Biodiversity assessment and transcriptomic analyzes of eukaryotic sea ice diatoms of the central Arctic Ocean
Sea ice is a large environment controlled by a number of abiotic factors (i.e. low temperatures and high salinities). Despite these harsh conditions, it is inhabited by a diverse community and significantly contributing to primary production in ice-covered regions. While this biodiversity has been i...
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ftawi:oai:epic.awi.de:43869 2023-05-15T14:25:53+02:00 Biodiversity assessment and transcriptomic analyzes of eukaryotic sea ice diatoms of the central Arctic Ocean Stecher, Anique Franze, Madlen Groth, Marco Harms, Lars Glöckner, Gernot Heinrich, Sandra Kroth, Peter G. Valentin, Klaus-Ulrich 2015-08 https://epic.awi.de/id/eprint/43869/ https://hdl.handle.net/10013/epic.50451 unknown Stecher, A. , Franze, M. , Groth, M. , Harms, L. orcid:0000-0001-7620-0613 , Glöckner, G. , Heinrich, S. , Kroth, P. G. and Valentin, K. U. (2015) Biodiversity assessment and transcriptomic analyzes of eukaryotic sea ice diatoms of the central Arctic Ocean , 6th European Phycological Congress . hdl:10013/epic.50451 EPIC36th European Phycological Congress Conference notRev 2015 ftawi 2021-12-24T15:42:41Z Sea ice is a large environment controlled by a number of abiotic factors (i.e. low temperatures and high salinities). Despite these harsh conditions, it is inhabited by a diverse community and significantly contributing to primary production in ice-covered regions. While this biodiversity has been investigated in the past by classical methods, little is known about its functional biodiversity, i.e. which species are actively contributing with which functions to the community. By sequencing 18S rRNA and rDNA amplicons, we were able to compare the “total” biodiversity (rDNA-based) with the “active” biodiversity (rRNA-based) and found an over-representation of certain groups (i.e. Bacillariophyceae and Ciliophora) in the active part of the community. Furthermore, we were able to isolate an abundant naviculoid sea ice diatom member (CCMP2297) of the Arctic sea ice community and conducted temperature stress experiments (10 °C, 5 °C, -2 °C, -5 °C) and analyzed not only physiological responses to high and cold temperature stress, but also the molecular responses to thermal stress by sequencing the transcriptome. We found that based on physiological parameters this diatom has a broad thermal range (5 °C to -5 °C) but significantly changes its gene expression pattern at higher temperatures. Conference Object Arctic Arctic Arctic Ocean Sea ice Alfred Wegener Institute for Polar- and Marine Research (AWI): ePIC (electronic Publication Information Center) Arctic Arctic Ocean |
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Alfred Wegener Institute for Polar- and Marine Research (AWI): ePIC (electronic Publication Information Center) |
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Sea ice is a large environment controlled by a number of abiotic factors (i.e. low temperatures and high salinities). Despite these harsh conditions, it is inhabited by a diverse community and significantly contributing to primary production in ice-covered regions. While this biodiversity has been investigated in the past by classical methods, little is known about its functional biodiversity, i.e. which species are actively contributing with which functions to the community. By sequencing 18S rRNA and rDNA amplicons, we were able to compare the “total” biodiversity (rDNA-based) with the “active” biodiversity (rRNA-based) and found an over-representation of certain groups (i.e. Bacillariophyceae and Ciliophora) in the active part of the community. Furthermore, we were able to isolate an abundant naviculoid sea ice diatom member (CCMP2297) of the Arctic sea ice community and conducted temperature stress experiments (10 °C, 5 °C, -2 °C, -5 °C) and analyzed not only physiological responses to high and cold temperature stress, but also the molecular responses to thermal stress by sequencing the transcriptome. We found that based on physiological parameters this diatom has a broad thermal range (5 °C to -5 °C) but significantly changes its gene expression pattern at higher temperatures. |
format |
Conference Object |
author |
Stecher, Anique Franze, Madlen Groth, Marco Harms, Lars Glöckner, Gernot Heinrich, Sandra Kroth, Peter G. Valentin, Klaus-Ulrich |
spellingShingle |
Stecher, Anique Franze, Madlen Groth, Marco Harms, Lars Glöckner, Gernot Heinrich, Sandra Kroth, Peter G. Valentin, Klaus-Ulrich Biodiversity assessment and transcriptomic analyzes of eukaryotic sea ice diatoms of the central Arctic Ocean |
author_facet |
Stecher, Anique Franze, Madlen Groth, Marco Harms, Lars Glöckner, Gernot Heinrich, Sandra Kroth, Peter G. Valentin, Klaus-Ulrich |
author_sort |
Stecher, Anique |
title |
Biodiversity assessment and transcriptomic analyzes of eukaryotic sea ice diatoms of the central Arctic Ocean |
title_short |
Biodiversity assessment and transcriptomic analyzes of eukaryotic sea ice diatoms of the central Arctic Ocean |
title_full |
Biodiversity assessment and transcriptomic analyzes of eukaryotic sea ice diatoms of the central Arctic Ocean |
title_fullStr |
Biodiversity assessment and transcriptomic analyzes of eukaryotic sea ice diatoms of the central Arctic Ocean |
title_full_unstemmed |
Biodiversity assessment and transcriptomic analyzes of eukaryotic sea ice diatoms of the central Arctic Ocean |
title_sort |
biodiversity assessment and transcriptomic analyzes of eukaryotic sea ice diatoms of the central arctic ocean |
publishDate |
2015 |
url |
https://epic.awi.de/id/eprint/43869/ https://hdl.handle.net/10013/epic.50451 |
geographic |
Arctic Arctic Ocean |
geographic_facet |
Arctic Arctic Ocean |
genre |
Arctic Arctic Arctic Ocean Sea ice |
genre_facet |
Arctic Arctic Arctic Ocean Sea ice |
op_source |
EPIC36th European Phycological Congress |
op_relation |
Stecher, A. , Franze, M. , Groth, M. , Harms, L. orcid:0000-0001-7620-0613 , Glöckner, G. , Heinrich, S. , Kroth, P. G. and Valentin, K. U. (2015) Biodiversity assessment and transcriptomic analyzes of eukaryotic sea ice diatoms of the central Arctic Ocean , 6th European Phycological Congress . hdl:10013/epic.50451 |
_version_ |
1766298365390225408 |