BRIDGING FUNCTIONAL AND PHYLOGENETIC DIVERSITY OF MARINE HETEROTROPHIC PROTISTS VIA SINGLE-CELL TRANSCRIPTOMICS
The comprehensive description of unicellular heterotrophic protists is essential for understanding the functioning of marine ecosystems and defining evolutionary relationships within marine microbial communities. For that reason, new insights into the functional genes of key protists, such as ciliat...
Published in: | European Journal of Phycology |
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European Journal of Phycology
2019
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Online Access: | https://epic.awi.de/id/eprint/50076/ https://epic.awi.de/id/eprint/50076/1/Keynote_and_Oral_Papers.pdf http://epcseven.biol.pmf.hr/programme/ https://hdl.handle.net/10013/epic.11fe5744-bb49-4ae0-bd77-27be4780c4e9 |
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ftawi:oai:epic.awi.de:50076 2024-09-15T18:02:30+00:00 BRIDGING FUNCTIONAL AND PHYLOGENETIC DIVERSITY OF MARINE HETEROTROPHIC PROTISTS VIA SINGLE-CELL TRANSCRIPTOMICS Kalita, Sabrina Edvardsen, Bente Kohli, Gurjeet Janouškovec, Jan Šupraha, Luka Hansen, P. J. John, Uwe 2019-08 application/pdf https://epic.awi.de/id/eprint/50076/ https://epic.awi.de/id/eprint/50076/1/Keynote_and_Oral_Papers.pdf http://epcseven.biol.pmf.hr/programme/ https://hdl.handle.net/10013/epic.11fe5744-bb49-4ae0-bd77-27be4780c4e9 unknown European Journal of Phycology https://epic.awi.de/id/eprint/50076/1/Keynote_and_Oral_Papers.pdf Kalita, S. , Edvardsen, B. , Kohli, G. , Janouškovec, J. , Šupraha, L. , Hansen, P. J. and John, U. orcid:0000-0002-1297-4086 , Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research, University of Oslo, Department of Biosciences, University College London, University of Copenhagen, Department of Biology (2019) BRIDGING FUNCTIONAL AND PHYLOGENETIC DIVERSITY OF MARINE HETEROTROPHIC PROTISTS VIA SINGLE-CELL TRANSCRIPTOMICS , 7th European Phycological Congress, Zagreb, Croatia, 25 August 2019 - 30 August 2019 . doi:10.1080/09670262.2019.1626627 <https://doi.org/10.1080/09670262.2019.1626627> , hdl:10013/epic.11fe5744-bb49-4ae0-bd77-27be4780c4e9 EPIC37th European Phycological Congress, Zagreb, Croatia, 2019-08-25-2019-08-30European Journal of Phycology Conference notRev 2019 ftawi https://doi.org/10.1080/09670262.2019.1626627 2024-06-24T04:22:11Z The comprehensive description of unicellular heterotrophic protists is essential for understanding the functioning of marine ecosystems and defining evolutionary relationships within marine microbial communities. For that reason, new insights into the functional genes of key protists, such as ciliates and dinoflagellates, are needed to complement the increasing taxonomic complexity and bridge the gap between various eco-functional processes in the ocean. In this study, single-cell transcriptomic sequencing proved to be an efficient method to create a snapshot of expressed genes of unicellular heterotrophs. We sequenced 65 single-cell transcriptomes from 20 fresh field samples collected from Sub-Arctic and North Sea waters. These 13 ciliate and 52 dinoflagellate transcriptomes will generally contribute to a greater understanding of functional and evolutionary processes of these marine protists. Further, we generated multi-gene phylogenies of several dozen genes to unravel the relationships of these heterotrophic taxa to other dinoflagellates and ciliates, respectively. These approaches also helped to elucidate the evolution of functional genes and traits for these understudied essential groups. Additionally, the datasets were incorporated into our metatranscriptomic reference database to fill the gap (of approx. 50%) of genomic information of heterotrophic organisms and their functional processes. Overall, identifying the phylogenetic relationships and functional diversity of heterotrophic and mixotrophic protists will clarify paramount marine microbial food web processes and provide clues to the system's sensitivity to climate change. Conference Object Climate change Alfred Wegener Institute for Polar- and Marine Research (AWI): ePIC (electronic Publication Information Center) European Journal of Phycology 54 sup1 31 117 |
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Alfred Wegener Institute for Polar- and Marine Research (AWI): ePIC (electronic Publication Information Center) |
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ftawi |
language |
unknown |
description |
The comprehensive description of unicellular heterotrophic protists is essential for understanding the functioning of marine ecosystems and defining evolutionary relationships within marine microbial communities. For that reason, new insights into the functional genes of key protists, such as ciliates and dinoflagellates, are needed to complement the increasing taxonomic complexity and bridge the gap between various eco-functional processes in the ocean. In this study, single-cell transcriptomic sequencing proved to be an efficient method to create a snapshot of expressed genes of unicellular heterotrophs. We sequenced 65 single-cell transcriptomes from 20 fresh field samples collected from Sub-Arctic and North Sea waters. These 13 ciliate and 52 dinoflagellate transcriptomes will generally contribute to a greater understanding of functional and evolutionary processes of these marine protists. Further, we generated multi-gene phylogenies of several dozen genes to unravel the relationships of these heterotrophic taxa to other dinoflagellates and ciliates, respectively. These approaches also helped to elucidate the evolution of functional genes and traits for these understudied essential groups. Additionally, the datasets were incorporated into our metatranscriptomic reference database to fill the gap (of approx. 50%) of genomic information of heterotrophic organisms and their functional processes. Overall, identifying the phylogenetic relationships and functional diversity of heterotrophic and mixotrophic protists will clarify paramount marine microbial food web processes and provide clues to the system's sensitivity to climate change. |
format |
Conference Object |
author |
Kalita, Sabrina Edvardsen, Bente Kohli, Gurjeet Janouškovec, Jan Šupraha, Luka Hansen, P. J. John, Uwe |
spellingShingle |
Kalita, Sabrina Edvardsen, Bente Kohli, Gurjeet Janouškovec, Jan Šupraha, Luka Hansen, P. J. John, Uwe BRIDGING FUNCTIONAL AND PHYLOGENETIC DIVERSITY OF MARINE HETEROTROPHIC PROTISTS VIA SINGLE-CELL TRANSCRIPTOMICS |
author_facet |
Kalita, Sabrina Edvardsen, Bente Kohli, Gurjeet Janouškovec, Jan Šupraha, Luka Hansen, P. J. John, Uwe |
author_sort |
Kalita, Sabrina |
title |
BRIDGING FUNCTIONAL AND PHYLOGENETIC DIVERSITY OF MARINE HETEROTROPHIC PROTISTS VIA SINGLE-CELL TRANSCRIPTOMICS |
title_short |
BRIDGING FUNCTIONAL AND PHYLOGENETIC DIVERSITY OF MARINE HETEROTROPHIC PROTISTS VIA SINGLE-CELL TRANSCRIPTOMICS |
title_full |
BRIDGING FUNCTIONAL AND PHYLOGENETIC DIVERSITY OF MARINE HETEROTROPHIC PROTISTS VIA SINGLE-CELL TRANSCRIPTOMICS |
title_fullStr |
BRIDGING FUNCTIONAL AND PHYLOGENETIC DIVERSITY OF MARINE HETEROTROPHIC PROTISTS VIA SINGLE-CELL TRANSCRIPTOMICS |
title_full_unstemmed |
BRIDGING FUNCTIONAL AND PHYLOGENETIC DIVERSITY OF MARINE HETEROTROPHIC PROTISTS VIA SINGLE-CELL TRANSCRIPTOMICS |
title_sort |
bridging functional and phylogenetic diversity of marine heterotrophic protists via single-cell transcriptomics |
publisher |
European Journal of Phycology |
publishDate |
2019 |
url |
https://epic.awi.de/id/eprint/50076/ https://epic.awi.de/id/eprint/50076/1/Keynote_and_Oral_Papers.pdf http://epcseven.biol.pmf.hr/programme/ https://hdl.handle.net/10013/epic.11fe5744-bb49-4ae0-bd77-27be4780c4e9 |
genre |
Climate change |
genre_facet |
Climate change |
op_source |
EPIC37th European Phycological Congress, Zagreb, Croatia, 2019-08-25-2019-08-30European Journal of Phycology |
op_relation |
https://epic.awi.de/id/eprint/50076/1/Keynote_and_Oral_Papers.pdf Kalita, S. , Edvardsen, B. , Kohli, G. , Janouškovec, J. , Šupraha, L. , Hansen, P. J. and John, U. orcid:0000-0002-1297-4086 , Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research, University of Oslo, Department of Biosciences, University College London, University of Copenhagen, Department of Biology (2019) BRIDGING FUNCTIONAL AND PHYLOGENETIC DIVERSITY OF MARINE HETEROTROPHIC PROTISTS VIA SINGLE-CELL TRANSCRIPTOMICS , 7th European Phycological Congress, Zagreb, Croatia, 25 August 2019 - 30 August 2019 . doi:10.1080/09670262.2019.1626627 <https://doi.org/10.1080/09670262.2019.1626627> , hdl:10013/epic.11fe5744-bb49-4ae0-bd77-27be4780c4e9 |
op_doi |
https://doi.org/10.1080/09670262.2019.1626627 |
container_title |
European Journal of Phycology |
container_volume |
54 |
container_issue |
sup1 |
container_start_page |
31 |
op_container_end_page |
117 |
_version_ |
1810439952747462656 |