Firmicutes. As a result of phylogenetic analysis of the dominant operational taxonomic units, representatives of methane oxidizing, sulfate reducing bacteria, as well as heterotrophic bacteria involved in the transformation of organic matter were found. Article in Journal/Newspaper Barentsburg permafrost Spitsbergen Alfred Wegener Institute for Polar- and Marine Research (AWI): ePIC (electronic Publication Information Center) Barentsburg ENVELOPE(14.212,14.212,78.064,78.064) Izvestiya, Atmospheric and Oceanic Physics 57 8 895 917 |
institution |
Open Polar |
collection |
Alfred Wegener Institute for Polar- and Marine Research (AWI): ePIC (electronic Publication Information Center) |
op_collection_id |
ftawi |
language |
unknown |
description |
The bacterial composition of permafrost samples taken during drilling of frozen marine sediments in the area of Barentsburg coal mine on the east coast of Grønfjord Bay of Western Spitsbergen has been studied. The study was based on the analysis of the V4 region of the 16S rRNA gene, carried out using next generation sequencing, as well as using classical microbiological methods (direct luminescence microscopy and aerobic cultivation).The total cell number in permafrost samples ranges from 6.73 ± 0.73 × 106 to 3.37 ± 0.19 107 cells per g. The number of cultivable aerobic bacteria in frozen samples on 1/5 TSA and R2A media ranges from 0 to 6.20 ± 0.45 × 104 CFU/g. Isolates of aerobic bacteria were identified by 16S rRNA gene analysis as representatives of the genera Arthrobacter, Pseudarthrobacter, Psychrobacter, and Rhodoferax. The dominant phyla of the domain Bacteria were Actinobacteria, Proteobacteria, Chloroflexi, Nitrospirae and Firmicutes. As a result of phylogenetic analysis of the dominant operational taxonomic units, representatives of methane oxidizing, sulfate reducing bacteria, as well as heterotrophic bacteria involved in the transformation of organic matter were found. |
format |
Article in Journal/Newspaper |
author |
Karaevskaya, ES Demidov, NE Kazantsev, VS Elizarov, IM Kaloshin, AG Petrov, AL Karlov, DS Schirrmeister, L Belov, AA Wetterich, S |
spellingShingle |
Karaevskaya, ES Demidov, NE Kazantsev, VS Elizarov, IM Kaloshin, AG Petrov, AL Karlov, DS Schirrmeister, L Belov, AA Wetterich, S Bacterial Communities of Frozen Quaternary Sediments of Marine Origin on the Coast of Western Spitsbergen |
author_facet |
Karaevskaya, ES Demidov, NE Kazantsev, VS Elizarov, IM Kaloshin, AG Petrov, AL Karlov, DS Schirrmeister, L Belov, AA Wetterich, S |
author_sort |
Karaevskaya, ES |
title |
Bacterial Communities of Frozen Quaternary Sediments of Marine Origin on the Coast of Western Spitsbergen |
title_short |
Bacterial Communities of Frozen Quaternary Sediments of Marine Origin on the Coast of Western Spitsbergen |
title_full |
Bacterial Communities of Frozen Quaternary Sediments of Marine Origin on the Coast of Western Spitsbergen |
title_fullStr |
Bacterial Communities of Frozen Quaternary Sediments of Marine Origin on the Coast of Western Spitsbergen |
title_full_unstemmed |
Bacterial Communities of Frozen Quaternary Sediments of Marine Origin on the Coast of Western Spitsbergen |
title_sort |
bacterial communities of frozen quaternary sediments of marine origin on the coast of western spitsbergen |
publisher |
Izvestiya, Atmospheric and Oceanic Physics |
publishDate |
2021 |
url |
https://epic.awi.de/id/eprint/57706/ https://epic.awi.de/id/eprint/57706/1/Karaevskaya%20et%20al%202021a%20english%20Bacterial_communities-Spitsbergen_2021.pdf https://hdl.handle.net/10013/epic.bf2ffff8-e6c3-4d7f-8582-f21afd8510f6 |
long_lat |
ENVELOPE(14.212,14.212,78.064,78.064) |
geographic |
Barentsburg |
geographic_facet |
Barentsburg |
genre |
Barentsburg permafrost Spitsbergen |
genre_facet |
Barentsburg permafrost Spitsbergen |
op_source |
EPIC3Izvestiya, Atmospheric and Oceanic Physics, Izvestiya, Atmospheric and Oceanic Physics, 57 (8)(8), pp. 895-917, ISSN: 0001-4338 |
op_relation |
https://epic.awi.de/id/eprint/57706/1/Karaevskaya%20et%20al%202021a%20english%20Bacterial_communities-Spitsbergen_2021.pdf Karaevskaya, E. , Demidov, N. , Kazantsev, V. , Elizarov, I. , Kaloshin, A. , Petrov, A. , Karlov, D. , Schirrmeister, L. , Belov, A. and Wetterich, S. (2021) Bacterial Communities of Frozen Quaternary Sediments of Marine Origin on the Coast of Western Spitsbergen , Izvestiya, Atmospheric and Oceanic Physics, 57 (8) (8), pp. 895-917 . doi:10.1134/s000143382108003x <https://doi.org/10.1134/s000143382108003x> , hdl:10013/epic.bf2ffff8-e6c3-4d7f-8582-f21afd8510f6 |
op_doi |
https://doi.org/10.1134/s000143382108003x |
container_title |
Izvestiya, Atmospheric and Oceanic Physics |
container_volume |
57 |
container_issue |
8 |
container_start_page |
895 |
op_container_end_page |
917 |
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1768385064107769856 |