In vivo 31P-MRS of muscle bioenergetics in marine invertebrates: Future ocean limits scallops' performance
Object: Dynamic in vivo 31P-NMR spectroscopy in combination with Magnetic Resonance Imaging (MRI) was used to study muscle bioenergetics of boreal and Arctic scallops (Pecten maximus and Chlamys islandica) to test the hypothesis that future Ocean Warming and Acidification (OWA) will impair the perfo...
Published in: | Magnetic Resonance Imaging |
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Main Authors: | , , , , , , |
Format: | Article in Journal/Newspaper |
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ELSEVIER SCIENCE INC
2019
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Online Access: | https://epic.awi.de/id/eprint/49709/ https://epic.awi.de/id/eprint/49709/1/CB_31P_oxcap_MRI19.pdf https://hdl.handle.net/10013/epic.8846db7c-958c-41e0-8cb9-f09873b53eb5 |
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ftawi:oai:epic.awi.de:49709 2024-09-15T18:02:29+00:00 In vivo 31P-MRS of muscle bioenergetics in marine invertebrates: Future ocean limits scallops' performance Bock, Christian Wermter, Felizitas Schalkhausser, Burgel Blicher, Martin E. Pörtner, Hans-Otto Lannig, Gisela Sejr, Mikael K. 2019-06-12 application/pdf https://epic.awi.de/id/eprint/49709/ https://epic.awi.de/id/eprint/49709/1/CB_31P_oxcap_MRI19.pdf https://hdl.handle.net/10013/epic.8846db7c-958c-41e0-8cb9-f09873b53eb5 unknown ELSEVIER SCIENCE INC https://epic.awi.de/id/eprint/49709/1/CB_31P_oxcap_MRI19.pdf Bock, C. orcid:0000-0003-0052-3090 , Wermter, F. , Schalkhausser, B. , Blicher, M. E. , Pörtner, H. O. orcid:0000-0001-6535-6575 , Lannig, G. orcid:0000-0002-9210-256X and Sejr, M. K. (2019) In vivo 31P-MRS of muscle bioenergetics in marine invertebrates: Future ocean limits scallops' performance , Magnetic Resonance Imaging, 61 , pp. 239-246 . doi:10.1016/j.mri.2019.06.003 <https://doi.org/10.1016/j.mri.2019.06.003> , hdl:10013/epic.8846db7c-958c-41e0-8cb9-f09873b53eb5 EPIC3Magnetic Resonance Imaging, ELSEVIER SCIENCE INC, 61, pp. 239-246, ISSN: 0730-725X Article isiRev 2019 ftawi https://doi.org/10.1016/j.mri.2019.06.003 2024-06-24T04:22:11Z Object: Dynamic in vivo 31P-NMR spectroscopy in combination with Magnetic Resonance Imaging (MRI) was used to study muscle bioenergetics of boreal and Arctic scallops (Pecten maximus and Chlamys islandica) to test the hypothesis that future Ocean Warming and Acidification (OWA) will impair the performance of marine invertebrates. Materials & methods: Experiments were conducted following the recommendations for studies of muscle bioenergetics in vertebrates. Animals were long-term incubated under different environmental conditions: controls at 0 °C for C. islandica and 15 °C for P. maximus under ambient PCO2 of 0.039 kPa, a warm exposure with +5 °C (5 °C and 20 °C, respectively) under ambient PCO2 (OW group), and a combined exposure to warmed acidified conditions (5 °C and 20 °C, 0.112 kPa PCO2, OWA group). Scallops were placed in a 4.7 T MR animal scanner and the energetic status of the adductor muscle was determined under resting conditions using in vivo 31P-NMR spectroscopy. The surplus oxidative flux (Qmax) was quantified by recording the recovery of arginine phosphate (PLA) directly after moderate swimming exercise of the scallops. Results: Measurements led to reproducible results within each experimental group. Under projected future conditions resting PLA levels (PLArest) were reduced, indicating reduced energy reserves in warming exposed scallops per se. In comparison to vertebrate muscle tissue surplus Qmax of scallop muscle was about one order of magnitude lower. This can be explained by lower mitochondrial contents and capacities in invertebrate than vertebrate muscle tissue. Warm exposed scallops showed a slower recovery rate of PLA levels (kPLA) and a reduced surplus Qmax. Elevated PCO2 did not affected PLA recovery further. Conclusion: Dynamic in vivo 31P-NMR spectroscopy revealed constrained residual aerobic power budgets in boreal and Arctic scallops under projected ocean warming and acidification indicating that scallops are susceptible to future climate change. The observed reduction in ... Article in Journal/Newspaper Climate change Alfred Wegener Institute for Polar- and Marine Research (AWI): ePIC (electronic Publication Information Center) Magnetic Resonance Imaging 61 239 246 |
institution |
Open Polar |
collection |
Alfred Wegener Institute for Polar- and Marine Research (AWI): ePIC (electronic Publication Information Center) |
op_collection_id |
ftawi |
language |
unknown |
description |
Object: Dynamic in vivo 31P-NMR spectroscopy in combination with Magnetic Resonance Imaging (MRI) was used to study muscle bioenergetics of boreal and Arctic scallops (Pecten maximus and Chlamys islandica) to test the hypothesis that future Ocean Warming and Acidification (OWA) will impair the performance of marine invertebrates. Materials & methods: Experiments were conducted following the recommendations for studies of muscle bioenergetics in vertebrates. Animals were long-term incubated under different environmental conditions: controls at 0 °C for C. islandica and 15 °C for P. maximus under ambient PCO2 of 0.039 kPa, a warm exposure with +5 °C (5 °C and 20 °C, respectively) under ambient PCO2 (OW group), and a combined exposure to warmed acidified conditions (5 °C and 20 °C, 0.112 kPa PCO2, OWA group). Scallops were placed in a 4.7 T MR animal scanner and the energetic status of the adductor muscle was determined under resting conditions using in vivo 31P-NMR spectroscopy. The surplus oxidative flux (Qmax) was quantified by recording the recovery of arginine phosphate (PLA) directly after moderate swimming exercise of the scallops. Results: Measurements led to reproducible results within each experimental group. Under projected future conditions resting PLA levels (PLArest) were reduced, indicating reduced energy reserves in warming exposed scallops per se. In comparison to vertebrate muscle tissue surplus Qmax of scallop muscle was about one order of magnitude lower. This can be explained by lower mitochondrial contents and capacities in invertebrate than vertebrate muscle tissue. Warm exposed scallops showed a slower recovery rate of PLA levels (kPLA) and a reduced surplus Qmax. Elevated PCO2 did not affected PLA recovery further. Conclusion: Dynamic in vivo 31P-NMR spectroscopy revealed constrained residual aerobic power budgets in boreal and Arctic scallops under projected ocean warming and acidification indicating that scallops are susceptible to future climate change. The observed reduction in ... |
format |
Article in Journal/Newspaper |
author |
Bock, Christian Wermter, Felizitas Schalkhausser, Burgel Blicher, Martin E. Pörtner, Hans-Otto Lannig, Gisela Sejr, Mikael K. |
spellingShingle |
Bock, Christian Wermter, Felizitas Schalkhausser, Burgel Blicher, Martin E. Pörtner, Hans-Otto Lannig, Gisela Sejr, Mikael K. In vivo 31P-MRS of muscle bioenergetics in marine invertebrates: Future ocean limits scallops' performance |
author_facet |
Bock, Christian Wermter, Felizitas Schalkhausser, Burgel Blicher, Martin E. Pörtner, Hans-Otto Lannig, Gisela Sejr, Mikael K. |
author_sort |
Bock, Christian |
title |
In vivo 31P-MRS of muscle bioenergetics in marine invertebrates: Future ocean limits scallops' performance |
title_short |
In vivo 31P-MRS of muscle bioenergetics in marine invertebrates: Future ocean limits scallops' performance |
title_full |
In vivo 31P-MRS of muscle bioenergetics in marine invertebrates: Future ocean limits scallops' performance |
title_fullStr |
In vivo 31P-MRS of muscle bioenergetics in marine invertebrates: Future ocean limits scallops' performance |
title_full_unstemmed |
In vivo 31P-MRS of muscle bioenergetics in marine invertebrates: Future ocean limits scallops' performance |
title_sort |
in vivo 31p-mrs of muscle bioenergetics in marine invertebrates: future ocean limits scallops' performance |
publisher |
ELSEVIER SCIENCE INC |
publishDate |
2019 |
url |
https://epic.awi.de/id/eprint/49709/ https://epic.awi.de/id/eprint/49709/1/CB_31P_oxcap_MRI19.pdf https://hdl.handle.net/10013/epic.8846db7c-958c-41e0-8cb9-f09873b53eb5 |
genre |
Climate change |
genre_facet |
Climate change |
op_source |
EPIC3Magnetic Resonance Imaging, ELSEVIER SCIENCE INC, 61, pp. 239-246, ISSN: 0730-725X |
op_relation |
https://epic.awi.de/id/eprint/49709/1/CB_31P_oxcap_MRI19.pdf Bock, C. orcid:0000-0003-0052-3090 , Wermter, F. , Schalkhausser, B. , Blicher, M. E. , Pörtner, H. O. orcid:0000-0001-6535-6575 , Lannig, G. orcid:0000-0002-9210-256X and Sejr, M. K. (2019) In vivo 31P-MRS of muscle bioenergetics in marine invertebrates: Future ocean limits scallops' performance , Magnetic Resonance Imaging, 61 , pp. 239-246 . doi:10.1016/j.mri.2019.06.003 <https://doi.org/10.1016/j.mri.2019.06.003> , hdl:10013/epic.8846db7c-958c-41e0-8cb9-f09873b53eb5 |
op_doi |
https://doi.org/10.1016/j.mri.2019.06.003 |
container_title |
Magnetic Resonance Imaging |
container_volume |
61 |
container_start_page |
239 |
op_container_end_page |
246 |
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1810439941739511808 |