Bioactivity Screening of Microorganisms Isolated from Arctic Ocean Deep-Sea Sediment
Oceans cover >70% of the earth and encompass variable habitats concerning salinity, temperature, pressure, light availability. The deep sea (>1000 m water depth) constitutes more than 60% of the ocean´s biosphere and harbors an unparalleled biodiversity. It constitutes an extreme habitat due t...
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ftawi:oai:epic.awi.de:48592 2023-05-15T14:27:17+02:00 Bioactivity Screening of Microorganisms Isolated from Arctic Ocean Deep-Sea Sediment Van Soen, Gwendolin Magot, Florent Wenzel-Storjohann, Arlette Blümel, Martina Soltwedel, Thomas Tasdemir, Deniz 2019 https://epic.awi.de/id/eprint/48592/ https://hdl.handle.net/10013/epic.6bfb338a-c822-47ce-971a-4d4304bd8e05 unknown Van Soen, G. , Magot, F. , Wenzel-Storjohann, A. , Blümel, M. , Soltwedel, T. orcid:0000-0002-8214-5937 and Tasdemir, D. (2019) Bioactivity Screening of Microorganisms Isolated from Arctic Ocean Deep-Sea Sediment , BIOPROSP: International Conference on Marine Bioprospecting and Biotechnology, Tromsø, Norway, 11 February 2019 - 13 February 2019 . hdl:10013/epic.6bfb338a-c822-47ce-971a-4d4304bd8e05 EPIC3BIOPROSP: International Conference on Marine Bioprospecting and Biotechnology, Tromsø, Norway, 2019-02-11-2019-02-13 Conference notRev 2019 ftawi 2021-12-24T15:44:22Z Oceans cover >70% of the earth and encompass variable habitats concerning salinity, temperature, pressure, light availability. The deep sea (>1000 m water depth) constitutes more than 60% of the ocean´s biosphere and harbors an unparalleled biodiversity. It constitutes an extreme habitat due to high pressure, darkness and often low nutrient and oxygen concentrations. In order to ensure their survival, microorganisms thriving in such environments have to develop unique metabolic adaptations, thus represent an interesting resource for the discovery of new molecules. However, due to access difficulties to deep-sea habitats and the lack of suitable and affordable sampling techniques, deep-sea microorganisms have remained untapped for their potential in marine biodiscovery. In this study, we obtained deep-sea sediment samples from Arctic Ocean (-2432 m), sampled by an ROV during RV Polarstern expedition 108. Isolation of microorganisms has been performed using two specific media for bacteria and fungi, respectively. Isolates were identified by amplification of the 16S rRNA gene (bacteria) and ITS1-2 region (fungi) followed by Sanger sequencing. In total, 70 bacterial isolates were identified covering four phyla (52 Firmicutes, 1 Actinobacteria, 11 Proteobacteria and 6 Bacteroidetes) and seven fungal strains from two different phyla (6 Ascomycota and 1 Basidiomycota). Selected isolates were cultivated in two different media, followed by solvent (EtOAc) extraction and bioactivity screenings against a panel of clinically relevant microbial pathogens and six cancer cell lines. At 100 µg/mL concentration, three bacterial extracts showed antitumor activity (>70%), whereas 17 exhibited activity (>65%) against methicillin-resistant Staphylococcus aureus (MRSA). Notably, only one fungus showed a cultivation medium dependent-high antifungal activity (>90%), highlighting the impact of culture media on the production of bioactive secondary metabolites. Conference Object Arctic Arctic Arctic Ocean Alfred Wegener Institute for Polar- and Marine Research (AWI): ePIC (electronic Publication Information Center) Arctic Arctic Ocean |
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Alfred Wegener Institute for Polar- and Marine Research (AWI): ePIC (electronic Publication Information Center) |
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description |
Oceans cover >70% of the earth and encompass variable habitats concerning salinity, temperature, pressure, light availability. The deep sea (>1000 m water depth) constitutes more than 60% of the ocean´s biosphere and harbors an unparalleled biodiversity. It constitutes an extreme habitat due to high pressure, darkness and often low nutrient and oxygen concentrations. In order to ensure their survival, microorganisms thriving in such environments have to develop unique metabolic adaptations, thus represent an interesting resource for the discovery of new molecules. However, due to access difficulties to deep-sea habitats and the lack of suitable and affordable sampling techniques, deep-sea microorganisms have remained untapped for their potential in marine biodiscovery. In this study, we obtained deep-sea sediment samples from Arctic Ocean (-2432 m), sampled by an ROV during RV Polarstern expedition 108. Isolation of microorganisms has been performed using two specific media for bacteria and fungi, respectively. Isolates were identified by amplification of the 16S rRNA gene (bacteria) and ITS1-2 region (fungi) followed by Sanger sequencing. In total, 70 bacterial isolates were identified covering four phyla (52 Firmicutes, 1 Actinobacteria, 11 Proteobacteria and 6 Bacteroidetes) and seven fungal strains from two different phyla (6 Ascomycota and 1 Basidiomycota). Selected isolates were cultivated in two different media, followed by solvent (EtOAc) extraction and bioactivity screenings against a panel of clinically relevant microbial pathogens and six cancer cell lines. At 100 µg/mL concentration, three bacterial extracts showed antitumor activity (>70%), whereas 17 exhibited activity (>65%) against methicillin-resistant Staphylococcus aureus (MRSA). Notably, only one fungus showed a cultivation medium dependent-high antifungal activity (>90%), highlighting the impact of culture media on the production of bioactive secondary metabolites. |
format |
Conference Object |
author |
Van Soen, Gwendolin Magot, Florent Wenzel-Storjohann, Arlette Blümel, Martina Soltwedel, Thomas Tasdemir, Deniz |
spellingShingle |
Van Soen, Gwendolin Magot, Florent Wenzel-Storjohann, Arlette Blümel, Martina Soltwedel, Thomas Tasdemir, Deniz Bioactivity Screening of Microorganisms Isolated from Arctic Ocean Deep-Sea Sediment |
author_facet |
Van Soen, Gwendolin Magot, Florent Wenzel-Storjohann, Arlette Blümel, Martina Soltwedel, Thomas Tasdemir, Deniz |
author_sort |
Van Soen, Gwendolin |
title |
Bioactivity Screening of Microorganisms Isolated from Arctic Ocean Deep-Sea Sediment |
title_short |
Bioactivity Screening of Microorganisms Isolated from Arctic Ocean Deep-Sea Sediment |
title_full |
Bioactivity Screening of Microorganisms Isolated from Arctic Ocean Deep-Sea Sediment |
title_fullStr |
Bioactivity Screening of Microorganisms Isolated from Arctic Ocean Deep-Sea Sediment |
title_full_unstemmed |
Bioactivity Screening of Microorganisms Isolated from Arctic Ocean Deep-Sea Sediment |
title_sort |
bioactivity screening of microorganisms isolated from arctic ocean deep-sea sediment |
publishDate |
2019 |
url |
https://epic.awi.de/id/eprint/48592/ https://hdl.handle.net/10013/epic.6bfb338a-c822-47ce-971a-4d4304bd8e05 |
geographic |
Arctic Arctic Ocean |
geographic_facet |
Arctic Arctic Ocean |
genre |
Arctic Arctic Arctic Ocean |
genre_facet |
Arctic Arctic Arctic Ocean |
op_source |
EPIC3BIOPROSP: International Conference on Marine Bioprospecting and Biotechnology, Tromsø, Norway, 2019-02-11-2019-02-13 |
op_relation |
Van Soen, G. , Magot, F. , Wenzel-Storjohann, A. , Blümel, M. , Soltwedel, T. orcid:0000-0002-8214-5937 and Tasdemir, D. (2019) Bioactivity Screening of Microorganisms Isolated from Arctic Ocean Deep-Sea Sediment , BIOPROSP: International Conference on Marine Bioprospecting and Biotechnology, Tromsø, Norway, 11 February 2019 - 13 February 2019 . hdl:10013/epic.6bfb338a-c822-47ce-971a-4d4304bd8e05 |
_version_ |
1766300923073658880 |