Is geographical variation driving the transcriptomic responses to multiple stressors in the kelp Saccharina latissima
Background: Kelps (Laminariales, Phaeophyceae) are brown macroalgae of utmost ecological, and increasingly economic, importance on temperate to polar rocky shores. Omics approaches in brown algae are still scarce and knowledge of their acclimation mechanisms to the changing conditions experienced in...
Published in: | BMC Plant Biology |
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BIOMED CENTRAL LTD
2019
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Online Access: | https://epic.awi.de/id/eprint/50796/ https://epic.awi.de/id/eprint/50796/1/Monteiro_etal_2019_BMCPlantBiology.pdf https://hdl.handle.net/10013/epic.1d7c3264-439c-44dc-8237-540bbc7e518f https://hdl.handle.net/ |
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ftawi:oai:epic.awi.de:50796 2023-05-15T15:17:38+02:00 Is geographical variation driving the transcriptomic responses to multiple stressors in the kelp Saccharina latissima Monteiro, Catia Li, Huiru Bischof, Kai Bartsch, Inka Valentin, Klaus-Ulrich Corre, Erwan Collén, Jonas Harms, Lars Glöckner, Gernot Heinrich, Sandra 2019 application/pdf https://epic.awi.de/id/eprint/50796/ https://epic.awi.de/id/eprint/50796/1/Monteiro_etal_2019_BMCPlantBiology.pdf https://hdl.handle.net/10013/epic.1d7c3264-439c-44dc-8237-540bbc7e518f https://hdl.handle.net/ unknown BIOMED CENTRAL LTD https://epic.awi.de/id/eprint/50796/1/Monteiro_etal_2019_BMCPlantBiology.pdf https://hdl.handle.net/ Monteiro, C. , Li, H. , Bischof, K. , Bartsch, I. orcid:0000-0001-7609-2149 , Valentin, K. U. , Corre, E. , Collén, J. , Harms, L. orcid:0000-0001-7620-0613 , Glöckner, G. and Heinrich, S. (2019) Is geographical variation driving the transcriptomic responses to multiple stressors in the kelp Saccharina latissima , BMC Plant Biology, 19 , p. 513 . doi:10.1186/s12870-019-2124-0 <https://doi.org/10.1186/s12870-019-2124-0> , hdl:10013/epic.1d7c3264-439c-44dc-8237-540bbc7e518f EPIC3BMC Plant Biology, BIOMED CENTRAL LTD, 19, pp. 513, ISSN: 1471-2229 Article isiRev 2019 ftawi https://doi.org/10.1186/s12870-019-2124-0 2021-12-24T15:45:09Z Background: Kelps (Laminariales, Phaeophyceae) are brown macroalgae of utmost ecological, and increasingly economic, importance on temperate to polar rocky shores. Omics approaches in brown algae are still scarce and knowledge of their acclimation mechanisms to the changing conditions experienced in coastal environments can benefit from the application of RNA-sequencing. Despite evidence of ecotypic differentiation, transcriptomic responses from distinct geographical locations have, to our knowledge, never been studied in the sugar kelp Saccharina latissima so far. Results: In this study we investigated gene expression responses using RNA-sequencing of S. latissima from environments with contrasting temperature and salinity conditions – Roscoff, in temperate eastern Atlantic, and Spitsbergen in the Arctic. Juvenile sporophytes derived from uniparental stock cultures from both locations were pre-cultivated at 8 °C and SA 30. Sporophytes acclimated to 0 °C, 8 °C and 15 °C were exposed to a low salinity treatment (SA 20) for 24 h. Hyposalinity had a greater impact at the transcriptomic level than the temperature alone, and its effects were modulated by temperature. Namely, photosynthesis and pigment synthesis were extensively repressed by low salinity at low temperatures. Although some responses were shared among sporophytes from the different sites, marked differences were revealed by principal component analysis, differential expression and GO enrichment. The interaction between low temperature and low salinity drove the largest changes in gene expression in sporophytes from Roscoff while specimens from Spitsbergen required more metabolic adjustment at higher temperatures. Moreover, genes related to cell wall adjustment were differentially expressed between Spitsbergen and Roscoff control samples. Conclusions: Our study reveals interactive effects of temperature and salinity on transcriptomic profiles in S. latissima. Moreover, our data suggest that under identical culture conditions sporophytes from different locations diverge in their transcriptomic responses. This is probably connected to variations in temperature and salinity in their respective environment of origin. The current transcriptomic results support the plastic response pattern in sugar kelp which is a species with several reported ecotypes. Our data provide the baseline for a better understanding of the underlying processes of physiological plasticity and may help in the future to identify strains adapted to specific environments and its genetic control. Article in Journal/Newspaper Arctic Spitsbergen Alfred Wegener Institute for Polar- and Marine Research (AWI): ePIC (electronic Publication Information Center) Arctic BMC Plant Biology 19 1 |
institution |
Open Polar |
collection |
Alfred Wegener Institute for Polar- and Marine Research (AWI): ePIC (electronic Publication Information Center) |
op_collection_id |
ftawi |
language |
unknown |
description |
Background: Kelps (Laminariales, Phaeophyceae) are brown macroalgae of utmost ecological, and increasingly economic, importance on temperate to polar rocky shores. Omics approaches in brown algae are still scarce and knowledge of their acclimation mechanisms to the changing conditions experienced in coastal environments can benefit from the application of RNA-sequencing. Despite evidence of ecotypic differentiation, transcriptomic responses from distinct geographical locations have, to our knowledge, never been studied in the sugar kelp Saccharina latissima so far. Results: In this study we investigated gene expression responses using RNA-sequencing of S. latissima from environments with contrasting temperature and salinity conditions – Roscoff, in temperate eastern Atlantic, and Spitsbergen in the Arctic. Juvenile sporophytes derived from uniparental stock cultures from both locations were pre-cultivated at 8 °C and SA 30. Sporophytes acclimated to 0 °C, 8 °C and 15 °C were exposed to a low salinity treatment (SA 20) for 24 h. Hyposalinity had a greater impact at the transcriptomic level than the temperature alone, and its effects were modulated by temperature. Namely, photosynthesis and pigment synthesis were extensively repressed by low salinity at low temperatures. Although some responses were shared among sporophytes from the different sites, marked differences were revealed by principal component analysis, differential expression and GO enrichment. The interaction between low temperature and low salinity drove the largest changes in gene expression in sporophytes from Roscoff while specimens from Spitsbergen required more metabolic adjustment at higher temperatures. Moreover, genes related to cell wall adjustment were differentially expressed between Spitsbergen and Roscoff control samples. Conclusions: Our study reveals interactive effects of temperature and salinity on transcriptomic profiles in S. latissima. Moreover, our data suggest that under identical culture conditions sporophytes from different locations diverge in their transcriptomic responses. This is probably connected to variations in temperature and salinity in their respective environment of origin. The current transcriptomic results support the plastic response pattern in sugar kelp which is a species with several reported ecotypes. Our data provide the baseline for a better understanding of the underlying processes of physiological plasticity and may help in the future to identify strains adapted to specific environments and its genetic control. |
format |
Article in Journal/Newspaper |
author |
Monteiro, Catia Li, Huiru Bischof, Kai Bartsch, Inka Valentin, Klaus-Ulrich Corre, Erwan Collén, Jonas Harms, Lars Glöckner, Gernot Heinrich, Sandra |
spellingShingle |
Monteiro, Catia Li, Huiru Bischof, Kai Bartsch, Inka Valentin, Klaus-Ulrich Corre, Erwan Collén, Jonas Harms, Lars Glöckner, Gernot Heinrich, Sandra Is geographical variation driving the transcriptomic responses to multiple stressors in the kelp Saccharina latissima |
author_facet |
Monteiro, Catia Li, Huiru Bischof, Kai Bartsch, Inka Valentin, Klaus-Ulrich Corre, Erwan Collén, Jonas Harms, Lars Glöckner, Gernot Heinrich, Sandra |
author_sort |
Monteiro, Catia |
title |
Is geographical variation driving the transcriptomic responses to multiple stressors in the kelp Saccharina latissima |
title_short |
Is geographical variation driving the transcriptomic responses to multiple stressors in the kelp Saccharina latissima |
title_full |
Is geographical variation driving the transcriptomic responses to multiple stressors in the kelp Saccharina latissima |
title_fullStr |
Is geographical variation driving the transcriptomic responses to multiple stressors in the kelp Saccharina latissima |
title_full_unstemmed |
Is geographical variation driving the transcriptomic responses to multiple stressors in the kelp Saccharina latissima |
title_sort |
is geographical variation driving the transcriptomic responses to multiple stressors in the kelp saccharina latissima |
publisher |
BIOMED CENTRAL LTD |
publishDate |
2019 |
url |
https://epic.awi.de/id/eprint/50796/ https://epic.awi.de/id/eprint/50796/1/Monteiro_etal_2019_BMCPlantBiology.pdf https://hdl.handle.net/10013/epic.1d7c3264-439c-44dc-8237-540bbc7e518f https://hdl.handle.net/ |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Spitsbergen |
genre_facet |
Arctic Spitsbergen |
op_source |
EPIC3BMC Plant Biology, BIOMED CENTRAL LTD, 19, pp. 513, ISSN: 1471-2229 |
op_relation |
https://epic.awi.de/id/eprint/50796/1/Monteiro_etal_2019_BMCPlantBiology.pdf https://hdl.handle.net/ Monteiro, C. , Li, H. , Bischof, K. , Bartsch, I. orcid:0000-0001-7609-2149 , Valentin, K. U. , Corre, E. , Collén, J. , Harms, L. orcid:0000-0001-7620-0613 , Glöckner, G. and Heinrich, S. (2019) Is geographical variation driving the transcriptomic responses to multiple stressors in the kelp Saccharina latissima , BMC Plant Biology, 19 , p. 513 . doi:10.1186/s12870-019-2124-0 <https://doi.org/10.1186/s12870-019-2124-0> , hdl:10013/epic.1d7c3264-439c-44dc-8237-540bbc7e518f |
op_doi |
https://doi.org/10.1186/s12870-019-2124-0 |
container_title |
BMC Plant Biology |
container_volume |
19 |
container_issue |
1 |
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1766347886910504960 |