De novo transcriptome assemblies of red king crab (Paralithodes camtschaticus) and snow crab (Chionoecetes opilio) molting gland and eyestalk ganglia - Temperature effects on expression of molting and growth regulatory genes in adult red king crab
Red king crab (Paralithodes camtschaticus) and snow crab (Chionoecetes opilio) are deep-sea crustaceans widely distributed in the North Pacific and Northwest Atlantic Oceans. These giant predators have invaded the Barents Sea over the past decades, and climate-driven temperature changes may influenc...
Published in: | Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology |
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Elsevier BV
2022
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Online Access: | https://epic.awi.de/id/eprint/57508/ https://epic.awi.de/id/eprint/57508/1/1-s2.0-S1096495921001172-main.pdf https://hdl.handle.net/10013/epic.34445eac-36c0-4d1e-95cb-6adabc91fe47 https://hdl.handle.net/ |
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ftawi:oai:epic.awi.de:57508 2023-05-15T15:12:29+02:00 De novo transcriptome assemblies of red king crab (Paralithodes camtschaticus) and snow crab (Chionoecetes opilio) molting gland and eyestalk ganglia - Temperature effects on expression of molting and growth regulatory genes in adult red king crab Andersen, Øivind Johnsen, Hanne Wittmann, Astrid C Harms, Lars Thesslund, Tina Berg, Ragnhild Stenberg Siikavuopio, Sten Mykles, Donald L 2022-01 application/pdf https://epic.awi.de/id/eprint/57508/ https://epic.awi.de/id/eprint/57508/1/1-s2.0-S1096495921001172-main.pdf https://hdl.handle.net/10013/epic.34445eac-36c0-4d1e-95cb-6adabc91fe47 https://hdl.handle.net/ unknown Elsevier BV https://epic.awi.de/id/eprint/57508/1/1-s2.0-S1096495921001172-main.pdf https://hdl.handle.net/ Andersen, Ø. , Johnsen, H. , Wittmann, A. C. , Harms, L. orcid:0000-0001-7620-0613 , Thesslund, T. , Berg, R. S. , Siikavuopio, S. and Mykles, D. L. (2022) De novo transcriptome assemblies of red king crab (Paralithodes camtschaticus) and snow crab (Chionoecetes opilio) molting gland and eyestalk ganglia - Temperature effects on expression of molting and growth regulatory genes in adult red king crab , Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 257 , p. 110678 . doi:10.1016/j.cbpb.2021.110678 <https://doi.org/10.1016/j.cbpb.2021.110678> , hdl:10013/epic.34445eac-36c0-4d1e-95cb-6adabc91fe47 EPIC3Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, Elsevier BV, 257, pp. 110678-110678, ISSN: 1096-4959 Article NonPeerReviewed 2022 ftawi https://doi.org/10.1016/j.cbpb.2021.110678 2022-11-07T00:12:44Z Red king crab (Paralithodes camtschaticus) and snow crab (Chionoecetes opilio) are deep-sea crustaceans widely distributed in the North Pacific and Northwest Atlantic Oceans. These giant predators have invaded the Barents Sea over the past decades, and climate-driven temperature changes may influence their distribution and abundance in the sub-Arctic region. Molting and growth in crustaceans are strongly affected by temperature, but the underlying molecular mechanisms are little known, particularly in cold-water species. Here, we describe multiple regulatory factors in the two high-latitude crabs by developing de novo transcriptomes from the molting gland (Y-organ or YO) and eye stalk ganglia (ESG), in addition to the hepatopancreas and claw muscle of red king crab. The Halloween genes encoding the ecdysteroidogenic enzymes were expressed in YO, and the ESG contained multiple neuropeptides, including molt-inhibiting hormone (MIH), crustacean hyperglycemic hormone (CHH), and ion-transport peptide (ITP). Both crabs expressed a diversity of growth-related factors, such as mTOR, AKT, Rheb and AMPKα, and stress-responsive factors, including multiple heat shock proteins (HSPs). Temperature effects on the expression of key regulatory genes were quantified by qPCR in adult red king crab males kept at 4 °C or 10 °C for two weeks during intermolt. The Halloween genes tended to be upregulated in YO at high temperature, while the ecdysteroid receptor and several growth regulators showed tissue-specific responses to elevated temperature. Constitutive and heat-inducible HSPs were expressed in an inverse temperature-dependent manner, suggesting that adult red king crabs can acclimate to increased water temperatures. Article in Journal/Newspaper Arctic Barents Sea Chionoecetes opilio Northwest Atlantic Paralithodes camtschaticus Red king crab Snow crab Alfred Wegener Institute for Polar- and Marine Research (AWI): ePIC (electronic Publication Information Center) Arctic Barents Sea Pacific Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology 257 110678 |
institution |
Open Polar |
collection |
Alfred Wegener Institute for Polar- and Marine Research (AWI): ePIC (electronic Publication Information Center) |
op_collection_id |
ftawi |
language |
unknown |
description |
Red king crab (Paralithodes camtschaticus) and snow crab (Chionoecetes opilio) are deep-sea crustaceans widely distributed in the North Pacific and Northwest Atlantic Oceans. These giant predators have invaded the Barents Sea over the past decades, and climate-driven temperature changes may influence their distribution and abundance in the sub-Arctic region. Molting and growth in crustaceans are strongly affected by temperature, but the underlying molecular mechanisms are little known, particularly in cold-water species. Here, we describe multiple regulatory factors in the two high-latitude crabs by developing de novo transcriptomes from the molting gland (Y-organ or YO) and eye stalk ganglia (ESG), in addition to the hepatopancreas and claw muscle of red king crab. The Halloween genes encoding the ecdysteroidogenic enzymes were expressed in YO, and the ESG contained multiple neuropeptides, including molt-inhibiting hormone (MIH), crustacean hyperglycemic hormone (CHH), and ion-transport peptide (ITP). Both crabs expressed a diversity of growth-related factors, such as mTOR, AKT, Rheb and AMPKα, and stress-responsive factors, including multiple heat shock proteins (HSPs). Temperature effects on the expression of key regulatory genes were quantified by qPCR in adult red king crab males kept at 4 °C or 10 °C for two weeks during intermolt. The Halloween genes tended to be upregulated in YO at high temperature, while the ecdysteroid receptor and several growth regulators showed tissue-specific responses to elevated temperature. Constitutive and heat-inducible HSPs were expressed in an inverse temperature-dependent manner, suggesting that adult red king crabs can acclimate to increased water temperatures. |
format |
Article in Journal/Newspaper |
author |
Andersen, Øivind Johnsen, Hanne Wittmann, Astrid C Harms, Lars Thesslund, Tina Berg, Ragnhild Stenberg Siikavuopio, Sten Mykles, Donald L |
spellingShingle |
Andersen, Øivind Johnsen, Hanne Wittmann, Astrid C Harms, Lars Thesslund, Tina Berg, Ragnhild Stenberg Siikavuopio, Sten Mykles, Donald L De novo transcriptome assemblies of red king crab (Paralithodes camtschaticus) and snow crab (Chionoecetes opilio) molting gland and eyestalk ganglia - Temperature effects on expression of molting and growth regulatory genes in adult red king crab |
author_facet |
Andersen, Øivind Johnsen, Hanne Wittmann, Astrid C Harms, Lars Thesslund, Tina Berg, Ragnhild Stenberg Siikavuopio, Sten Mykles, Donald L |
author_sort |
Andersen, Øivind |
title |
De novo transcriptome assemblies of red king crab (Paralithodes camtschaticus) and snow crab (Chionoecetes opilio) molting gland and eyestalk ganglia - Temperature effects on expression of molting and growth regulatory genes in adult red king crab |
title_short |
De novo transcriptome assemblies of red king crab (Paralithodes camtschaticus) and snow crab (Chionoecetes opilio) molting gland and eyestalk ganglia - Temperature effects on expression of molting and growth regulatory genes in adult red king crab |
title_full |
De novo transcriptome assemblies of red king crab (Paralithodes camtschaticus) and snow crab (Chionoecetes opilio) molting gland and eyestalk ganglia - Temperature effects on expression of molting and growth regulatory genes in adult red king crab |
title_fullStr |
De novo transcriptome assemblies of red king crab (Paralithodes camtschaticus) and snow crab (Chionoecetes opilio) molting gland and eyestalk ganglia - Temperature effects on expression of molting and growth regulatory genes in adult red king crab |
title_full_unstemmed |
De novo transcriptome assemblies of red king crab (Paralithodes camtschaticus) and snow crab (Chionoecetes opilio) molting gland and eyestalk ganglia - Temperature effects on expression of molting and growth regulatory genes in adult red king crab |
title_sort |
de novo transcriptome assemblies of red king crab (paralithodes camtschaticus) and snow crab (chionoecetes opilio) molting gland and eyestalk ganglia - temperature effects on expression of molting and growth regulatory genes in adult red king crab |
publisher |
Elsevier BV |
publishDate |
2022 |
url |
https://epic.awi.de/id/eprint/57508/ https://epic.awi.de/id/eprint/57508/1/1-s2.0-S1096495921001172-main.pdf https://hdl.handle.net/10013/epic.34445eac-36c0-4d1e-95cb-6adabc91fe47 https://hdl.handle.net/ |
geographic |
Arctic Barents Sea Pacific |
geographic_facet |
Arctic Barents Sea Pacific |
genre |
Arctic Barents Sea Chionoecetes opilio Northwest Atlantic Paralithodes camtschaticus Red king crab Snow crab |
genre_facet |
Arctic Barents Sea Chionoecetes opilio Northwest Atlantic Paralithodes camtschaticus Red king crab Snow crab |
op_source |
EPIC3Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, Elsevier BV, 257, pp. 110678-110678, ISSN: 1096-4959 |
op_relation |
https://epic.awi.de/id/eprint/57508/1/1-s2.0-S1096495921001172-main.pdf https://hdl.handle.net/ Andersen, Ø. , Johnsen, H. , Wittmann, A. C. , Harms, L. orcid:0000-0001-7620-0613 , Thesslund, T. , Berg, R. S. , Siikavuopio, S. and Mykles, D. L. (2022) De novo transcriptome assemblies of red king crab (Paralithodes camtschaticus) and snow crab (Chionoecetes opilio) molting gland and eyestalk ganglia - Temperature effects on expression of molting and growth regulatory genes in adult red king crab , Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 257 , p. 110678 . doi:10.1016/j.cbpb.2021.110678 <https://doi.org/10.1016/j.cbpb.2021.110678> , hdl:10013/epic.34445eac-36c0-4d1e-95cb-6adabc91fe47 |
op_doi |
https://doi.org/10.1016/j.cbpb.2021.110678 |
container_title |
Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology |
container_volume |
257 |
container_start_page |
110678 |
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1766343150251540480 |