Growth and bioactive secondary metabolites of arctic Protoceratium reticulatum (Dinophyceae)
Harmful algal blooms are mainly caused by marine dinoflagellates and are known to produce potent toxins that may affect the ecosystem, human activities and health. Such events have increased in frequency and intensity worldwide in the past decades. Numerous processes involved in Global Change are am...
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2016
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Online Access: | https://epic.awi.de/id/eprint/40227/ https://epic.awi.de/id/eprint/40227/1/2016_Sala_Perez_HA.pdf https://hdl.handle.net/10013/epic.47331 https://hdl.handle.net/10013/epic.47331.d001 |
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ftawi:oai:epic.awi.de:40227 2023-05-15T14:27:15+02:00 Growth and bioactive secondary metabolites of arctic Protoceratium reticulatum (Dinophyceae) Sala-Pérez, Manuel Alpermann, Tilman Krock, Bernd Tillmann, Urban 2016 application/pdf https://epic.awi.de/id/eprint/40227/ https://epic.awi.de/id/eprint/40227/1/2016_Sala_Perez_HA.pdf https://hdl.handle.net/10013/epic.47331 https://hdl.handle.net/10013/epic.47331.d001 unknown ELSEVIER SCIENCE BV https://epic.awi.de/id/eprint/40227/1/2016_Sala_Perez_HA.pdf https://hdl.handle.net/10013/epic.47331.d001 Sala-Pérez, M. , Alpermann, T. , Krock, B. orcid:0000-0003-4022-9101 and Tillmann, U. orcid:0000-0002-8207-4382 (2016) Growth and bioactive secondary metabolites of arctic Protoceratium reticulatum (Dinophyceae) , Harmful Algae, 55 , pp. 85-96 . doi:10.1016/j.hal.2016.02.004 <https://doi.org/10.1016/j.hal.2016.02.004> , hdl:10013/epic.47331 EPIC3Harmful Algae, ELSEVIER SCIENCE BV, 55, pp. 85-96, ISSN: 1568-9883 Article isiRev 2016 ftawi https://doi.org/10.1016/j.hal.2016.02.004 2021-12-24T15:41:21Z Harmful algal blooms are mainly caused by marine dinoflagellates and are known to produce potent toxins that may affect the ecosystem, human activities and health. Such events have increased in frequency and intensity worldwide in the past decades. Numerous processes involved in Global Change are amplified in the Arctic, but little is known about species specific responses of arctic dinoflagellates. The aim of this work was to perform an exhaustive morphological, phylogenetical and toxinological characterization of Greenland Protoceratium reticulatum and, in addition, to test the effect of temperature on growth and production of bioactive secondary metabolites. Seven clonal isolates, the first isolates of P. reticulatum available from arctic waters, were phylogenetically characterized by analysis of the LSU rDNA. Six isolates were further characterized morphologically and were shown to produce both yessotoxins (YTX) and lytic compounds, representing the first report of allelochemical activity in P. reticulatum. As shown for one of the isolates, growth was strongly affected by temperature with a maximum growth rate at 15 8C, a significant but slow growth at 1 8C, and cell death at 25 8C, suggesting an adaptation of P. reticulatum to temperate waters. Temperature had no major effect on total YTX cell quota or lytic activity but both were affected by the growth phase with a significant increase at stationary phase. A comparison of six isolates at a fixed temperature of 10 8C showed high intraspecific variability for all three physiological parameters tested. Growth rate varied from 0.06 to 0.19 d�1, and total YTX concentration ranged from 0.3 to 15.0 pg YTX cell�1 and from 0.5 to 31.0 pg YTX cell�1 at exponential and stationary phase, respectively. All six isolates performed lytic activity; however, for two isolates lytic activity was only detectable at higher cell densities in stationary phase. Article in Journal/Newspaper Arctic Arctic Greenland Alfred Wegener Institute for Polar- and Marine Research (AWI): ePIC (electronic Publication Information Center) Arctic Greenland Harmful Algae 55 85 96 |
institution |
Open Polar |
collection |
Alfred Wegener Institute for Polar- and Marine Research (AWI): ePIC (electronic Publication Information Center) |
op_collection_id |
ftawi |
language |
unknown |
description |
Harmful algal blooms are mainly caused by marine dinoflagellates and are known to produce potent toxins that may affect the ecosystem, human activities and health. Such events have increased in frequency and intensity worldwide in the past decades. Numerous processes involved in Global Change are amplified in the Arctic, but little is known about species specific responses of arctic dinoflagellates. The aim of this work was to perform an exhaustive morphological, phylogenetical and toxinological characterization of Greenland Protoceratium reticulatum and, in addition, to test the effect of temperature on growth and production of bioactive secondary metabolites. Seven clonal isolates, the first isolates of P. reticulatum available from arctic waters, were phylogenetically characterized by analysis of the LSU rDNA. Six isolates were further characterized morphologically and were shown to produce both yessotoxins (YTX) and lytic compounds, representing the first report of allelochemical activity in P. reticulatum. As shown for one of the isolates, growth was strongly affected by temperature with a maximum growth rate at 15 8C, a significant but slow growth at 1 8C, and cell death at 25 8C, suggesting an adaptation of P. reticulatum to temperate waters. Temperature had no major effect on total YTX cell quota or lytic activity but both were affected by the growth phase with a significant increase at stationary phase. A comparison of six isolates at a fixed temperature of 10 8C showed high intraspecific variability for all three physiological parameters tested. Growth rate varied from 0.06 to 0.19 d�1, and total YTX concentration ranged from 0.3 to 15.0 pg YTX cell�1 and from 0.5 to 31.0 pg YTX cell�1 at exponential and stationary phase, respectively. All six isolates performed lytic activity; however, for two isolates lytic activity was only detectable at higher cell densities in stationary phase. |
format |
Article in Journal/Newspaper |
author |
Sala-Pérez, Manuel Alpermann, Tilman Krock, Bernd Tillmann, Urban |
spellingShingle |
Sala-Pérez, Manuel Alpermann, Tilman Krock, Bernd Tillmann, Urban Growth and bioactive secondary metabolites of arctic Protoceratium reticulatum (Dinophyceae) |
author_facet |
Sala-Pérez, Manuel Alpermann, Tilman Krock, Bernd Tillmann, Urban |
author_sort |
Sala-Pérez, Manuel |
title |
Growth and bioactive secondary metabolites of arctic Protoceratium reticulatum (Dinophyceae) |
title_short |
Growth and bioactive secondary metabolites of arctic Protoceratium reticulatum (Dinophyceae) |
title_full |
Growth and bioactive secondary metabolites of arctic Protoceratium reticulatum (Dinophyceae) |
title_fullStr |
Growth and bioactive secondary metabolites of arctic Protoceratium reticulatum (Dinophyceae) |
title_full_unstemmed |
Growth and bioactive secondary metabolites of arctic Protoceratium reticulatum (Dinophyceae) |
title_sort |
growth and bioactive secondary metabolites of arctic protoceratium reticulatum (dinophyceae) |
publisher |
ELSEVIER SCIENCE BV |
publishDate |
2016 |
url |
https://epic.awi.de/id/eprint/40227/ https://epic.awi.de/id/eprint/40227/1/2016_Sala_Perez_HA.pdf https://hdl.handle.net/10013/epic.47331 https://hdl.handle.net/10013/epic.47331.d001 |
geographic |
Arctic Greenland |
geographic_facet |
Arctic Greenland |
genre |
Arctic Arctic Greenland |
genre_facet |
Arctic Arctic Greenland |
op_source |
EPIC3Harmful Algae, ELSEVIER SCIENCE BV, 55, pp. 85-96, ISSN: 1568-9883 |
op_relation |
https://epic.awi.de/id/eprint/40227/1/2016_Sala_Perez_HA.pdf https://hdl.handle.net/10013/epic.47331.d001 Sala-Pérez, M. , Alpermann, T. , Krock, B. orcid:0000-0003-4022-9101 and Tillmann, U. orcid:0000-0002-8207-4382 (2016) Growth and bioactive secondary metabolites of arctic Protoceratium reticulatum (Dinophyceae) , Harmful Algae, 55 , pp. 85-96 . doi:10.1016/j.hal.2016.02.004 <https://doi.org/10.1016/j.hal.2016.02.004> , hdl:10013/epic.47331 |
op_doi |
https://doi.org/10.1016/j.hal.2016.02.004 |
container_title |
Harmful Algae |
container_volume |
55 |
container_start_page |
85 |
op_container_end_page |
96 |
_version_ |
1766300897478967296 |