Anaerobic methanotrophic communities thrive in deep submarine permafrost
Thawing submarine permafrost is a source of methane to the subsurface biosphere. Methane oxidation in submarine permafrost sediments has been proposed, but the responsible microorganisms remain uncharacterized. We analyzed archaeal communities and identified distinct anaerobic methanotrophic assembl...
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Online Access: | https://epic.awi.de/id/eprint/47271/ https://epic.awi.de/id/eprint/47271/1/s41598-018-19505-9.pdf https://hdl.handle.net/10013/epic.50662d3b-26f5-4733-bc3c-a7cbe921ef74 |
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ftawi:oai:epic.awi.de:47271 2024-09-15T18:29:20+00:00 Anaerobic methanotrophic communities thrive in deep submarine permafrost Winkel, Matthias Mitzscherling, Julia Overduin, Paul Horn, F Winterfeld, Maria Rijkers, R Grigoriev, M. N. Knoblauch, Christian Mangelsdorf, Kai Wagner, Dirk Liebner, Susanne 2018-01-22 application/pdf https://epic.awi.de/id/eprint/47271/ https://epic.awi.de/id/eprint/47271/1/s41598-018-19505-9.pdf https://hdl.handle.net/10013/epic.50662d3b-26f5-4733-bc3c-a7cbe921ef74 unknown https://epic.awi.de/id/eprint/47271/1/s41598-018-19505-9.pdf Winkel, M. , Mitzscherling, J. , Overduin, P. orcid:0000-0001-9849-4712 , Horn, F. , Winterfeld, M. , Rijkers, R. , Grigoriev, M. N. , Knoblauch, C. , Mangelsdorf, K. , Wagner, D. and Liebner, S. (2018) Anaerobic methanotrophic communities thrive in deep submarine permafrost , Scientific Reports, 8 . doi:10.1038/s41598-018-19505-9 <https://doi.org/10.1038/s41598-018-19505-9> , hdl:10013/epic.50662d3b-26f5-4733-bc3c-a7cbe921ef74 EPIC3Scientific Reports, 8 Article isiRev 2018 ftawi https://doi.org/10.1038/s41598-018-19505-9 2024-06-24T04:19:47Z Thawing submarine permafrost is a source of methane to the subsurface biosphere. Methane oxidation in submarine permafrost sediments has been proposed, but the responsible microorganisms remain uncharacterized. We analyzed archaeal communities and identified distinct anaerobic methanotrophic assemblages of marine and terrestrial origin (ANME-2a/b, ANME-2d) both in frozen and completely thawed submarine permafrost sediments. Besides archaea potentially involved in anaerobic oxidation of methane (AOM) we found a large diversity of archaea mainly belonging to Bathyarchaeota, Thaumarchaeota, and Euryarchaeota. Methane concentrations and δ13C-methane signatures distinguish horizons of potential AOM coupled either to sulfate reduction in a sulfate-methane transition zone (SMTZ) or to the reduction of other electron acceptors, such as iron, manganese or nitrate. Analysis of functional marker genes (mcrA) and fluorescence in situ hybridization (FISH) corroborate potential activity of AOM communities in submarine permafrost sediments at low temperatures. Modeled potential AOM consumes 72–100% of submarine permafrost methane and up to 1.2 Tg of carbon per year for the total expected area of submarine permafrost. This is comparable with AOM habitats such as cold seeps. We thus propose that AOM is active where submarine permafrost thaws, which should be included in global methane budgets. Article in Journal/Newspaper permafrost Alfred Wegener Institute for Polar- and Marine Research (AWI): ePIC (electronic Publication Information Center) Scientific Reports 8 1 |
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Open Polar |
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Alfred Wegener Institute for Polar- and Marine Research (AWI): ePIC (electronic Publication Information Center) |
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ftawi |
language |
unknown |
description |
Thawing submarine permafrost is a source of methane to the subsurface biosphere. Methane oxidation in submarine permafrost sediments has been proposed, but the responsible microorganisms remain uncharacterized. We analyzed archaeal communities and identified distinct anaerobic methanotrophic assemblages of marine and terrestrial origin (ANME-2a/b, ANME-2d) both in frozen and completely thawed submarine permafrost sediments. Besides archaea potentially involved in anaerobic oxidation of methane (AOM) we found a large diversity of archaea mainly belonging to Bathyarchaeota, Thaumarchaeota, and Euryarchaeota. Methane concentrations and δ13C-methane signatures distinguish horizons of potential AOM coupled either to sulfate reduction in a sulfate-methane transition zone (SMTZ) or to the reduction of other electron acceptors, such as iron, manganese or nitrate. Analysis of functional marker genes (mcrA) and fluorescence in situ hybridization (FISH) corroborate potential activity of AOM communities in submarine permafrost sediments at low temperatures. Modeled potential AOM consumes 72–100% of submarine permafrost methane and up to 1.2 Tg of carbon per year for the total expected area of submarine permafrost. This is comparable with AOM habitats such as cold seeps. We thus propose that AOM is active where submarine permafrost thaws, which should be included in global methane budgets. |
format |
Article in Journal/Newspaper |
author |
Winkel, Matthias Mitzscherling, Julia Overduin, Paul Horn, F Winterfeld, Maria Rijkers, R Grigoriev, M. N. Knoblauch, Christian Mangelsdorf, Kai Wagner, Dirk Liebner, Susanne |
spellingShingle |
Winkel, Matthias Mitzscherling, Julia Overduin, Paul Horn, F Winterfeld, Maria Rijkers, R Grigoriev, M. N. Knoblauch, Christian Mangelsdorf, Kai Wagner, Dirk Liebner, Susanne Anaerobic methanotrophic communities thrive in deep submarine permafrost |
author_facet |
Winkel, Matthias Mitzscherling, Julia Overduin, Paul Horn, F Winterfeld, Maria Rijkers, R Grigoriev, M. N. Knoblauch, Christian Mangelsdorf, Kai Wagner, Dirk Liebner, Susanne |
author_sort |
Winkel, Matthias |
title |
Anaerobic methanotrophic communities thrive in deep submarine permafrost |
title_short |
Anaerobic methanotrophic communities thrive in deep submarine permafrost |
title_full |
Anaerobic methanotrophic communities thrive in deep submarine permafrost |
title_fullStr |
Anaerobic methanotrophic communities thrive in deep submarine permafrost |
title_full_unstemmed |
Anaerobic methanotrophic communities thrive in deep submarine permafrost |
title_sort |
anaerobic methanotrophic communities thrive in deep submarine permafrost |
publishDate |
2018 |
url |
https://epic.awi.de/id/eprint/47271/ https://epic.awi.de/id/eprint/47271/1/s41598-018-19505-9.pdf https://hdl.handle.net/10013/epic.50662d3b-26f5-4733-bc3c-a7cbe921ef74 |
genre |
permafrost |
genre_facet |
permafrost |
op_source |
EPIC3Scientific Reports, 8 |
op_relation |
https://epic.awi.de/id/eprint/47271/1/s41598-018-19505-9.pdf Winkel, M. , Mitzscherling, J. , Overduin, P. orcid:0000-0001-9849-4712 , Horn, F. , Winterfeld, M. , Rijkers, R. , Grigoriev, M. N. , Knoblauch, C. , Mangelsdorf, K. , Wagner, D. and Liebner, S. (2018) Anaerobic methanotrophic communities thrive in deep submarine permafrost , Scientific Reports, 8 . doi:10.1038/s41598-018-19505-9 <https://doi.org/10.1038/s41598-018-19505-9> , hdl:10013/epic.50662d3b-26f5-4733-bc3c-a7cbe921ef74 |
op_doi |
https://doi.org/10.1038/s41598-018-19505-9 |
container_title |
Scientific Reports |
container_volume |
8 |
container_issue |
1 |
_version_ |
1810470744994349056 |