A comparison of sedimentary DNA and pollen from lake sediments in recording vegetation composition at the Siberian treeline
Reliable information on past and present vegetation is important to project future changes, especially for rapidly transitioning areas such as the boreal treeline. To study past vegetation, pollen analysis is common, while current vegetation is usually assessed by field surveys. Application of detai...
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ftawi:oai:epic.awi.de:44830 2024-09-15T18:38:49+00:00 A comparison of sedimentary DNA and pollen from lake sediments in recording vegetation composition at the Siberian treeline Niemeyer, Bastian Epp, Laura Stoof-Leichsenring, Kathleen Pestryakova, L. A. Herzschuh, Ulrike 2017 https://epic.awi.de/id/eprint/44830/ https://hdl.handle.net/10013/epic.51073 unknown WILEY-BLACKWELL PUBLISHING Niemeyer, B. , Epp, L. , Stoof-Leichsenring, K. orcid:0000-0002-6609-3217 , Pestryakova, L. A. and Herzschuh, U. orcid:0000-0003-0999-1261 (2017) A comparison of sedimentary DNA and pollen from lake sediments in recording vegetation composition at the Siberian treeline , Molecular Ecology Resources . doi:10.1111/1755-0998.12689 <https://doi.org/10.1111/1755-0998.12689> , hdl:10013/epic.51073 EPIC3Molecular Ecology Resources, WILEY-BLACKWELL PUBLISHING, ISSN: 1755-098X Article isiRev 2017 ftawi https://doi.org/10.1111/1755-0998.12689 2024-06-24T04:17:43Z Reliable information on past and present vegetation is important to project future changes, especially for rapidly transitioning areas such as the boreal treeline. To study past vegetation, pollen analysis is common, while current vegetation is usually assessed by field surveys. Application of detailed sedimentary DNA (sedDNA) records has the potential to enhance our understanding of vegetation changes, but studies systematically investigating the power of this proxy are rare to date. This study compares sedDNA metabarcoding and pollen records from surface sediments of 31 lakes along a north-south gradient of increasing forest cover in northern Siberia (Taymyr peninsula) with data from field surveys in the surroundings of the lakes. SedDNA metabarcoding recorded 114 plant taxa, about half of them to species level, while pollen analyses identified 43 taxa; both exceeding the 31 taxa found by vegetation field surveys. Increasing Larix percentages from north to south were consistently recorded by all three methods and principal component analyses based on percentage data of vegetation surveys and DNA sequences separated tundra from forested sites. Comparisons of the ordinations using Procrustes and PROTEST analyses show a significant fit among all compared pairs of records. Despite similarities of sedDNA and pollen records, certain idiosyncrasies, such as high percentages of Alnus and Betula in all pollen and high percentages of Salix in all sedDNA spectra are observable. Our results from the tundra to single-tree tundra transition zone show that sedDNA analyses perform better than pollen in recording site-specific richness (i.e. presence/absence of taxa in the vicinity of the lake) and perform as well as pollen in tracing vegetation composition. Article in Journal/Newspaper Taymyr Taymyr Peninsula Tundra Siberia Alfred Wegener Institute for Polar- and Marine Research (AWI): ePIC (electronic Publication Information Center) Molecular Ecology Resources 17 6 e46 e62 |
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Alfred Wegener Institute for Polar- and Marine Research (AWI): ePIC (electronic Publication Information Center) |
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ftawi |
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unknown |
description |
Reliable information on past and present vegetation is important to project future changes, especially for rapidly transitioning areas such as the boreal treeline. To study past vegetation, pollen analysis is common, while current vegetation is usually assessed by field surveys. Application of detailed sedimentary DNA (sedDNA) records has the potential to enhance our understanding of vegetation changes, but studies systematically investigating the power of this proxy are rare to date. This study compares sedDNA metabarcoding and pollen records from surface sediments of 31 lakes along a north-south gradient of increasing forest cover in northern Siberia (Taymyr peninsula) with data from field surveys in the surroundings of the lakes. SedDNA metabarcoding recorded 114 plant taxa, about half of them to species level, while pollen analyses identified 43 taxa; both exceeding the 31 taxa found by vegetation field surveys. Increasing Larix percentages from north to south were consistently recorded by all three methods and principal component analyses based on percentage data of vegetation surveys and DNA sequences separated tundra from forested sites. Comparisons of the ordinations using Procrustes and PROTEST analyses show a significant fit among all compared pairs of records. Despite similarities of sedDNA and pollen records, certain idiosyncrasies, such as high percentages of Alnus and Betula in all pollen and high percentages of Salix in all sedDNA spectra are observable. Our results from the tundra to single-tree tundra transition zone show that sedDNA analyses perform better than pollen in recording site-specific richness (i.e. presence/absence of taxa in the vicinity of the lake) and perform as well as pollen in tracing vegetation composition. |
format |
Article in Journal/Newspaper |
author |
Niemeyer, Bastian Epp, Laura Stoof-Leichsenring, Kathleen Pestryakova, L. A. Herzschuh, Ulrike |
spellingShingle |
Niemeyer, Bastian Epp, Laura Stoof-Leichsenring, Kathleen Pestryakova, L. A. Herzschuh, Ulrike A comparison of sedimentary DNA and pollen from lake sediments in recording vegetation composition at the Siberian treeline |
author_facet |
Niemeyer, Bastian Epp, Laura Stoof-Leichsenring, Kathleen Pestryakova, L. A. Herzschuh, Ulrike |
author_sort |
Niemeyer, Bastian |
title |
A comparison of sedimentary DNA and pollen from lake sediments in recording vegetation composition at the Siberian treeline |
title_short |
A comparison of sedimentary DNA and pollen from lake sediments in recording vegetation composition at the Siberian treeline |
title_full |
A comparison of sedimentary DNA and pollen from lake sediments in recording vegetation composition at the Siberian treeline |
title_fullStr |
A comparison of sedimentary DNA and pollen from lake sediments in recording vegetation composition at the Siberian treeline |
title_full_unstemmed |
A comparison of sedimentary DNA and pollen from lake sediments in recording vegetation composition at the Siberian treeline |
title_sort |
comparison of sedimentary dna and pollen from lake sediments in recording vegetation composition at the siberian treeline |
publisher |
WILEY-BLACKWELL PUBLISHING |
publishDate |
2017 |
url |
https://epic.awi.de/id/eprint/44830/ https://hdl.handle.net/10013/epic.51073 |
genre |
Taymyr Taymyr Peninsula Tundra Siberia |
genre_facet |
Taymyr Taymyr Peninsula Tundra Siberia |
op_source |
EPIC3Molecular Ecology Resources, WILEY-BLACKWELL PUBLISHING, ISSN: 1755-098X |
op_relation |
Niemeyer, B. , Epp, L. , Stoof-Leichsenring, K. orcid:0000-0002-6609-3217 , Pestryakova, L. A. and Herzschuh, U. orcid:0000-0003-0999-1261 (2017) A comparison of sedimentary DNA and pollen from lake sediments in recording vegetation composition at the Siberian treeline , Molecular Ecology Resources . doi:10.1111/1755-0998.12689 <https://doi.org/10.1111/1755-0998.12689> , hdl:10013/epic.51073 |
op_doi |
https://doi.org/10.1111/1755-0998.12689 |
container_title |
Molecular Ecology Resources |
container_volume |
17 |
container_issue |
6 |
container_start_page |
e46 |
op_container_end_page |
e62 |
_version_ |
1810483227184332800 |