Sequence and structure comparison of ATP synthase F0 subunits 6 and 8 in notothenioid fish
Mitochondrial changes such as tight coupling of the mitochondria have facilitated sustained oxygen and respiratory activity in haemoglobin-less icefish of the Channichthyidae family. We aimed to characterise features in the sequence and structure of the proteins directly involved in proton transport...
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ftawi:oai:epic.awi.de:54750 2024-09-09T19:45:38+00:00 Sequence and structure comparison of ATP synthase F0 subunits 6 and 8 in notothenioid fish Ruzzenente, Benedetta Katyal, Gunjan Ebanks, Brad Lucassen, Magnus Papetti, Chiara Chakrabarti, Lisa 2021 application/pdf https://epic.awi.de/id/eprint/54750/ https://epic.awi.de/id/eprint/54750/1/Katyal_etal2021.pdf https://doi.org/10.1371/journal.pone.0245822 https://hdl.handle.net/10013/epic.bcc4483c-c9dd-4a62-b70c-15da46ba2c5f unknown https://epic.awi.de/id/eprint/54750/1/Katyal_etal2021.pdf Ruzzenente, B. , Katyal, G. , Ebanks, B. , Lucassen, M. orcid:0000-0003-4276-4781 , Papetti, C. and Chakrabarti, L. (2021) Sequence and structure comparison of ATP synthase F0 subunits 6 and 8 in notothenioid fish , PLOS ONE, 16 (10), e0245822 . doi:10.1371/journal.pone.0245822 <https://doi.org/10.1371/journal.pone.0245822> , hdl:10013/epic.bcc4483c-c9dd-4a62-b70c-15da46ba2c5f EPIC3PLOS ONE, 16(10), pp. e0245822, ISSN: 1932-6203 Article isiRev 2021 ftawi https://doi.org/10.1371/journal.pone.0245822 2024-06-24T04:27:29Z Mitochondrial changes such as tight coupling of the mitochondria have facilitated sustained oxygen and respiratory activity in haemoglobin-less icefish of the Channichthyidae family. We aimed to characterise features in the sequence and structure of the proteins directly involved in proton transport, which have potential physiological implications. ATP synthase subunit a (ATP6) and subunit 8 (ATP8) are proteins that function as part of the F0 component (proton pump) of the F0F1complex. Both proteins are encoded by the mitochondrial genome and involved in oxidative phosphorylation. To explore mitochondrial sequence variation for ATP6 and ATP8 we analysed sequences from C. gunnari and C. rastrospinosus and compared them with their closely related red-blooded species and eight other vertebrate species. Our comparison of the amino acid sequence of these proteins reveals important differences that could underlie aspects of the unique physiology of the icefish. In this study we find that changes in the sequence of subunit a of the icefish C. gunnari at position 35 where there is a hydrophobic alanine which is not seen in the other notothenioids we analysed. An amino acid change of this type is significant since it may have a structural impact. The biology of the haemoglobin-less icefish is necessarily unique and any insights about these animals will help to generate a better overall understanding of important physiological pathways. Article in Journal/Newspaper Icefish Alfred Wegener Institute for Polar- and Marine Research (AWI): ePIC (electronic Publication Information Center) PLOS ONE 16 10 e0245822 |
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Alfred Wegener Institute for Polar- and Marine Research (AWI): ePIC (electronic Publication Information Center) |
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ftawi |
language |
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description |
Mitochondrial changes such as tight coupling of the mitochondria have facilitated sustained oxygen and respiratory activity in haemoglobin-less icefish of the Channichthyidae family. We aimed to characterise features in the sequence and structure of the proteins directly involved in proton transport, which have potential physiological implications. ATP synthase subunit a (ATP6) and subunit 8 (ATP8) are proteins that function as part of the F0 component (proton pump) of the F0F1complex. Both proteins are encoded by the mitochondrial genome and involved in oxidative phosphorylation. To explore mitochondrial sequence variation for ATP6 and ATP8 we analysed sequences from C. gunnari and C. rastrospinosus and compared them with their closely related red-blooded species and eight other vertebrate species. Our comparison of the amino acid sequence of these proteins reveals important differences that could underlie aspects of the unique physiology of the icefish. In this study we find that changes in the sequence of subunit a of the icefish C. gunnari at position 35 where there is a hydrophobic alanine which is not seen in the other notothenioids we analysed. An amino acid change of this type is significant since it may have a structural impact. The biology of the haemoglobin-less icefish is necessarily unique and any insights about these animals will help to generate a better overall understanding of important physiological pathways. |
format |
Article in Journal/Newspaper |
author |
Ruzzenente, Benedetta Katyal, Gunjan Ebanks, Brad Lucassen, Magnus Papetti, Chiara Chakrabarti, Lisa |
spellingShingle |
Ruzzenente, Benedetta Katyal, Gunjan Ebanks, Brad Lucassen, Magnus Papetti, Chiara Chakrabarti, Lisa Sequence and structure comparison of ATP synthase F0 subunits 6 and 8 in notothenioid fish |
author_facet |
Ruzzenente, Benedetta Katyal, Gunjan Ebanks, Brad Lucassen, Magnus Papetti, Chiara Chakrabarti, Lisa |
author_sort |
Ruzzenente, Benedetta |
title |
Sequence and structure comparison of ATP synthase F0 subunits 6 and 8 in notothenioid fish |
title_short |
Sequence and structure comparison of ATP synthase F0 subunits 6 and 8 in notothenioid fish |
title_full |
Sequence and structure comparison of ATP synthase F0 subunits 6 and 8 in notothenioid fish |
title_fullStr |
Sequence and structure comparison of ATP synthase F0 subunits 6 and 8 in notothenioid fish |
title_full_unstemmed |
Sequence and structure comparison of ATP synthase F0 subunits 6 and 8 in notothenioid fish |
title_sort |
sequence and structure comparison of atp synthase f0 subunits 6 and 8 in notothenioid fish |
publishDate |
2021 |
url |
https://epic.awi.de/id/eprint/54750/ https://epic.awi.de/id/eprint/54750/1/Katyal_etal2021.pdf https://doi.org/10.1371/journal.pone.0245822 https://hdl.handle.net/10013/epic.bcc4483c-c9dd-4a62-b70c-15da46ba2c5f |
genre |
Icefish |
genre_facet |
Icefish |
op_source |
EPIC3PLOS ONE, 16(10), pp. e0245822, ISSN: 1932-6203 |
op_relation |
https://epic.awi.de/id/eprint/54750/1/Katyal_etal2021.pdf Ruzzenente, B. , Katyal, G. , Ebanks, B. , Lucassen, M. orcid:0000-0003-4276-4781 , Papetti, C. and Chakrabarti, L. (2021) Sequence and structure comparison of ATP synthase F0 subunits 6 and 8 in notothenioid fish , PLOS ONE, 16 (10), e0245822 . doi:10.1371/journal.pone.0245822 <https://doi.org/10.1371/journal.pone.0245822> , hdl:10013/epic.bcc4483c-c9dd-4a62-b70c-15da46ba2c5f |
op_doi |
https://doi.org/10.1371/journal.pone.0245822 |
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PLOS ONE |
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16 |
container_issue |
10 |
container_start_page |
e0245822 |
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1809915203554377728 |