Calanus finmarchicus seasonal cycle and diapause in relation to gene expression, physiology, and endogenous clocks

The copepod Calanus finmarchicus plays a crucial role in the north Atlantic food web. Its seasonal life cycle involves reproduction and development in surface waters before overwintering in diapause at depth. Although diapause has been studied for more than a century, the factors responsible for the...

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Published in:Limnology and Oceanography
Main Authors: Häfker, N Sören, Teschke, Mathias, Last, Kim S., Pond, David W., Hüppe, Lukas, Meyer, Bettina
Format: Article in Journal/Newspaper
Language:unknown
Published: AMER SOC LIMNOLOGY OCEANOGRAPHY 2018
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Online Access:https://epic.awi.de/id/eprint/48125/
https://epic.awi.de/id/eprint/48125/1/Haefker_et_al_2018a.pdf
https://hdl.handle.net/10013/epic.39038194-fbff-4c9e-b886-c09dd4074062
id ftawi:oai:epic.awi.de:48125
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spelling ftawi:oai:epic.awi.de:48125 2024-06-09T07:45:13+00:00 Calanus finmarchicus seasonal cycle and diapause in relation to gene expression, physiology, and endogenous clocks Häfker, N Sören Teschke, Mathias Last, Kim S. Pond, David W. Hüppe, Lukas Meyer, Bettina 2018-09-04 application/pdf https://epic.awi.de/id/eprint/48125/ https://epic.awi.de/id/eprint/48125/1/Haefker_et_al_2018a.pdf https://hdl.handle.net/10013/epic.39038194-fbff-4c9e-b886-c09dd4074062 unknown AMER SOC LIMNOLOGY OCEANOGRAPHY https://epic.awi.de/id/eprint/48125/1/Haefker_et_al_2018a.pdf Häfker, N. S. orcid:0000-0002-4883-3656 , Teschke, M. , Last, K. S. , Pond, D. W. , Hüppe, L. and Meyer, B. orcid:0000-0001-6804-9896 (2018) Calanus finmarchicus seasonal cycle and diapause in relation to gene expression, physiology, and endogenous clocks , Limnology and Oceanography . doi:10.1002/lno.11011 <https://doi.org/10.1002/lno.11011> , hdl:10013/epic.39038194-fbff-4c9e-b886-c09dd4074062 EPIC3Limnology and Oceanography, AMER SOC LIMNOLOGY OCEANOGRAPHY, ISSN: 0024-3590 Article isiRev 2018 ftawi https://doi.org/10.1002/lno.11011 2024-05-14T23:31:42Z The copepod Calanus finmarchicus plays a crucial role in the north Atlantic food web. Its seasonal life cycle involves reproduction and development in surface waters before overwintering in diapause at depth. Although diapause has been studied for more than a century, the factors responsible for the initiation and termination of it are still unclear. Endogenous clocks have been identified as potent tools for photoperiod measurement and seasonal rhythmicity in many terrestrial species, but knowledge of these remains scarce in the marine realm. Focusing on the dominant CV copepodid stage, we sampled a population of C. finmarchicus from a Scottish sea loch to characterize population dynamics, several physiological parameters, and diel and seasonal expression rhythms of 35 genes representing different metabolic pathways, including the circadian clock machinery. This generated a detailed overview of the seasonal cycle of C. finmarchicus including the most extensive field dataset on circadian clock gene expression in a marine species to date. Gene expression patterns revealed distinct gene clusters upregulated at different phases of the copepod’s seasonal cycle. While diel clock cycling was restricted to the active spring/summer phase, many clock genes exhibited the highest expression during diapause. Our results provide new insights into diapause on physiological and genetic levels. We suggest that photoperiod, in interaction with internal and external factors (lipid content, temperature, food availability) and the endogenous clock mechanism, plays an important role in the timing of diapause in C. finmarchicus. Article in Journal/Newspaper Calanus finmarchicus North Atlantic Alfred Wegener Institute for Polar- and Marine Research (AWI): ePIC (electronic Publication Information Center) Limnology and Oceanography 63 6 2815 2838
institution Open Polar
collection Alfred Wegener Institute for Polar- and Marine Research (AWI): ePIC (electronic Publication Information Center)
op_collection_id ftawi
language unknown
description The copepod Calanus finmarchicus plays a crucial role in the north Atlantic food web. Its seasonal life cycle involves reproduction and development in surface waters before overwintering in diapause at depth. Although diapause has been studied for more than a century, the factors responsible for the initiation and termination of it are still unclear. Endogenous clocks have been identified as potent tools for photoperiod measurement and seasonal rhythmicity in many terrestrial species, but knowledge of these remains scarce in the marine realm. Focusing on the dominant CV copepodid stage, we sampled a population of C. finmarchicus from a Scottish sea loch to characterize population dynamics, several physiological parameters, and diel and seasonal expression rhythms of 35 genes representing different metabolic pathways, including the circadian clock machinery. This generated a detailed overview of the seasonal cycle of C. finmarchicus including the most extensive field dataset on circadian clock gene expression in a marine species to date. Gene expression patterns revealed distinct gene clusters upregulated at different phases of the copepod’s seasonal cycle. While diel clock cycling was restricted to the active spring/summer phase, many clock genes exhibited the highest expression during diapause. Our results provide new insights into diapause on physiological and genetic levels. We suggest that photoperiod, in interaction with internal and external factors (lipid content, temperature, food availability) and the endogenous clock mechanism, plays an important role in the timing of diapause in C. finmarchicus.
format Article in Journal/Newspaper
author Häfker, N Sören
Teschke, Mathias
Last, Kim S.
Pond, David W.
Hüppe, Lukas
Meyer, Bettina
spellingShingle Häfker, N Sören
Teschke, Mathias
Last, Kim S.
Pond, David W.
Hüppe, Lukas
Meyer, Bettina
Calanus finmarchicus seasonal cycle and diapause in relation to gene expression, physiology, and endogenous clocks
author_facet Häfker, N Sören
Teschke, Mathias
Last, Kim S.
Pond, David W.
Hüppe, Lukas
Meyer, Bettina
author_sort Häfker, N Sören
title Calanus finmarchicus seasonal cycle and diapause in relation to gene expression, physiology, and endogenous clocks
title_short Calanus finmarchicus seasonal cycle and diapause in relation to gene expression, physiology, and endogenous clocks
title_full Calanus finmarchicus seasonal cycle and diapause in relation to gene expression, physiology, and endogenous clocks
title_fullStr Calanus finmarchicus seasonal cycle and diapause in relation to gene expression, physiology, and endogenous clocks
title_full_unstemmed Calanus finmarchicus seasonal cycle and diapause in relation to gene expression, physiology, and endogenous clocks
title_sort calanus finmarchicus seasonal cycle and diapause in relation to gene expression, physiology, and endogenous clocks
publisher AMER SOC LIMNOLOGY OCEANOGRAPHY
publishDate 2018
url https://epic.awi.de/id/eprint/48125/
https://epic.awi.de/id/eprint/48125/1/Haefker_et_al_2018a.pdf
https://hdl.handle.net/10013/epic.39038194-fbff-4c9e-b886-c09dd4074062
genre Calanus finmarchicus
North Atlantic
genre_facet Calanus finmarchicus
North Atlantic
op_source EPIC3Limnology and Oceanography, AMER SOC LIMNOLOGY OCEANOGRAPHY, ISSN: 0024-3590
op_relation https://epic.awi.de/id/eprint/48125/1/Haefker_et_al_2018a.pdf
Häfker, N. S. orcid:0000-0002-4883-3656 , Teschke, M. , Last, K. S. , Pond, D. W. , Hüppe, L. and Meyer, B. orcid:0000-0001-6804-9896 (2018) Calanus finmarchicus seasonal cycle and diapause in relation to gene expression, physiology, and endogenous clocks , Limnology and Oceanography . doi:10.1002/lno.11011 <https://doi.org/10.1002/lno.11011> , hdl:10013/epic.39038194-fbff-4c9e-b886-c09dd4074062
op_doi https://doi.org/10.1002/lno.11011
container_title Limnology and Oceanography
container_volume 63
container_issue 6
container_start_page 2815
op_container_end_page 2838
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