Changes in the composition of marine and sea-ice diatoms derived from sedimentary ancient DNA of the eastern Fram Strait over the past 30 000 years
The Fram Strait is an area with a relatively low and irregular distribution of diatom microfossils in surface sediments, and thus microfossil records are scarce, rarely exceed the Holocene, and contain sparse information about past richness and taxonomic composition. These attributes make the Fram S...
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2020
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Online Access: | https://epic.awi.de/id/eprint/53016/ https://epic.awi.de/id/eprint/53016/1/os-16-1017-2020.pdf https://doi.org/10.5194/os-16-1017-2020 https://hdl.handle.net/10013/epic.93a1afe8-398e-40e7-a03a-6fe50747a249 |
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ftawi:oai:epic.awi.de:53016 2024-09-09T19:40:41+00:00 Changes in the composition of marine and sea-ice diatoms derived from sedimentary ancient DNA of the eastern Fram Strait over the past 30 000 years Zimmermann, Heike H. Stoof-Leichsenring, Kathleen R. Kruse, Stefan Müller, Juliane Stein, Ruediger Tiedemann, Ralf Herzschuh, Ulrike 2020-09-07 application/pdf https://epic.awi.de/id/eprint/53016/ https://epic.awi.de/id/eprint/53016/1/os-16-1017-2020.pdf https://doi.org/10.5194/os-16-1017-2020 https://hdl.handle.net/10013/epic.93a1afe8-398e-40e7-a03a-6fe50747a249 unknown https://epic.awi.de/id/eprint/53016/1/os-16-1017-2020.pdf Zimmermann, H. H. orcid:0000-0002-0225-5176 , Stoof-Leichsenring, K. R. orcid:0000-0002-6609-3217 , Kruse, S. orcid:0000-0003-1107-1958 , Müller, J. orcid:0000-0003-0724-4131 , Stein, R. orcid:0000-0002-4453-9564 , Tiedemann, R. orcid:0000-0001-7211-8049 and Herzschuh, U. orcid:0000-0003-0999-1261 (2020) Changes in the composition of marine and sea-ice diatoms derived from sedimentary ancient DNA of the eastern Fram Strait over the past 30 000 years , Ocean Science, 16 (5), pp. 1017-1032 . doi:10.5194/os-16-1017-2020 <https://doi.org/10.5194/os-16-1017-2020> , hdl:10013/epic.93a1afe8-398e-40e7-a03a-6fe50747a249 EPIC3Ocean Science, 16(5), pp. 1017-1032, ISSN: 1812-0792 Article isiRev 2020 ftawi https://doi.org/10.5194/os-16-1017-2020 2024-06-24T04:26:11Z The Fram Strait is an area with a relatively low and irregular distribution of diatom microfossils in surface sediments, and thus microfossil records are scarce, rarely exceed the Holocene, and contain sparse information about past richness and taxonomic composition. These attributes make the Fram Strait an ideal study site to test the utility of sedimentary ancient DNA (sedaDNA) metabarcoding. Amplifying a short, partial rbcL marker from samples of sediment core MSM05/5-712-2 resulted in 95.7 % of our sequences being assigned to diatoms across 18 different families, with 38.6 % of them being resolved to species and 25.8 % to genus level. Independent replicates show a high similarity of PCR products, especially in the oldest samples. Diatom sedaDNA richness is highest in the Late Weichselian and lowest in Mid- and Late Holocene samples. Taxonomic composition is dominated by cold-water and sea-ice-associated diatoms and suggests several reorganisations – after the Last Glacial Maximum, after the Younger Dryas, and after the Early and after the Mid-Holocene. Different sequences assigned to, amongst others, Chaetoceros socialis indicate the detectability of intra-specific diversity using sedaDNA. We detect no clear pattern between our diatom sedaDNA record and the previously published IP25 record of this core, although proportions of pennate diatoms increase with higher IP25 concentrations and proportions of Nitzschia cf. frigida exceeding 2 % of the assemblage point towards past sea-ice presence. Article in Journal/Newspaper Fram Strait Sea ice Alfred Wegener Institute for Polar- and Marine Research (AWI): ePIC (electronic Publication Information Center) Ocean Science 16 5 1017 1032 |
institution |
Open Polar |
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Alfred Wegener Institute for Polar- and Marine Research (AWI): ePIC (electronic Publication Information Center) |
op_collection_id |
ftawi |
language |
unknown |
description |
The Fram Strait is an area with a relatively low and irregular distribution of diatom microfossils in surface sediments, and thus microfossil records are scarce, rarely exceed the Holocene, and contain sparse information about past richness and taxonomic composition. These attributes make the Fram Strait an ideal study site to test the utility of sedimentary ancient DNA (sedaDNA) metabarcoding. Amplifying a short, partial rbcL marker from samples of sediment core MSM05/5-712-2 resulted in 95.7 % of our sequences being assigned to diatoms across 18 different families, with 38.6 % of them being resolved to species and 25.8 % to genus level. Independent replicates show a high similarity of PCR products, especially in the oldest samples. Diatom sedaDNA richness is highest in the Late Weichselian and lowest in Mid- and Late Holocene samples. Taxonomic composition is dominated by cold-water and sea-ice-associated diatoms and suggests several reorganisations – after the Last Glacial Maximum, after the Younger Dryas, and after the Early and after the Mid-Holocene. Different sequences assigned to, amongst others, Chaetoceros socialis indicate the detectability of intra-specific diversity using sedaDNA. We detect no clear pattern between our diatom sedaDNA record and the previously published IP25 record of this core, although proportions of pennate diatoms increase with higher IP25 concentrations and proportions of Nitzschia cf. frigida exceeding 2 % of the assemblage point towards past sea-ice presence. |
format |
Article in Journal/Newspaper |
author |
Zimmermann, Heike H. Stoof-Leichsenring, Kathleen R. Kruse, Stefan Müller, Juliane Stein, Ruediger Tiedemann, Ralf Herzschuh, Ulrike |
spellingShingle |
Zimmermann, Heike H. Stoof-Leichsenring, Kathleen R. Kruse, Stefan Müller, Juliane Stein, Ruediger Tiedemann, Ralf Herzschuh, Ulrike Changes in the composition of marine and sea-ice diatoms derived from sedimentary ancient DNA of the eastern Fram Strait over the past 30 000 years |
author_facet |
Zimmermann, Heike H. Stoof-Leichsenring, Kathleen R. Kruse, Stefan Müller, Juliane Stein, Ruediger Tiedemann, Ralf Herzschuh, Ulrike |
author_sort |
Zimmermann, Heike H. |
title |
Changes in the composition of marine and sea-ice diatoms derived from sedimentary ancient DNA of the eastern Fram Strait over the past 30 000 years |
title_short |
Changes in the composition of marine and sea-ice diatoms derived from sedimentary ancient DNA of the eastern Fram Strait over the past 30 000 years |
title_full |
Changes in the composition of marine and sea-ice diatoms derived from sedimentary ancient DNA of the eastern Fram Strait over the past 30 000 years |
title_fullStr |
Changes in the composition of marine and sea-ice diatoms derived from sedimentary ancient DNA of the eastern Fram Strait over the past 30 000 years |
title_full_unstemmed |
Changes in the composition of marine and sea-ice diatoms derived from sedimentary ancient DNA of the eastern Fram Strait over the past 30 000 years |
title_sort |
changes in the composition of marine and sea-ice diatoms derived from sedimentary ancient dna of the eastern fram strait over the past 30 000 years |
publishDate |
2020 |
url |
https://epic.awi.de/id/eprint/53016/ https://epic.awi.de/id/eprint/53016/1/os-16-1017-2020.pdf https://doi.org/10.5194/os-16-1017-2020 https://hdl.handle.net/10013/epic.93a1afe8-398e-40e7-a03a-6fe50747a249 |
genre |
Fram Strait Sea ice |
genre_facet |
Fram Strait Sea ice |
op_source |
EPIC3Ocean Science, 16(5), pp. 1017-1032, ISSN: 1812-0792 |
op_relation |
https://epic.awi.de/id/eprint/53016/1/os-16-1017-2020.pdf Zimmermann, H. H. orcid:0000-0002-0225-5176 , Stoof-Leichsenring, K. R. orcid:0000-0002-6609-3217 , Kruse, S. orcid:0000-0003-1107-1958 , Müller, J. orcid:0000-0003-0724-4131 , Stein, R. orcid:0000-0002-4453-9564 , Tiedemann, R. orcid:0000-0001-7211-8049 and Herzschuh, U. orcid:0000-0003-0999-1261 (2020) Changes in the composition of marine and sea-ice diatoms derived from sedimentary ancient DNA of the eastern Fram Strait over the past 30 000 years , Ocean Science, 16 (5), pp. 1017-1032 . doi:10.5194/os-16-1017-2020 <https://doi.org/10.5194/os-16-1017-2020> , hdl:10013/epic.93a1afe8-398e-40e7-a03a-6fe50747a249 |
op_doi |
https://doi.org/10.5194/os-16-1017-2020 |
container_title |
Ocean Science |
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16 |
container_issue |
5 |
container_start_page |
1017 |
op_container_end_page |
1032 |
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1809910085639471104 |