Transcriptome-proteome compendium of the Antarctic krill (Euphausia superba): Metabolic potential and repertoire of hydrolytic enzymes
The Antarctic krill (Euphausia superba Dana) is a keystone species in the Southern Ocean that uses an arsenal of hydrolases for biomacromolecule decomposition to effectively digest its omnivorous diet. The present study builds on a hybrid-assembled transcriptome (13,671 ORFs) combined with comprehen...
Published in: | PROTEOMICS |
---|---|
Main Authors: | , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | unknown |
Published: |
Wiley
2022
|
Subjects: | |
Online Access: | https://epic.awi.de/id/eprint/56680/ https://epic.awi.de/id/eprint/56680/1/Moelleretal2022.pdf https://hdl.handle.net/10013/epic.819be9d8-d7b9-460c-b256-ac549c422db4 |
id |
ftawi:oai:epic.awi.de:56680 |
---|---|
record_format |
openpolar |
spelling |
ftawi:oai:epic.awi.de:56680 2024-09-15T17:43:35+00:00 Transcriptome-proteome compendium of the Antarctic krill (Euphausia superba): Metabolic potential and repertoire of hydrolytic enzymes Möller, Lars Vainstein, Yeheven Wöhlbrand, Lars Dörries, Marvin Meyer, Bettina Sohn, Kai Rabus, Ralf 2022-07-02 application/pdf https://epic.awi.de/id/eprint/56680/ https://epic.awi.de/id/eprint/56680/1/Moelleretal2022.pdf https://hdl.handle.net/10013/epic.819be9d8-d7b9-460c-b256-ac549c422db4 unknown Wiley https://epic.awi.de/id/eprint/56680/1/Moelleretal2022.pdf Möller, L. , Vainstein, Y. , Wöhlbrand, L. , Dörries, M. , Meyer, B. orcid:0000-0001-6804-9896 , Sohn, K. and Rabus, R. (2022) Transcriptome-proteome compendium of the Antarctic krill (Euphausia superba): Metabolic potential and repertoire of hydrolytic enzymes , Proteomics . doi:10.1002/pmic.202100404 <https://doi.org/10.1002/pmic.202100404> , hdl:10013/epic.819be9d8-d7b9-460c-b256-ac549c422db4 EPIC3Proteomics, Wiley Article NonPeerReviewed 2022 ftawi https://doi.org/10.1002/pmic.202100404 2024-06-24T04:28:46Z The Antarctic krill (Euphausia superba Dana) is a keystone species in the Southern Ocean that uses an arsenal of hydrolases for biomacromolecule decomposition to effectively digest its omnivorous diet. The present study builds on a hybrid-assembled transcriptome (13,671 ORFs) combined with comprehensive proteome profiling. The analysis of individual krill compartments allowed detection of significantly more different proteins compared to that of the entire animal (1,464 vs. 294 proteins). The nearby krill sampling stations in the Bransfield Strait (Antarctic Peninsula) yielded rather uniform proteome datasets. Proteins related to energy production and lipid degradation were particularly abundant in the abdomen, agreeing with the high energy demand of muscle tissue. A total of 378 different biomacromolecule hydrolysing enzymes were detected, including 250 proteases, 99 CAZymes, 14 nucleases and 15 lipases. The large repertoire in proteases is in accord with the protein-rich diet affiliated with E. superba’s omnivorous lifestyle and complex biology. The richness in chitin-degrading enzymes allows not only digestion of zooplankton diet, but also the utilization of the discharged exoskeleton after moulting. Article in Journal/Newspaper Antarc* Antarctic Antarctic Krill Antarctic Peninsula Bransfield Strait Euphausia superba Southern Ocean Alfred Wegener Institute for Polar- and Marine Research (AWI): ePIC (electronic Publication Information Center) PROTEOMICS 2100404 |
institution |
Open Polar |
collection |
Alfred Wegener Institute for Polar- and Marine Research (AWI): ePIC (electronic Publication Information Center) |
op_collection_id |
ftawi |
language |
unknown |
description |
The Antarctic krill (Euphausia superba Dana) is a keystone species in the Southern Ocean that uses an arsenal of hydrolases for biomacromolecule decomposition to effectively digest its omnivorous diet. The present study builds on a hybrid-assembled transcriptome (13,671 ORFs) combined with comprehensive proteome profiling. The analysis of individual krill compartments allowed detection of significantly more different proteins compared to that of the entire animal (1,464 vs. 294 proteins). The nearby krill sampling stations in the Bransfield Strait (Antarctic Peninsula) yielded rather uniform proteome datasets. Proteins related to energy production and lipid degradation were particularly abundant in the abdomen, agreeing with the high energy demand of muscle tissue. A total of 378 different biomacromolecule hydrolysing enzymes were detected, including 250 proteases, 99 CAZymes, 14 nucleases and 15 lipases. The large repertoire in proteases is in accord with the protein-rich diet affiliated with E. superba’s omnivorous lifestyle and complex biology. The richness in chitin-degrading enzymes allows not only digestion of zooplankton diet, but also the utilization of the discharged exoskeleton after moulting. |
format |
Article in Journal/Newspaper |
author |
Möller, Lars Vainstein, Yeheven Wöhlbrand, Lars Dörries, Marvin Meyer, Bettina Sohn, Kai Rabus, Ralf |
spellingShingle |
Möller, Lars Vainstein, Yeheven Wöhlbrand, Lars Dörries, Marvin Meyer, Bettina Sohn, Kai Rabus, Ralf Transcriptome-proteome compendium of the Antarctic krill (Euphausia superba): Metabolic potential and repertoire of hydrolytic enzymes |
author_facet |
Möller, Lars Vainstein, Yeheven Wöhlbrand, Lars Dörries, Marvin Meyer, Bettina Sohn, Kai Rabus, Ralf |
author_sort |
Möller, Lars |
title |
Transcriptome-proteome compendium of the Antarctic krill (Euphausia superba): Metabolic potential and repertoire of hydrolytic enzymes |
title_short |
Transcriptome-proteome compendium of the Antarctic krill (Euphausia superba): Metabolic potential and repertoire of hydrolytic enzymes |
title_full |
Transcriptome-proteome compendium of the Antarctic krill (Euphausia superba): Metabolic potential and repertoire of hydrolytic enzymes |
title_fullStr |
Transcriptome-proteome compendium of the Antarctic krill (Euphausia superba): Metabolic potential and repertoire of hydrolytic enzymes |
title_full_unstemmed |
Transcriptome-proteome compendium of the Antarctic krill (Euphausia superba): Metabolic potential and repertoire of hydrolytic enzymes |
title_sort |
transcriptome-proteome compendium of the antarctic krill (euphausia superba): metabolic potential and repertoire of hydrolytic enzymes |
publisher |
Wiley |
publishDate |
2022 |
url |
https://epic.awi.de/id/eprint/56680/ https://epic.awi.de/id/eprint/56680/1/Moelleretal2022.pdf https://hdl.handle.net/10013/epic.819be9d8-d7b9-460c-b256-ac549c422db4 |
genre |
Antarc* Antarctic Antarctic Krill Antarctic Peninsula Bransfield Strait Euphausia superba Southern Ocean |
genre_facet |
Antarc* Antarctic Antarctic Krill Antarctic Peninsula Bransfield Strait Euphausia superba Southern Ocean |
op_source |
EPIC3Proteomics, Wiley |
op_relation |
https://epic.awi.de/id/eprint/56680/1/Moelleretal2022.pdf Möller, L. , Vainstein, Y. , Wöhlbrand, L. , Dörries, M. , Meyer, B. orcid:0000-0001-6804-9896 , Sohn, K. and Rabus, R. (2022) Transcriptome-proteome compendium of the Antarctic krill (Euphausia superba): Metabolic potential and repertoire of hydrolytic enzymes , Proteomics . doi:10.1002/pmic.202100404 <https://doi.org/10.1002/pmic.202100404> , hdl:10013/epic.819be9d8-d7b9-460c-b256-ac549c422db4 |
op_doi |
https://doi.org/10.1002/pmic.202100404 |
container_title |
PROTEOMICS |
container_start_page |
2100404 |
_version_ |
1810490614723117056 |