The Response of Three Southern Ocean Phytoplankton Species to Ocean Acidification and Light Availability: A Transcriptomic Study
Ocean acidification (OA) and high light was found to negatively affect the Antarctic key species Phaeocystis antarctica, Fragilariopsis kerguelensis and Chaetoceros debilis. To unravel the underlying physiological response at the transcriptomic level, these species were grown under ambient and eleva...
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ftawi:oai:epic.awi.de:48226 2024-09-15T17:43:44+00:00 The Response of Three Southern Ocean Phytoplankton Species to Ocean Acidification and Light Availability: A Transcriptomic Study Beszteri, Sara Thoms, Silke Benes, Vladimir Harms, Lars Trimborn, Scarlett 2018 https://epic.awi.de/id/eprint/48226/ https://hdl.handle.net/10013/epic.53fef03f-f3d7-4876-bc2c-cdc6ae4fa8c3 unknown ELSEVIER GMBH Beszteri, S. , Thoms, S. , Benes, V. , Harms, L. orcid:0000-0001-7620-0613 and Trimborn, S. orcid:0000-0003-1434-9927 (2018) The Response of Three Southern Ocean Phytoplankton Species to Ocean Acidification and Light Availability: A Transcriptomic Study , Protist . doi:10.1016/j.protis.2018.08.003 <https://doi.org/10.1016/j.protis.2018.08.003> , hdl:10013/epic.53fef03f-f3d7-4876-bc2c-cdc6ae4fa8c3 EPIC3Protist, ELSEVIER GMBH, ISSN: 1434-4610 Article isiRev 2018 ftawi https://doi.org/10.1016/j.protis.2018.08.003 2024-06-24T04:21:00Z Ocean acidification (OA) and high light was found to negatively affect the Antarctic key species Phaeocystis antarctica, Fragilariopsis kerguelensis and Chaetoceros debilis. To unravel the underlying physiological response at the transcriptomic level, these species were grown under ambient and elevated pCO2 combined with low or high light. RNA sequencing revealed that the haptophyte was much more tolerant towards OA than the two diatoms as only these showed distinct OA-dependent gene regulation patterns. Under ambient pCO2, high light resulted in decreased glycolysis in P. antarctica. Contrastingly, upregulation of genes related to cell division and transcription as well as reduced expression of both cata- and anabolic carbon related pathways were seen in C. debilis. OA in combination with low light led to reduced respiration, but also surprisingly to higher expression of genes involved in light protection, transcription and translation in C. debilis. Though not affecting P. antarctica, OA combined with high light caused also photosensitivity in both diatoms. As additional response reallocation of carbon to lipids was found in C. debilis under these conditions. Overall, we conclude that P. antarctica is better adapted than the two diatoms to OA and high light. Article in Journal/Newspaper Antarc* Antarctic Antarctica Ocean acidification Southern Ocean Alfred Wegener Institute for Polar- and Marine Research (AWI): ePIC (electronic Publication Information Center) Protist 169 6 958 975 |
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Alfred Wegener Institute for Polar- and Marine Research (AWI): ePIC (electronic Publication Information Center) |
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Ocean acidification (OA) and high light was found to negatively affect the Antarctic key species Phaeocystis antarctica, Fragilariopsis kerguelensis and Chaetoceros debilis. To unravel the underlying physiological response at the transcriptomic level, these species were grown under ambient and elevated pCO2 combined with low or high light. RNA sequencing revealed that the haptophyte was much more tolerant towards OA than the two diatoms as only these showed distinct OA-dependent gene regulation patterns. Under ambient pCO2, high light resulted in decreased glycolysis in P. antarctica. Contrastingly, upregulation of genes related to cell division and transcription as well as reduced expression of both cata- and anabolic carbon related pathways were seen in C. debilis. OA in combination with low light led to reduced respiration, but also surprisingly to higher expression of genes involved in light protection, transcription and translation in C. debilis. Though not affecting P. antarctica, OA combined with high light caused also photosensitivity in both diatoms. As additional response reallocation of carbon to lipids was found in C. debilis under these conditions. Overall, we conclude that P. antarctica is better adapted than the two diatoms to OA and high light. |
format |
Article in Journal/Newspaper |
author |
Beszteri, Sara Thoms, Silke Benes, Vladimir Harms, Lars Trimborn, Scarlett |
spellingShingle |
Beszteri, Sara Thoms, Silke Benes, Vladimir Harms, Lars Trimborn, Scarlett The Response of Three Southern Ocean Phytoplankton Species to Ocean Acidification and Light Availability: A Transcriptomic Study |
author_facet |
Beszteri, Sara Thoms, Silke Benes, Vladimir Harms, Lars Trimborn, Scarlett |
author_sort |
Beszteri, Sara |
title |
The Response of Three Southern Ocean Phytoplankton Species to Ocean Acidification and Light Availability: A Transcriptomic Study |
title_short |
The Response of Three Southern Ocean Phytoplankton Species to Ocean Acidification and Light Availability: A Transcriptomic Study |
title_full |
The Response of Three Southern Ocean Phytoplankton Species to Ocean Acidification and Light Availability: A Transcriptomic Study |
title_fullStr |
The Response of Three Southern Ocean Phytoplankton Species to Ocean Acidification and Light Availability: A Transcriptomic Study |
title_full_unstemmed |
The Response of Three Southern Ocean Phytoplankton Species to Ocean Acidification and Light Availability: A Transcriptomic Study |
title_sort |
response of three southern ocean phytoplankton species to ocean acidification and light availability: a transcriptomic study |
publisher |
ELSEVIER GMBH |
publishDate |
2018 |
url |
https://epic.awi.de/id/eprint/48226/ https://hdl.handle.net/10013/epic.53fef03f-f3d7-4876-bc2c-cdc6ae4fa8c3 |
genre |
Antarc* Antarctic Antarctica Ocean acidification Southern Ocean |
genre_facet |
Antarc* Antarctic Antarctica Ocean acidification Southern Ocean |
op_source |
EPIC3Protist, ELSEVIER GMBH, ISSN: 1434-4610 |
op_relation |
Beszteri, S. , Thoms, S. , Benes, V. , Harms, L. orcid:0000-0001-7620-0613 and Trimborn, S. orcid:0000-0003-1434-9927 (2018) The Response of Three Southern Ocean Phytoplankton Species to Ocean Acidification and Light Availability: A Transcriptomic Study , Protist . doi:10.1016/j.protis.2018.08.003 <https://doi.org/10.1016/j.protis.2018.08.003> , hdl:10013/epic.53fef03f-f3d7-4876-bc2c-cdc6ae4fa8c3 |
op_doi |
https://doi.org/10.1016/j.protis.2018.08.003 |
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Protist |
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169 |
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6 |
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958 |
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975 |
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