Temperature Modulates the Effects of Ocean Acidification on Intestinal Ion Transport in Atlantic Cod, Gadus morhua
CO2-driven seawater acidification has been demonstrated to enhance intestinal bicarbonate secretion rates in teleosts, leading to an increased release of CaCO3 under simulated ocean acidification scenarios. In this study, we investigated if increasing CO2 levels stimulate the intestinal acid–base re...
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ftawi:oai:epic.awi.de:43035 2024-09-15T17:55:27+00:00 Temperature Modulates the Effects of Ocean Acidification on Intestinal Ion Transport in Atlantic Cod, Gadus morhua Hu, Marian Y.A. Michael, Katharina Kreiß, Cornelia Stumpp, Meike Dupont, Sam Tseng, Y.-C. Lucassen, Magnus 2016-06-02 application/pdf https://epic.awi.de/id/eprint/43035/ https://epic.awi.de/id/eprint/43035/1/Hu_etal2016.pdf https://hdl.handle.net/10013/epic.49556 https://hdl.handle.net/10013/epic.49556.d001 unknown https://epic.awi.de/id/eprint/43035/1/Hu_etal2016.pdf https://hdl.handle.net/10013/epic.49556.d001 Hu, M. Y. , Michael, K. , Kreiß, C. , Stumpp, M. , Dupont, S. , Tseng, Y. C. and Lucassen, M. orcid:0000-0003-4276-4781 (2016) Temperature Modulates the Effects of Ocean Acidification on Intestinal Ion Transport in Atlantic Cod, Gadus morhua , Front. Physiol., 7 (198) . doi:10.3389/fphys.2016.00198 <https://doi.org/10.3389/fphys.2016.00198> , hdl:10013/epic.49556 EPIC3Front. Physiol., 7(198), ISSN: 1664-042X Article isiRev 2016 ftawi https://doi.org/10.3389/fphys.2016.00198 2024-06-24T04:16:35Z CO2-driven seawater acidification has been demonstrated to enhance intestinal bicarbonate secretion rates in teleosts, leading to an increased release of CaCO3 under simulated ocean acidification scenarios. In this study, we investigated if increasing CO2 levels stimulate the intestinal acid–base regulatory machinery of Atlantic cod (Gadus morhua) and whether temperatures at the upper limit of thermal tolerance stimulate or counteract ion regulatory capacities. Juvenile G. morhua were acclimated for 4 weeks to three CO2 levels (550, 1200, and 2200 μatm) covering present and near-future natural variability, at optimum (10°C) and summer maximum temperature (18°C), respectively. Immunohistochemical analyses revealed the subcellular localization of ion transporters, including Na+/K+-ATPase (NKA), Na+/H+-exchanger 3 (NHE3), Na+/HCO−3 cotransporter (NBC1), pendrin-like Cl−/HCO−3 exchanger (SLC26a6), V-type H+-ATPase subunit a (VHA), and Cl− channel 3 (CLC3) in epithelial cells of the anterior intestine. At 10°C, proteins and mRNA were generally up-regulated for most transporters in the intestinal epithelium after acclimation to higher CO2 levels. This supports recent findings demonstrating increased intestinal HCO−3 secretion rates in response to CO2 induced seawater acidification. At 18°C, mRNA expression and protein concentrations of most ion transporters remained unchanged or were even decreased, suggesting thermal compensation. This response may be energetically favorable to retain blood HCO−3 levels to stabilize pHe, but may negatively affect intestinal salt and water resorption of marine teleosts in future oceans. Article in Journal/Newspaper atlantic cod Gadus morhua Ocean acidification Alfred Wegener Institute for Polar- and Marine Research (AWI): ePIC (electronic Publication Information Center) Frontiers in Physiology 7 |
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Alfred Wegener Institute for Polar- and Marine Research (AWI): ePIC (electronic Publication Information Center) |
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ftawi |
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description |
CO2-driven seawater acidification has been demonstrated to enhance intestinal bicarbonate secretion rates in teleosts, leading to an increased release of CaCO3 under simulated ocean acidification scenarios. In this study, we investigated if increasing CO2 levels stimulate the intestinal acid–base regulatory machinery of Atlantic cod (Gadus morhua) and whether temperatures at the upper limit of thermal tolerance stimulate or counteract ion regulatory capacities. Juvenile G. morhua were acclimated for 4 weeks to three CO2 levels (550, 1200, and 2200 μatm) covering present and near-future natural variability, at optimum (10°C) and summer maximum temperature (18°C), respectively. Immunohistochemical analyses revealed the subcellular localization of ion transporters, including Na+/K+-ATPase (NKA), Na+/H+-exchanger 3 (NHE3), Na+/HCO−3 cotransporter (NBC1), pendrin-like Cl−/HCO−3 exchanger (SLC26a6), V-type H+-ATPase subunit a (VHA), and Cl− channel 3 (CLC3) in epithelial cells of the anterior intestine. At 10°C, proteins and mRNA were generally up-regulated for most transporters in the intestinal epithelium after acclimation to higher CO2 levels. This supports recent findings demonstrating increased intestinal HCO−3 secretion rates in response to CO2 induced seawater acidification. At 18°C, mRNA expression and protein concentrations of most ion transporters remained unchanged or were even decreased, suggesting thermal compensation. This response may be energetically favorable to retain blood HCO−3 levels to stabilize pHe, but may negatively affect intestinal salt and water resorption of marine teleosts in future oceans. |
format |
Article in Journal/Newspaper |
author |
Hu, Marian Y.A. Michael, Katharina Kreiß, Cornelia Stumpp, Meike Dupont, Sam Tseng, Y.-C. Lucassen, Magnus |
spellingShingle |
Hu, Marian Y.A. Michael, Katharina Kreiß, Cornelia Stumpp, Meike Dupont, Sam Tseng, Y.-C. Lucassen, Magnus Temperature Modulates the Effects of Ocean Acidification on Intestinal Ion Transport in Atlantic Cod, Gadus morhua |
author_facet |
Hu, Marian Y.A. Michael, Katharina Kreiß, Cornelia Stumpp, Meike Dupont, Sam Tseng, Y.-C. Lucassen, Magnus |
author_sort |
Hu, Marian Y.A. |
title |
Temperature Modulates the Effects of Ocean Acidification on Intestinal Ion Transport in Atlantic Cod, Gadus morhua |
title_short |
Temperature Modulates the Effects of Ocean Acidification on Intestinal Ion Transport in Atlantic Cod, Gadus morhua |
title_full |
Temperature Modulates the Effects of Ocean Acidification on Intestinal Ion Transport in Atlantic Cod, Gadus morhua |
title_fullStr |
Temperature Modulates the Effects of Ocean Acidification on Intestinal Ion Transport in Atlantic Cod, Gadus morhua |
title_full_unstemmed |
Temperature Modulates the Effects of Ocean Acidification on Intestinal Ion Transport in Atlantic Cod, Gadus morhua |
title_sort |
temperature modulates the effects of ocean acidification on intestinal ion transport in atlantic cod, gadus morhua |
publishDate |
2016 |
url |
https://epic.awi.de/id/eprint/43035/ https://epic.awi.de/id/eprint/43035/1/Hu_etal2016.pdf https://hdl.handle.net/10013/epic.49556 https://hdl.handle.net/10013/epic.49556.d001 |
genre |
atlantic cod Gadus morhua Ocean acidification |
genre_facet |
atlantic cod Gadus morhua Ocean acidification |
op_source |
EPIC3Front. Physiol., 7(198), ISSN: 1664-042X |
op_relation |
https://epic.awi.de/id/eprint/43035/1/Hu_etal2016.pdf https://hdl.handle.net/10013/epic.49556.d001 Hu, M. Y. , Michael, K. , Kreiß, C. , Stumpp, M. , Dupont, S. , Tseng, Y. C. and Lucassen, M. orcid:0000-0003-4276-4781 (2016) Temperature Modulates the Effects of Ocean Acidification on Intestinal Ion Transport in Atlantic Cod, Gadus morhua , Front. Physiol., 7 (198) . doi:10.3389/fphys.2016.00198 <https://doi.org/10.3389/fphys.2016.00198> , hdl:10013/epic.49556 |
op_doi |
https://doi.org/10.3389/fphys.2016.00198 |
container_title |
Frontiers in Physiology |
container_volume |
7 |
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1810431736563105792 |