Blue Blood on Ice: Cephalopod haemocyanin function and evolution in a latitudinal cline

The Southern Ocean hosts a rich and diverse fauna that required specialist adaptations to colonize and persist at temperatures close to freezing. While much has been revealed about key adaptations in Antarctic fishes little is known about evolutionary strategies of other Antarctic ectotherms, partic...

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Main Author: Oellermann, Michael
Format: Thesis
Language:unknown
Published: Bremen University 2015
Subjects:
Online Access:https://epic.awi.de/id/eprint/38062/
https://epic.awi.de/id/eprint/38062/1/PhDThesisMichaelOellermann2014.pdf
http://nbn-resolving.de/urn:nbn:de:gbv:46-00104376-17
https://hdl.handle.net/10013/epic.45615
https://hdl.handle.net/10013/epic.45615.d001
id ftawi:oai:epic.awi.de:38062
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spelling ftawi:oai:epic.awi.de:38062 2024-09-15T17:41:08+00:00 Blue Blood on Ice: Cephalopod haemocyanin function and evolution in a latitudinal cline Oellermann, Michael 2015-02-04 application/pdf https://epic.awi.de/id/eprint/38062/ https://epic.awi.de/id/eprint/38062/1/PhDThesisMichaelOellermann2014.pdf http://nbn-resolving.de/urn:nbn:de:gbv:46-00104376-17 https://hdl.handle.net/10013/epic.45615 https://hdl.handle.net/10013/epic.45615.d001 unknown Bremen University https://epic.awi.de/id/eprint/38062/1/PhDThesisMichaelOellermann2014.pdf https://hdl.handle.net/10013/epic.45615.d001 Oellermann, M. (2015) Blue Blood on Ice: Cephalopod haemocyanin function and evolution in a latitudinal cline , PhD thesis, Bremen University. doi:10.13140/RG.2.1.1283.3442 <https://doi.org/10.13140/RG.2.1.1283.3442> , hdl:10013/epic.45615 EPIC3Bremen University, 213 p. Thesis notRev 2015 ftawi https://doi.org/10.13140/RG.2.1.1283.3442 2024-06-24T04:12:21Z The Southern Ocean hosts a rich and diverse fauna that required specialist adaptations to colonize and persist at temperatures close to freezing. While much has been revealed about key adaptations in Antarctic fishes little is known about evolutionary strategies of other Antarctic ectotherms, particularly the abundant benthic incirrate octopods. Their oxygen demand is largely fuelled by the blue oxygen transporter haemocyanin that however, due to its increasing functional failure towards colder temperatures, poses a prime target for cold-adaptive adjustment. While haemocyanin structure has been well understood it remains unclear which molecular features and evolutionary trajectories explain functional properties of octopod haemocyanin. This thesis thus aimed to unravel cold-adaptive features of octopod haemocyanin and the underlying molecular features that evolved to sustain oxygen supply at sub-zero temperatures. Haemocyanin function is best assessed by oxygen binding experiments, which however was challenged due to the minute haemolymph volumes non-model organisms like Antarctic octopods, yield. I thus upgraded an oxygen diffusion chamber with a broad range fibre optic spectrophotometer and a micro-pH optode and tested the setup for Octopus vulgaris, the Antarctic eelpout and a Baikal amphipod. This technical advancement enabled simultaneous recordings of pH and oxygen dependent pigment absorbance in only 15 µl of sample. Results were highly reproducible and accurate and provided detailed insight to the complex and dynamic spectral features of three diverse blood-types. To identify cold-adaptive functional traits of blood oxygen transport this study compared haemocyanin oxygen binding properties, oxygen carrying capacities and haemolymph protein and ion composition between the Antarctic octopod Pareledone charcoti, the temperate Octopus pallidus and the subtropical Eledone moschata. Compared to octopods from warmer climates, Pareledone charcoti showed incomplete but significantly reduced oxygen affinity, which ... Thesis Antarc* Antarctic Southern Ocean Alfred Wegener Institute for Polar- and Marine Research (AWI): ePIC (electronic Publication Information Center)
institution Open Polar
collection Alfred Wegener Institute for Polar- and Marine Research (AWI): ePIC (electronic Publication Information Center)
op_collection_id ftawi
language unknown
description The Southern Ocean hosts a rich and diverse fauna that required specialist adaptations to colonize and persist at temperatures close to freezing. While much has been revealed about key adaptations in Antarctic fishes little is known about evolutionary strategies of other Antarctic ectotherms, particularly the abundant benthic incirrate octopods. Their oxygen demand is largely fuelled by the blue oxygen transporter haemocyanin that however, due to its increasing functional failure towards colder temperatures, poses a prime target for cold-adaptive adjustment. While haemocyanin structure has been well understood it remains unclear which molecular features and evolutionary trajectories explain functional properties of octopod haemocyanin. This thesis thus aimed to unravel cold-adaptive features of octopod haemocyanin and the underlying molecular features that evolved to sustain oxygen supply at sub-zero temperatures. Haemocyanin function is best assessed by oxygen binding experiments, which however was challenged due to the minute haemolymph volumes non-model organisms like Antarctic octopods, yield. I thus upgraded an oxygen diffusion chamber with a broad range fibre optic spectrophotometer and a micro-pH optode and tested the setup for Octopus vulgaris, the Antarctic eelpout and a Baikal amphipod. This technical advancement enabled simultaneous recordings of pH and oxygen dependent pigment absorbance in only 15 µl of sample. Results were highly reproducible and accurate and provided detailed insight to the complex and dynamic spectral features of three diverse blood-types. To identify cold-adaptive functional traits of blood oxygen transport this study compared haemocyanin oxygen binding properties, oxygen carrying capacities and haemolymph protein and ion composition between the Antarctic octopod Pareledone charcoti, the temperate Octopus pallidus and the subtropical Eledone moschata. Compared to octopods from warmer climates, Pareledone charcoti showed incomplete but significantly reduced oxygen affinity, which ...
format Thesis
author Oellermann, Michael
spellingShingle Oellermann, Michael
Blue Blood on Ice: Cephalopod haemocyanin function and evolution in a latitudinal cline
author_facet Oellermann, Michael
author_sort Oellermann, Michael
title Blue Blood on Ice: Cephalopod haemocyanin function and evolution in a latitudinal cline
title_short Blue Blood on Ice: Cephalopod haemocyanin function and evolution in a latitudinal cline
title_full Blue Blood on Ice: Cephalopod haemocyanin function and evolution in a latitudinal cline
title_fullStr Blue Blood on Ice: Cephalopod haemocyanin function and evolution in a latitudinal cline
title_full_unstemmed Blue Blood on Ice: Cephalopod haemocyanin function and evolution in a latitudinal cline
title_sort blue blood on ice: cephalopod haemocyanin function and evolution in a latitudinal cline
publisher Bremen University
publishDate 2015
url https://epic.awi.de/id/eprint/38062/
https://epic.awi.de/id/eprint/38062/1/PhDThesisMichaelOellermann2014.pdf
http://nbn-resolving.de/urn:nbn:de:gbv:46-00104376-17
https://hdl.handle.net/10013/epic.45615
https://hdl.handle.net/10013/epic.45615.d001
genre Antarc*
Antarctic
Southern Ocean
genre_facet Antarc*
Antarctic
Southern Ocean
op_source EPIC3Bremen University, 213 p.
op_relation https://epic.awi.de/id/eprint/38062/1/PhDThesisMichaelOellermann2014.pdf
https://hdl.handle.net/10013/epic.45615.d001
Oellermann, M. (2015) Blue Blood on Ice: Cephalopod haemocyanin function and evolution in a latitudinal cline , PhD thesis, Bremen University. doi:10.13140/RG.2.1.1283.3442 <https://doi.org/10.13140/RG.2.1.1283.3442> , hdl:10013/epic.45615
op_doi https://doi.org/10.13140/RG.2.1.1283.3442
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