Adjustments of molecular key components of branchial ion and pH regulation in Atlantic cod (Gadus morhua) in response to ocean acidification and warming.
Marine teleost fish sustain compensation of extracellular pH after exposure to hypercapnia by means of efficient ion and acid-base regulation. Elevated rates of ion and acid-base regulation under hypercapnia may be stimulated further by elevated temperature. Here, we characterized the regulation of...
Published in: | Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology |
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Online Access: | https://epic.awi.de/id/eprint/40410/ https://epic.awi.de/id/eprint/40410/1/Michael_etal2016.pdf https://hdl.handle.net/10013/epic.47473 https://hdl.handle.net/10013/epic.47473.d001 |
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ftawi:oai:epic.awi.de:40410 2024-09-15T17:55:28+00:00 Adjustments of molecular key components of branchial ion and pH regulation in Atlantic cod (Gadus morhua) in response to ocean acidification and warming. Michael, Katharina Kreiß, Cornelia Hu, Marian Y.A. Koschnick, Nils Bickmeyer, Ulf Dupont, S. Pörtner, Hans-Otto Lucassen, Magnus 2016-03 application/pdf https://epic.awi.de/id/eprint/40410/ https://epic.awi.de/id/eprint/40410/1/Michael_etal2016.pdf https://hdl.handle.net/10013/epic.47473 https://hdl.handle.net/10013/epic.47473.d001 unknown ELSEVIER SCIENCE INC https://epic.awi.de/id/eprint/40410/1/Michael_etal2016.pdf https://hdl.handle.net/10013/epic.47473.d001 Michael, K. , Kreiß, C. , Hu, M. Y. , Koschnick, N. , Bickmeyer, U. orcid:0000-0002-5351-2902 , Dupont, S. , Pörtner, H. O. orcid:0000-0001-6535-6575 and Lucassen, M. orcid:0000-0003-4276-4781 (2016) Adjustments of molecular key components of branchial ion and pH regulation in Atlantic cod (Gadus morhua) in response to ocean acidification and warming. , Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology, 193 , pp. 33-46 . doi:10.1016/j.cbpb.2015.12.006 <https://doi.org/10.1016/j.cbpb.2015.12.006> , hdl:10013/epic.47473 EPIC3Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology, ELSEVIER SCIENCE INC, 193, pp. 33-46, ISSN: 1096-4959 Article isiRev 2016 ftawi 2024-06-24T04:14:20Z Marine teleost fish sustain compensation of extracellular pH after exposure to hypercapnia by means of efficient ion and acid-base regulation. Elevated rates of ion and acid-base regulation under hypercapnia may be stimulated further by elevated temperature. Here, we characterized the regulation of transepithelial ion transporters (NKCC1, NBC1, SLC26A6, NHE1 and 2) and ATPases (Na(+)/K(+) ATPase and V-type H(+) ATPase) in gills of Atlantic cod (Gadus morhua) after 4weeks of exposure to ambient and future PCO2 levels (550μatm, 1200μatm, 2200μatm) at optimum (10°C) and summer maximum temperature (18°C), respectively. Gene expression of most branchial ion transporters revealed temperature- and dose-dependent responses to elevated PCO2. Transcriptional regulation resulted in stable protein expression at 10°C, whereas expression of most transport proteins increased at medium PCO2 and 18°C. mRNA and protein expression of distinct ion transport proteins were closely co-regulated, substantiating cellular functional relationships. Na(+)/K(+) ATPase capacities were PCO2 independent, but increased with acclimation temperature, whereas H(+) ATPase capacities were thermally compensated but decreased at medium PCO2 and 10°C. When functional capacities of branchial ATPases were compared with mitochondrial F1Fo ATP-synthase strong correlations of F1Fo ATP-synthase and ATPase capacities generally indicate close coordination of branchial aerobic ATP demand and supply. Our data indicate physiological plasticity in the gills of cod to adjust to a warming, acidifying ocean within limits. In light of the interacting and non-linear, dose-dependent effects of both climate factors the role of these mechanisms in shaping resilience under climate change remains to be explored Article in Journal/Newspaper atlantic cod Gadus morhua Ocean acidification Alfred Wegener Institute for Polar- and Marine Research (AWI): ePIC (electronic Publication Information Center) Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology 193 33 46 |
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Open Polar |
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Alfred Wegener Institute for Polar- and Marine Research (AWI): ePIC (electronic Publication Information Center) |
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ftawi |
language |
unknown |
description |
Marine teleost fish sustain compensation of extracellular pH after exposure to hypercapnia by means of efficient ion and acid-base regulation. Elevated rates of ion and acid-base regulation under hypercapnia may be stimulated further by elevated temperature. Here, we characterized the regulation of transepithelial ion transporters (NKCC1, NBC1, SLC26A6, NHE1 and 2) and ATPases (Na(+)/K(+) ATPase and V-type H(+) ATPase) in gills of Atlantic cod (Gadus morhua) after 4weeks of exposure to ambient and future PCO2 levels (550μatm, 1200μatm, 2200μatm) at optimum (10°C) and summer maximum temperature (18°C), respectively. Gene expression of most branchial ion transporters revealed temperature- and dose-dependent responses to elevated PCO2. Transcriptional regulation resulted in stable protein expression at 10°C, whereas expression of most transport proteins increased at medium PCO2 and 18°C. mRNA and protein expression of distinct ion transport proteins were closely co-regulated, substantiating cellular functional relationships. Na(+)/K(+) ATPase capacities were PCO2 independent, but increased with acclimation temperature, whereas H(+) ATPase capacities were thermally compensated but decreased at medium PCO2 and 10°C. When functional capacities of branchial ATPases were compared with mitochondrial F1Fo ATP-synthase strong correlations of F1Fo ATP-synthase and ATPase capacities generally indicate close coordination of branchial aerobic ATP demand and supply. Our data indicate physiological plasticity in the gills of cod to adjust to a warming, acidifying ocean within limits. In light of the interacting and non-linear, dose-dependent effects of both climate factors the role of these mechanisms in shaping resilience under climate change remains to be explored |
format |
Article in Journal/Newspaper |
author |
Michael, Katharina Kreiß, Cornelia Hu, Marian Y.A. Koschnick, Nils Bickmeyer, Ulf Dupont, S. Pörtner, Hans-Otto Lucassen, Magnus |
spellingShingle |
Michael, Katharina Kreiß, Cornelia Hu, Marian Y.A. Koschnick, Nils Bickmeyer, Ulf Dupont, S. Pörtner, Hans-Otto Lucassen, Magnus Adjustments of molecular key components of branchial ion and pH regulation in Atlantic cod (Gadus morhua) in response to ocean acidification and warming. |
author_facet |
Michael, Katharina Kreiß, Cornelia Hu, Marian Y.A. Koschnick, Nils Bickmeyer, Ulf Dupont, S. Pörtner, Hans-Otto Lucassen, Magnus |
author_sort |
Michael, Katharina |
title |
Adjustments of molecular key components of branchial ion and pH regulation in Atlantic cod (Gadus morhua) in response to ocean acidification and warming. |
title_short |
Adjustments of molecular key components of branchial ion and pH regulation in Atlantic cod (Gadus morhua) in response to ocean acidification and warming. |
title_full |
Adjustments of molecular key components of branchial ion and pH regulation in Atlantic cod (Gadus morhua) in response to ocean acidification and warming. |
title_fullStr |
Adjustments of molecular key components of branchial ion and pH regulation in Atlantic cod (Gadus morhua) in response to ocean acidification and warming. |
title_full_unstemmed |
Adjustments of molecular key components of branchial ion and pH regulation in Atlantic cod (Gadus morhua) in response to ocean acidification and warming. |
title_sort |
adjustments of molecular key components of branchial ion and ph regulation in atlantic cod (gadus morhua) in response to ocean acidification and warming. |
publisher |
ELSEVIER SCIENCE INC |
publishDate |
2016 |
url |
https://epic.awi.de/id/eprint/40410/ https://epic.awi.de/id/eprint/40410/1/Michael_etal2016.pdf https://hdl.handle.net/10013/epic.47473 https://hdl.handle.net/10013/epic.47473.d001 |
genre |
atlantic cod Gadus morhua Ocean acidification |
genre_facet |
atlantic cod Gadus morhua Ocean acidification |
op_source |
EPIC3Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology, ELSEVIER SCIENCE INC, 193, pp. 33-46, ISSN: 1096-4959 |
op_relation |
https://epic.awi.de/id/eprint/40410/1/Michael_etal2016.pdf https://hdl.handle.net/10013/epic.47473.d001 Michael, K. , Kreiß, C. , Hu, M. Y. , Koschnick, N. , Bickmeyer, U. orcid:0000-0002-5351-2902 , Dupont, S. , Pörtner, H. O. orcid:0000-0001-6535-6575 and Lucassen, M. orcid:0000-0003-4276-4781 (2016) Adjustments of molecular key components of branchial ion and pH regulation in Atlantic cod (Gadus morhua) in response to ocean acidification and warming. , Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology, 193 , pp. 33-46 . doi:10.1016/j.cbpb.2015.12.006 <https://doi.org/10.1016/j.cbpb.2015.12.006> , hdl:10013/epic.47473 |
container_title |
Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology |
container_volume |
193 |
container_start_page |
33 |
op_container_end_page |
46 |
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1810431749300158464 |