Bioactivity and Metabolome Mining of Deep-Sea Sediment-Derived Microorganisms Reveal New Hybrid PKS-NRPS Macrolactone from Aspergillus versicolor PS108-62
Despite low temperatures, poor nutrient levels and high pressure, microorganisms thrive in deep-sea environments of polar regions. The adaptability to such extreme environments renders deep-sea microorganisms an encouraging source of novel, bioactive secondary metabolites. In this study, we isolated...
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2023
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Online Access: | https://epic.awi.de/id/eprint/57600/ https://epic.awi.de/id/eprint/57600/1/marinedrugs-21-00095-1.pdf https://www.mdpi.com/1660-3397/21/2/95 https://hdl.handle.net/10013/epic.1bdebc04-d9ae-4640-882b-b3bf05b260cf |
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ftawi:oai:epic.awi.de:57600 2024-09-15T17:54:16+00:00 Bioactivity and Metabolome Mining of Deep-Sea Sediment-Derived Microorganisms Reveal New Hybrid PKS-NRPS Macrolactone from Aspergillus versicolor PS108-62 Magot, Florent Van Soen, Gwendoline Buedenbender, Larissa Li, Fengjie Soltwedel, Thomas Grauso, Laura Mangoni, Alfonso Blümel, Martina Tasdemir, Deniz 2023 application/pdf https://epic.awi.de/id/eprint/57600/ https://epic.awi.de/id/eprint/57600/1/marinedrugs-21-00095-1.pdf https://www.mdpi.com/1660-3397/21/2/95 https://hdl.handle.net/10013/epic.1bdebc04-d9ae-4640-882b-b3bf05b260cf unknown https://epic.awi.de/id/eprint/57600/1/marinedrugs-21-00095-1.pdf Magot, F. , Van Soen, G. , Buedenbender, L. , Li, F. , Soltwedel, T. orcid:0000-0002-8214-5937 , Grauso, L. , Mangoni, A. , Blümel, M. and Tasdemir, D. (2023) Bioactivity and Metabolome Mining of Deep-Sea Sediment-Derived Microorganisms Reveal New Hybrid PKS-NRPS Macrolactone from Aspergillus versicolor PS108-62 , Marine Drugs, 21 . doi:10.3390/md21020095 <https://doi.org/10.3390/md21020095> , hdl:10013/epic.1bdebc04-d9ae-4640-882b-b3bf05b260cf EPIC3Marine Drugs, 21, ISSN: 1660-3397 Article isiRev 2023 ftawi https://doi.org/10.3390/md21020095 2024-06-24T04:30:12Z Despite low temperatures, poor nutrient levels and high pressure, microorganisms thrive in deep-sea environments of polar regions. The adaptability to such extreme environments renders deep-sea microorganisms an encouraging source of novel, bioactive secondary metabolites. In this study, we isolated 77 microorganisms collected by a remotely operated vehicle from the seafloor in the Fram Strait, Arctic Ocean (depth of 2454 m). Thirty-two bacteria and six fungal strains that represented the phylogenetic diversity of the isolates were cultured using an One-Strain-Many-Compounds (OSMAC) approach. The crude EtOAc extracts were tested for antimicrobial and anticancer activities. While antibacterial activity against methicillin-resistant Staphylococcus aureus (MRSA) and Enterococcus faecium was common for many isolates, only two bacteria displayed anticancer activity, and two fungi inhibited the pathogenic yeast Candida albicans. Due to bioactivity against C. albicans and rich chemical diversity based on molecular network-based untargeted metabolomics, Aspergillus versicolor PS108-62 was selected for an in-depth chemical investigation. A chemical work-up of the SPE-fractions of its dichloromethane subextract led to the isolation of a new PKS-NRPS hybrid macrolactone, versicolide A (1), a new quinazoline (−)-isoversicomide A (3), as well as three known compounds, burnettramic acid A (2), cyclopenol (4) and cyclopenin (5). Their structures were elucidated by a combination of HRMS, NMR, [α]D, FT-IR spectroscopy and computational approaches. Due to the low amounts obtained, only compounds 2 and 4 could be tested for bioactivity, with 2 inhibiting the growth of C. albicans (IC50 7.2 µg/mL). These findings highlight, on the one hand, the vast potential of the genus Aspergillus to produce novel chemistry, particularly from underexplored ecological niches such as the Arctic deep sea, and on the other, the importance of untargeted metabolomics for selection of marine extracts for downstream chemical investigations. Article in Journal/Newspaper Arctic Ocean Fram Strait Alfred Wegener Institute for Polar- and Marine Research (AWI): ePIC (electronic Publication Information Center) Marine Drugs 21 2 95 |
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Open Polar |
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Alfred Wegener Institute for Polar- and Marine Research (AWI): ePIC (electronic Publication Information Center) |
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ftawi |
language |
unknown |
description |
Despite low temperatures, poor nutrient levels and high pressure, microorganisms thrive in deep-sea environments of polar regions. The adaptability to such extreme environments renders deep-sea microorganisms an encouraging source of novel, bioactive secondary metabolites. In this study, we isolated 77 microorganisms collected by a remotely operated vehicle from the seafloor in the Fram Strait, Arctic Ocean (depth of 2454 m). Thirty-two bacteria and six fungal strains that represented the phylogenetic diversity of the isolates were cultured using an One-Strain-Many-Compounds (OSMAC) approach. The crude EtOAc extracts were tested for antimicrobial and anticancer activities. While antibacterial activity against methicillin-resistant Staphylococcus aureus (MRSA) and Enterococcus faecium was common for many isolates, only two bacteria displayed anticancer activity, and two fungi inhibited the pathogenic yeast Candida albicans. Due to bioactivity against C. albicans and rich chemical diversity based on molecular network-based untargeted metabolomics, Aspergillus versicolor PS108-62 was selected for an in-depth chemical investigation. A chemical work-up of the SPE-fractions of its dichloromethane subextract led to the isolation of a new PKS-NRPS hybrid macrolactone, versicolide A (1), a new quinazoline (−)-isoversicomide A (3), as well as three known compounds, burnettramic acid A (2), cyclopenol (4) and cyclopenin (5). Their structures were elucidated by a combination of HRMS, NMR, [α]D, FT-IR spectroscopy and computational approaches. Due to the low amounts obtained, only compounds 2 and 4 could be tested for bioactivity, with 2 inhibiting the growth of C. albicans (IC50 7.2 µg/mL). These findings highlight, on the one hand, the vast potential of the genus Aspergillus to produce novel chemistry, particularly from underexplored ecological niches such as the Arctic deep sea, and on the other, the importance of untargeted metabolomics for selection of marine extracts for downstream chemical investigations. |
format |
Article in Journal/Newspaper |
author |
Magot, Florent Van Soen, Gwendoline Buedenbender, Larissa Li, Fengjie Soltwedel, Thomas Grauso, Laura Mangoni, Alfonso Blümel, Martina Tasdemir, Deniz |
spellingShingle |
Magot, Florent Van Soen, Gwendoline Buedenbender, Larissa Li, Fengjie Soltwedel, Thomas Grauso, Laura Mangoni, Alfonso Blümel, Martina Tasdemir, Deniz Bioactivity and Metabolome Mining of Deep-Sea Sediment-Derived Microorganisms Reveal New Hybrid PKS-NRPS Macrolactone from Aspergillus versicolor PS108-62 |
author_facet |
Magot, Florent Van Soen, Gwendoline Buedenbender, Larissa Li, Fengjie Soltwedel, Thomas Grauso, Laura Mangoni, Alfonso Blümel, Martina Tasdemir, Deniz |
author_sort |
Magot, Florent |
title |
Bioactivity and Metabolome Mining of Deep-Sea Sediment-Derived Microorganisms Reveal New Hybrid PKS-NRPS Macrolactone from Aspergillus versicolor PS108-62 |
title_short |
Bioactivity and Metabolome Mining of Deep-Sea Sediment-Derived Microorganisms Reveal New Hybrid PKS-NRPS Macrolactone from Aspergillus versicolor PS108-62 |
title_full |
Bioactivity and Metabolome Mining of Deep-Sea Sediment-Derived Microorganisms Reveal New Hybrid PKS-NRPS Macrolactone from Aspergillus versicolor PS108-62 |
title_fullStr |
Bioactivity and Metabolome Mining of Deep-Sea Sediment-Derived Microorganisms Reveal New Hybrid PKS-NRPS Macrolactone from Aspergillus versicolor PS108-62 |
title_full_unstemmed |
Bioactivity and Metabolome Mining of Deep-Sea Sediment-Derived Microorganisms Reveal New Hybrid PKS-NRPS Macrolactone from Aspergillus versicolor PS108-62 |
title_sort |
bioactivity and metabolome mining of deep-sea sediment-derived microorganisms reveal new hybrid pks-nrps macrolactone from aspergillus versicolor ps108-62 |
publishDate |
2023 |
url |
https://epic.awi.de/id/eprint/57600/ https://epic.awi.de/id/eprint/57600/1/marinedrugs-21-00095-1.pdf https://www.mdpi.com/1660-3397/21/2/95 https://hdl.handle.net/10013/epic.1bdebc04-d9ae-4640-882b-b3bf05b260cf |
genre |
Arctic Ocean Fram Strait |
genre_facet |
Arctic Ocean Fram Strait |
op_source |
EPIC3Marine Drugs, 21, ISSN: 1660-3397 |
op_relation |
https://epic.awi.de/id/eprint/57600/1/marinedrugs-21-00095-1.pdf Magot, F. , Van Soen, G. , Buedenbender, L. , Li, F. , Soltwedel, T. orcid:0000-0002-8214-5937 , Grauso, L. , Mangoni, A. , Blümel, M. and Tasdemir, D. (2023) Bioactivity and Metabolome Mining of Deep-Sea Sediment-Derived Microorganisms Reveal New Hybrid PKS-NRPS Macrolactone from Aspergillus versicolor PS108-62 , Marine Drugs, 21 . doi:10.3390/md21020095 <https://doi.org/10.3390/md21020095> , hdl:10013/epic.1bdebc04-d9ae-4640-882b-b3bf05b260cf |
op_doi |
https://doi.org/10.3390/md21020095 |
container_title |
Marine Drugs |
container_volume |
21 |
container_issue |
2 |
container_start_page |
95 |
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1810430522840580096 |