Growth suppression of ice crystal basal face in the presence of a moderate ice-binding protein does not confer hyperactivity
Ice-binding proteins (IBPs) affect ice crystal growth by attaching to crystal faces. We present the effects on the growth of an ice single crystal caused by an ice-binding protein from the sea ice microalga Fragilariopsis cylindrus (fcIBP) that is characterized by the widespread domain of unknown fu...
Published in: | Proceedings of the National Academy of Sciences |
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NATL ACAD SCIENCES
2018
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Online Access: | https://epic.awi.de/id/eprint/47622/ https://epic.awi.de/id/eprint/47622/1/PNASMBayerGiraldi2018.pdf https://epic.awi.de/id/eprint/47622/2/SI.pdf https://hdl.handle.net/10013/epic.d4b369ff-8ee8-4baf-b690-ce1a7675cf86 |
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ftawi:oai:epic.awi.de:47622 2024-09-15T18:35:30+00:00 Growth suppression of ice crystal basal face in the presence of a moderate ice-binding protein does not confer hyperactivity Bayer-Giraldi, Maddalena Sazaki, Gen Nagashima, Ken Kipfstuhl, Sepp Vorontsov, Dmitry A. Furukawa, Yoshinori 2018-07-17 application/pdf https://epic.awi.de/id/eprint/47622/ https://epic.awi.de/id/eprint/47622/1/PNASMBayerGiraldi2018.pdf https://epic.awi.de/id/eprint/47622/2/SI.pdf https://hdl.handle.net/10013/epic.d4b369ff-8ee8-4baf-b690-ce1a7675cf86 unknown NATL ACAD SCIENCES https://epic.awi.de/id/eprint/47622/1/PNASMBayerGiraldi2018.pdf https://epic.awi.de/id/eprint/47622/2/SI.pdf Bayer-Giraldi, M. orcid:0000-0002-7158-5478 , Sazaki, G. , Nagashima, K. , Kipfstuhl, S. , Vorontsov, D. A. and Furukawa, Y. (2018) Growth suppression of ice crystal basal face in the presence of a moderate ice-binding protein does not confer hyperactivity , Proceedings of the National Academy of Sciences of the United States of America, 115 (29), pp. 7479-7484 . doi:10.1073/pnas.1807461115 <https://doi.org/10.1073/pnas.1807461115> , hdl:10013/epic.d4b369ff-8ee8-4baf-b690-ce1a7675cf86 EPIC3Proceedings of the National Academy of Sciences of the United States of America, NATL ACAD SCIENCES, 115(29), pp. 7479-7484, ISSN: 0027-8424 Article isiRev 2018 ftawi https://doi.org/10.1073/pnas.1807461115 2024-06-24T04:19:47Z Ice-binding proteins (IBPs) affect ice crystal growth by attaching to crystal faces. We present the effects on the growth of an ice single crystal caused by an ice-binding protein from the sea ice microalga Fragilariopsis cylindrus (fcIBP) that is characterized by the widespread domain of unknown function 3494 (DUF3494) and known to cause a moderate freezing point depression (below 1 °C). By the application of interferometry, bright-field microscopy, and fluorescence microscopy, we observed that the fcIBP attaches to the basal faces of ice crystals, thereby inhibiting their growth in the c direction and resulting in an increase in the effective supercooling with increasing fcIBP concentration. In addition, we observed that the fcIBP attaches to prism faces and inhibits their growth. In the event that the effective supercooling is small and crystals are faceted, this process causes an emergence of prism faces and suppresses crystal growth in the a direction. When the effective supercooling is large and ice crystals have developed into a dendritic shape, the suppression of prism face growth results in thinner dendrite branches, and growth in the a direction is accelerated due to enhanced latent heat dissipation. Our observations clearly indicate that the fcIBP occupies a separate position in the classification of IBPs due to the fact that it suppresses the growth of basal faces, despite its moderate freezing point depression. Article in Journal/Newspaper Sea ice Alfred Wegener Institute for Polar- and Marine Research (AWI): ePIC (electronic Publication Information Center) Proceedings of the National Academy of Sciences 115 29 7479 7484 |
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Open Polar |
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Alfred Wegener Institute for Polar- and Marine Research (AWI): ePIC (electronic Publication Information Center) |
op_collection_id |
ftawi |
language |
unknown |
description |
Ice-binding proteins (IBPs) affect ice crystal growth by attaching to crystal faces. We present the effects on the growth of an ice single crystal caused by an ice-binding protein from the sea ice microalga Fragilariopsis cylindrus (fcIBP) that is characterized by the widespread domain of unknown function 3494 (DUF3494) and known to cause a moderate freezing point depression (below 1 °C). By the application of interferometry, bright-field microscopy, and fluorescence microscopy, we observed that the fcIBP attaches to the basal faces of ice crystals, thereby inhibiting their growth in the c direction and resulting in an increase in the effective supercooling with increasing fcIBP concentration. In addition, we observed that the fcIBP attaches to prism faces and inhibits their growth. In the event that the effective supercooling is small and crystals are faceted, this process causes an emergence of prism faces and suppresses crystal growth in the a direction. When the effective supercooling is large and ice crystals have developed into a dendritic shape, the suppression of prism face growth results in thinner dendrite branches, and growth in the a direction is accelerated due to enhanced latent heat dissipation. Our observations clearly indicate that the fcIBP occupies a separate position in the classification of IBPs due to the fact that it suppresses the growth of basal faces, despite its moderate freezing point depression. |
format |
Article in Journal/Newspaper |
author |
Bayer-Giraldi, Maddalena Sazaki, Gen Nagashima, Ken Kipfstuhl, Sepp Vorontsov, Dmitry A. Furukawa, Yoshinori |
spellingShingle |
Bayer-Giraldi, Maddalena Sazaki, Gen Nagashima, Ken Kipfstuhl, Sepp Vorontsov, Dmitry A. Furukawa, Yoshinori Growth suppression of ice crystal basal face in the presence of a moderate ice-binding protein does not confer hyperactivity |
author_facet |
Bayer-Giraldi, Maddalena Sazaki, Gen Nagashima, Ken Kipfstuhl, Sepp Vorontsov, Dmitry A. Furukawa, Yoshinori |
author_sort |
Bayer-Giraldi, Maddalena |
title |
Growth suppression of ice crystal basal face in the presence of a moderate ice-binding protein does not confer hyperactivity |
title_short |
Growth suppression of ice crystal basal face in the presence of a moderate ice-binding protein does not confer hyperactivity |
title_full |
Growth suppression of ice crystal basal face in the presence of a moderate ice-binding protein does not confer hyperactivity |
title_fullStr |
Growth suppression of ice crystal basal face in the presence of a moderate ice-binding protein does not confer hyperactivity |
title_full_unstemmed |
Growth suppression of ice crystal basal face in the presence of a moderate ice-binding protein does not confer hyperactivity |
title_sort |
growth suppression of ice crystal basal face in the presence of a moderate ice-binding protein does not confer hyperactivity |
publisher |
NATL ACAD SCIENCES |
publishDate |
2018 |
url |
https://epic.awi.de/id/eprint/47622/ https://epic.awi.de/id/eprint/47622/1/PNASMBayerGiraldi2018.pdf https://epic.awi.de/id/eprint/47622/2/SI.pdf https://hdl.handle.net/10013/epic.d4b369ff-8ee8-4baf-b690-ce1a7675cf86 |
genre |
Sea ice |
genre_facet |
Sea ice |
op_source |
EPIC3Proceedings of the National Academy of Sciences of the United States of America, NATL ACAD SCIENCES, 115(29), pp. 7479-7484, ISSN: 0027-8424 |
op_relation |
https://epic.awi.de/id/eprint/47622/1/PNASMBayerGiraldi2018.pdf https://epic.awi.de/id/eprint/47622/2/SI.pdf Bayer-Giraldi, M. orcid:0000-0002-7158-5478 , Sazaki, G. , Nagashima, K. , Kipfstuhl, S. , Vorontsov, D. A. and Furukawa, Y. (2018) Growth suppression of ice crystal basal face in the presence of a moderate ice-binding protein does not confer hyperactivity , Proceedings of the National Academy of Sciences of the United States of America, 115 (29), pp. 7479-7484 . doi:10.1073/pnas.1807461115 <https://doi.org/10.1073/pnas.1807461115> , hdl:10013/epic.d4b369ff-8ee8-4baf-b690-ce1a7675cf86 |
op_doi |
https://doi.org/10.1073/pnas.1807461115 |
container_title |
Proceedings of the National Academy of Sciences |
container_volume |
115 |
container_issue |
29 |
container_start_page |
7479 |
op_container_end_page |
7484 |
_version_ |
1810478693736251392 |