Pandemic GII.4 Sydney and Epidemic GII.17 Kawasaki308 Noroviruses Display Distinct Specificities for Histo-Blood Group Antigens Leading to Different Transmission Vector Dynamics in Pacific Oysters
Noroviruses are the major cause of foodborne outbreaks of acute gastroenteritis, which are quiet often linked to raw oyster consumption. Previous studies have suggested histo-blood group antigens (HBGA)-like structures in the oyster tissues as ligands for norovirus binding and persistence. To better...
Published in: | Frontiers in Microbiology |
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Online Access: | https://epic.awi.de/id/eprint/48647/ https://epic.awi.de/id/eprint/48647/1/Morozov_2018.pdf https://doi.org/10.3389/fmicb.2018.02826 https://hdl.handle.net/10013/epic.4f70cf56-7f30-4ee9-8654-393732807a53 https://hdl.handle.net/ |
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ftawi:oai:epic.awi.de:48647 2023-05-15T15:59:07+02:00 Pandemic GII.4 Sydney and Epidemic GII.17 Kawasaki308 Noroviruses Display Distinct Specificities for Histo-Blood Group Antigens Leading to Different Transmission Vector Dynamics in Pacific Oysters Morozov, Vasily Hanisch, Franz-Georg Wegner, K. Mathias Schroten, Horst 2018-11-27 application/pdf https://epic.awi.de/id/eprint/48647/ https://epic.awi.de/id/eprint/48647/1/Morozov_2018.pdf https://doi.org/10.3389/fmicb.2018.02826 https://hdl.handle.net/10013/epic.4f70cf56-7f30-4ee9-8654-393732807a53 https://hdl.handle.net/ unknown https://epic.awi.de/id/eprint/48647/1/Morozov_2018.pdf https://hdl.handle.net/ Morozov, V. , Hanisch, F. G. , Wegner, K. M. orcid:0000-0002-2410-8898 and Schroten, H. (2018) Pandemic GII.4 Sydney and Epidemic GII.17 Kawasaki308 Noroviruses Display Distinct Specificities for Histo-Blood Group Antigens Leading to Different Transmission Vector Dynamics in Pacific Oysters , Frontiers in Microbiology, 9 . doi:10.3389/fmicb.2018.02826 <https://doi.org/10.3389/fmicb.2018.02826> , hdl:10013/epic.4f70cf56-7f30-4ee9-8654-393732807a53 EPIC3Frontiers in Microbiology, 9, ISSN: 1664-302X Article isiRev 2018 ftawi https://doi.org/10.3389/fmicb.2018.02826 2021-12-24T15:44:22Z Noroviruses are the major cause of foodborne outbreaks of acute gastroenteritis, which are quiet often linked to raw oyster consumption. Previous studies have suggested histo-blood group antigens (HBGA)-like structures in the oyster tissues as ligands for norovirus binding and persistence. To better understand how oysters can function as vectors for common human noroviruses we have first tested the ability of the GI.1 West Chester, the pandemic GII.4 Sydney, and the epidemic GII.17 Kawasaki308 strains to interact with oyster tissues, and secondly explored how the HBGA preferences of these strains can affect their persistence in oyster tissues. We have found limited HBGA expression in oyster tissues. Only A and H type 1 HBGAs were present in digestive tissues and palps of the Pacific oyster Crassostrea gigas, while gills and mantle lack any HBGA structures. Virus-Like particles (VLPs) of the GI.1 West Chester norovirus reacted with the digestive tissues and palps. Despite of the lack of HBGA expression in mantle, dominant GII.4 Sydney strain readily bound to all the oyster tissues, including digestive tissues, gills, palps, and mantle. In contrast, no binding of the epidemic GII.17 Kawasaki308 VLPs to any oyster tissues was observed. In synthetic HBGA and saliva-binding assays, GI.1 reacted with A type, H type, and Lewis b HBGAs. GII.4 Sydney VLPs showed a broad binding pattern and interacted with various HBGA types, including H type 1 structures. Compared to GI.1 and GII.4 VLPs, the GII.17 Kawasaki308 VLPs only weakly associated with HBGAs carbohydrates and mainly exhibited low affinity binding to long-chain saccharides containing A type, B type, H type, Leb blood group epitopes. Our findings therefore indicate that GI.1 and GII.4 noroviruses are likely to be concentrated in the oysters via HBGA-like glycans potentially leading to increased long term transmission, while for the GII.17 Kawasaki308 strain oysters can only function as short term transmission vectors in periods of high environmental virus concentrations. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Alfred Wegener Institute for Polar- and Marine Research (AWI): ePIC (electronic Publication Information Center) Pacific Frontiers in Microbiology 9 |
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Open Polar |
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Alfred Wegener Institute for Polar- and Marine Research (AWI): ePIC (electronic Publication Information Center) |
op_collection_id |
ftawi |
language |
unknown |
description |
Noroviruses are the major cause of foodborne outbreaks of acute gastroenteritis, which are quiet often linked to raw oyster consumption. Previous studies have suggested histo-blood group antigens (HBGA)-like structures in the oyster tissues as ligands for norovirus binding and persistence. To better understand how oysters can function as vectors for common human noroviruses we have first tested the ability of the GI.1 West Chester, the pandemic GII.4 Sydney, and the epidemic GII.17 Kawasaki308 strains to interact with oyster tissues, and secondly explored how the HBGA preferences of these strains can affect their persistence in oyster tissues. We have found limited HBGA expression in oyster tissues. Only A and H type 1 HBGAs were present in digestive tissues and palps of the Pacific oyster Crassostrea gigas, while gills and mantle lack any HBGA structures. Virus-Like particles (VLPs) of the GI.1 West Chester norovirus reacted with the digestive tissues and palps. Despite of the lack of HBGA expression in mantle, dominant GII.4 Sydney strain readily bound to all the oyster tissues, including digestive tissues, gills, palps, and mantle. In contrast, no binding of the epidemic GII.17 Kawasaki308 VLPs to any oyster tissues was observed. In synthetic HBGA and saliva-binding assays, GI.1 reacted with A type, H type, and Lewis b HBGAs. GII.4 Sydney VLPs showed a broad binding pattern and interacted with various HBGA types, including H type 1 structures. Compared to GI.1 and GII.4 VLPs, the GII.17 Kawasaki308 VLPs only weakly associated with HBGAs carbohydrates and mainly exhibited low affinity binding to long-chain saccharides containing A type, B type, H type, Leb blood group epitopes. Our findings therefore indicate that GI.1 and GII.4 noroviruses are likely to be concentrated in the oysters via HBGA-like glycans potentially leading to increased long term transmission, while for the GII.17 Kawasaki308 strain oysters can only function as short term transmission vectors in periods of high environmental virus concentrations. |
format |
Article in Journal/Newspaper |
author |
Morozov, Vasily Hanisch, Franz-Georg Wegner, K. Mathias Schroten, Horst |
spellingShingle |
Morozov, Vasily Hanisch, Franz-Georg Wegner, K. Mathias Schroten, Horst Pandemic GII.4 Sydney and Epidemic GII.17 Kawasaki308 Noroviruses Display Distinct Specificities for Histo-Blood Group Antigens Leading to Different Transmission Vector Dynamics in Pacific Oysters |
author_facet |
Morozov, Vasily Hanisch, Franz-Georg Wegner, K. Mathias Schroten, Horst |
author_sort |
Morozov, Vasily |
title |
Pandemic GII.4 Sydney and Epidemic GII.17 Kawasaki308 Noroviruses Display Distinct Specificities for Histo-Blood Group Antigens Leading to Different Transmission Vector Dynamics in Pacific Oysters |
title_short |
Pandemic GII.4 Sydney and Epidemic GII.17 Kawasaki308 Noroviruses Display Distinct Specificities for Histo-Blood Group Antigens Leading to Different Transmission Vector Dynamics in Pacific Oysters |
title_full |
Pandemic GII.4 Sydney and Epidemic GII.17 Kawasaki308 Noroviruses Display Distinct Specificities for Histo-Blood Group Antigens Leading to Different Transmission Vector Dynamics in Pacific Oysters |
title_fullStr |
Pandemic GII.4 Sydney and Epidemic GII.17 Kawasaki308 Noroviruses Display Distinct Specificities for Histo-Blood Group Antigens Leading to Different Transmission Vector Dynamics in Pacific Oysters |
title_full_unstemmed |
Pandemic GII.4 Sydney and Epidemic GII.17 Kawasaki308 Noroviruses Display Distinct Specificities for Histo-Blood Group Antigens Leading to Different Transmission Vector Dynamics in Pacific Oysters |
title_sort |
pandemic gii.4 sydney and epidemic gii.17 kawasaki308 noroviruses display distinct specificities for histo-blood group antigens leading to different transmission vector dynamics in pacific oysters |
publishDate |
2018 |
url |
https://epic.awi.de/id/eprint/48647/ https://epic.awi.de/id/eprint/48647/1/Morozov_2018.pdf https://doi.org/10.3389/fmicb.2018.02826 https://hdl.handle.net/10013/epic.4f70cf56-7f30-4ee9-8654-393732807a53 https://hdl.handle.net/ |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
EPIC3Frontiers in Microbiology, 9, ISSN: 1664-302X |
op_relation |
https://epic.awi.de/id/eprint/48647/1/Morozov_2018.pdf https://hdl.handle.net/ Morozov, V. , Hanisch, F. G. , Wegner, K. M. orcid:0000-0002-2410-8898 and Schroten, H. (2018) Pandemic GII.4 Sydney and Epidemic GII.17 Kawasaki308 Noroviruses Display Distinct Specificities for Histo-Blood Group Antigens Leading to Different Transmission Vector Dynamics in Pacific Oysters , Frontiers in Microbiology, 9 . doi:10.3389/fmicb.2018.02826 <https://doi.org/10.3389/fmicb.2018.02826> , hdl:10013/epic.4f70cf56-7f30-4ee9-8654-393732807a53 |
op_doi |
https://doi.org/10.3389/fmicb.2018.02826 |
container_title |
Frontiers in Microbiology |
container_volume |
9 |
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1766394913415495680 |