Characterization of facultative oligotrophic bacteria from polar seas by analysis of their fatty acids and 16S rDNA sequences
One hundred and seventy three bacterial strains, isolated previously after enrichment under oligotrophic, psychrophilic conditions from Arctic (98 strains) and Antarctic seawater (75 strains), were characterized by gas-liquid chromatographic analysis of their fatty acid compositions. By numerical an...
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ftawi:oai:epic.awi.de:4403 2023-09-05T13:14:56+02:00 Characterization of facultative oligotrophic bacteria from polar seas by analysis of their fatty acids and 16S rDNA sequences Mergaert, J. Verhelst, A. Cnockaert, M. A. Tan, T.-L. Swings, J. 2001 https://epic.awi.de/id/eprint/4403/ https://hdl.handle.net/10013/epic.14978 unknown Mergaert, J. , Verhelst, A. , Cnockaert, M. A. , Tan, T. L. and Swings, J. (2001) Characterization of facultative oligotrophic bacteria from polar seas by analysis of their fatty acids and 16S rDNA sequences , Systematic and Applied Microbiology, 24 , 98 - 107 . hdl:10013/epic.14978 EPIC3Systematic and Applied Microbiology, 24, pp. 98 - 107, ISBN: 0723-2020012401-098 Article isiRev 2001 ftawi 2023-08-22T19:44:40Z One hundred and seventy three bacterial strains, isolated previously after enrichment under oligotrophic, psychrophilic conditions from Arctic (98 strains) and Antarctic seawater (75 strains), were characterized by gas-liquid chromatographic analysis of their fatty acid compositions. By numerical analysis, 8 clusters, containing 2 to 59 strains, could be delineated, and 8 strains formed separate branches. Five clusters contained strains from both poles, two minor clusters were confined to Arctic isolates, and one cluster consisted of Antarctic isolates only. The 16S rRNA genes from 23 strains, representing the different fatty acid profile clusters and including the unclustered strains, were sequenced. The sequences grouped with the alpha and gamma Proteobacteria, the high percent G+C Gram-positives, and the Cytophaga-Flavobacterium-Bacteroides branch. The sequences of strains from 4 clusters and of 7 unclustered strains were closely related (sequence similarities above 97%) to reference sequences of Sulfitobacter mediterraneus, Halomonas variabilis, Alteromonas macleodii, Pseudoalteromonas species, Shewanella frigidimarina, and Rhodococcus fascians. Strains from the other four clusters and an unclustered strain showed sequence similarities below 97% with nearest named neighbours, including Rhizobium, Glaciecola, Pseudomonas, Alteromonas macleodii and Cytophaga marinoflava, indicating that the clusters which they represent form as yet unnamed taxa. Article in Journal/Newspaper Antarc* Antarctic Arctic Alfred Wegener Institute for Polar- and Marine Research (AWI): ePIC (electronic Publication Information Center) Antarctic Arctic |
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Open Polar |
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Alfred Wegener Institute for Polar- and Marine Research (AWI): ePIC (electronic Publication Information Center) |
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ftawi |
language |
unknown |
description |
One hundred and seventy three bacterial strains, isolated previously after enrichment under oligotrophic, psychrophilic conditions from Arctic (98 strains) and Antarctic seawater (75 strains), were characterized by gas-liquid chromatographic analysis of their fatty acid compositions. By numerical analysis, 8 clusters, containing 2 to 59 strains, could be delineated, and 8 strains formed separate branches. Five clusters contained strains from both poles, two minor clusters were confined to Arctic isolates, and one cluster consisted of Antarctic isolates only. The 16S rRNA genes from 23 strains, representing the different fatty acid profile clusters and including the unclustered strains, were sequenced. The sequences grouped with the alpha and gamma Proteobacteria, the high percent G+C Gram-positives, and the Cytophaga-Flavobacterium-Bacteroides branch. The sequences of strains from 4 clusters and of 7 unclustered strains were closely related (sequence similarities above 97%) to reference sequences of Sulfitobacter mediterraneus, Halomonas variabilis, Alteromonas macleodii, Pseudoalteromonas species, Shewanella frigidimarina, and Rhodococcus fascians. Strains from the other four clusters and an unclustered strain showed sequence similarities below 97% with nearest named neighbours, including Rhizobium, Glaciecola, Pseudomonas, Alteromonas macleodii and Cytophaga marinoflava, indicating that the clusters which they represent form as yet unnamed taxa. |
format |
Article in Journal/Newspaper |
author |
Mergaert, J. Verhelst, A. Cnockaert, M. A. Tan, T.-L. Swings, J. |
spellingShingle |
Mergaert, J. Verhelst, A. Cnockaert, M. A. Tan, T.-L. Swings, J. Characterization of facultative oligotrophic bacteria from polar seas by analysis of their fatty acids and 16S rDNA sequences |
author_facet |
Mergaert, J. Verhelst, A. Cnockaert, M. A. Tan, T.-L. Swings, J. |
author_sort |
Mergaert, J. |
title |
Characterization of facultative oligotrophic bacteria from polar seas by analysis of their fatty acids and 16S rDNA sequences |
title_short |
Characterization of facultative oligotrophic bacteria from polar seas by analysis of their fatty acids and 16S rDNA sequences |
title_full |
Characterization of facultative oligotrophic bacteria from polar seas by analysis of their fatty acids and 16S rDNA sequences |
title_fullStr |
Characterization of facultative oligotrophic bacteria from polar seas by analysis of their fatty acids and 16S rDNA sequences |
title_full_unstemmed |
Characterization of facultative oligotrophic bacteria from polar seas by analysis of their fatty acids and 16S rDNA sequences |
title_sort |
characterization of facultative oligotrophic bacteria from polar seas by analysis of their fatty acids and 16s rdna sequences |
publishDate |
2001 |
url |
https://epic.awi.de/id/eprint/4403/ https://hdl.handle.net/10013/epic.14978 |
geographic |
Antarctic Arctic |
geographic_facet |
Antarctic Arctic |
genre |
Antarc* Antarctic Arctic |
genre_facet |
Antarc* Antarctic Arctic |
op_source |
EPIC3Systematic and Applied Microbiology, 24, pp. 98 - 107, ISBN: 0723-2020012401-098 |
op_relation |
Mergaert, J. , Verhelst, A. , Cnockaert, M. A. , Tan, T. L. and Swings, J. (2001) Characterization of facultative oligotrophic bacteria from polar seas by analysis of their fatty acids and 16S rDNA sequences , Systematic and Applied Microbiology, 24 , 98 - 107 . hdl:10013/epic.14978 |
_version_ |
1776196803401613312 |