Pyrosequencing and de novo assembly of Antarctic krill (Euphausia superba) transcriptome to study the adaptability of krill to climate-induced environmental changes
The Antarctic krill, Euphausia superba, has a key position in the Southern Ocean food web by serving as direct link between primary producers and apex predators. The southwest Atlantic sector of the Southern Ocean, where the majority of the krill population is located, is experiencing one of the mos...
Published in: | Molecular Ecology Resources |
---|---|
Main Authors: | , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | unknown |
Published: |
WILEY-BLACKWELL PUBLISHING
2015
|
Subjects: | |
Online Access: | https://epic.awi.de/id/eprint/37736/ https://epic.awi.de/id/eprint/37736/1/Meyer_et_al-2015-Molecular_Ecology_Resources.pdf https://hdl.handle.net/10013/epic.45360 https://hdl.handle.net/10013/epic.45360.d001 |
id |
ftawi:oai:epic.awi.de:37736 |
---|---|
record_format |
openpolar |
spelling |
ftawi:oai:epic.awi.de:37736 2023-05-15T13:40:27+02:00 Pyrosequencing and de novo assembly of Antarctic krill (Euphausia superba) transcriptome to study the adaptability of krill to climate-induced environmental changes Meyer, Bettina Martini, Paolo Biscontin, Alberto DePitta, Cristiano Romualdi, Ciara Teschke, Mathias Frickenhaus, Stephan Harms, Lars Freier, Ulrich Jarman, Simon Kawaguchi, So 2015-03-18 application/pdf https://epic.awi.de/id/eprint/37736/ https://epic.awi.de/id/eprint/37736/1/Meyer_et_al-2015-Molecular_Ecology_Resources.pdf https://hdl.handle.net/10013/epic.45360 https://hdl.handle.net/10013/epic.45360.d001 unknown WILEY-BLACKWELL PUBLISHING https://epic.awi.de/id/eprint/37736/1/Meyer_et_al-2015-Molecular_Ecology_Resources.pdf https://hdl.handle.net/10013/epic.45360.d001 Meyer, B. orcid:0000-0001-6804-9896 , Martini, P. , Biscontin, A. , DePitta, C. , Romualdi, C. , Teschke, M. , Frickenhaus, S. orcid:0000-0002-0356-9791 , Harms, L. orcid:0000-0001-7620-0613 , Freier, U. , Jarman, S. and Kawaguchi, S. (2015) Pyrosequencing and de novo assembly of Antarctic krill (Euphausia superba) transcriptome to study the adaptability of krill to climate-induced environmental changes , Molecular Ecology Resources . doi:10.1111/1755-0998.12408 <https://doi.org/10.1111/1755-0998.12408> , hdl:10013/epic.45360 EPIC3Molecular Ecology Resources, WILEY-BLACKWELL PUBLISHING, ISSN: 1755-098X Article peerRev 2015 ftawi https://doi.org/10.1111/1755-0998.12408 2021-12-24T15:40:23Z The Antarctic krill, Euphausia superba, has a key position in the Southern Ocean food web by serving as direct link between primary producers and apex predators. The southwest Atlantic sector of the Southern Ocean, where the majority of the krill population is located, is experiencing one of the most profound environmental changes worldwide. Up to now, we have only cursory information about krill’s genomic plasticity to cope with the ongoing environmental changes induced by anthropogenic CO2 emission. The genome of krill is not yet available due to its large size (about 48 Gbp). Here, we present two cDNA normalized libraries from whole krill and krill heads sampled in different seasons that were combined with two datasets of krill transcriptome projects, already published, in order to produce the first knowledgebase krill ‘master’ transcriptome. The new library produced 25% more E. superba transcripts and now includes nearly all the enzymes involved in the primary oxidative metabolism (Glycolysis, Krebs cycle and oxidative phosphorylation) as well as all genes involved in glycogenesis, glycogen breakdown, gluconeogenesis, fatty acid synthesis and fatty acids β-oxidation. With these features, the ‘master’ transcriptome provides the most complete picture of metabolic pathways in Antarctic krill and will provide a major resource for future physiological and molecular studies. This will be particularly valuable for characterizing the molecular networks that respond to stressors caused by the anthropogenic CO2 emissions and krill’s capacity to cope with the ongoing environmental changes in the Atlantic sector of the Southern Ocean. Article in Journal/Newspaper Antarc* Antarctic Antarctic Krill Euphausia superba Southern Ocean Alfred Wegener Institute for Polar- and Marine Research (AWI): ePIC (electronic Publication Information Center) Antarctic Krebs ENVELOPE(-61.467,-61.467,-64.633,-64.633) Southern Ocean The Antarctic Molecular Ecology Resources 15 6 1460 1471 |
institution |
Open Polar |
collection |
Alfred Wegener Institute for Polar- and Marine Research (AWI): ePIC (electronic Publication Information Center) |
op_collection_id |
ftawi |
language |
unknown |
description |
The Antarctic krill, Euphausia superba, has a key position in the Southern Ocean food web by serving as direct link between primary producers and apex predators. The southwest Atlantic sector of the Southern Ocean, where the majority of the krill population is located, is experiencing one of the most profound environmental changes worldwide. Up to now, we have only cursory information about krill’s genomic plasticity to cope with the ongoing environmental changes induced by anthropogenic CO2 emission. The genome of krill is not yet available due to its large size (about 48 Gbp). Here, we present two cDNA normalized libraries from whole krill and krill heads sampled in different seasons that were combined with two datasets of krill transcriptome projects, already published, in order to produce the first knowledgebase krill ‘master’ transcriptome. The new library produced 25% more E. superba transcripts and now includes nearly all the enzymes involved in the primary oxidative metabolism (Glycolysis, Krebs cycle and oxidative phosphorylation) as well as all genes involved in glycogenesis, glycogen breakdown, gluconeogenesis, fatty acid synthesis and fatty acids β-oxidation. With these features, the ‘master’ transcriptome provides the most complete picture of metabolic pathways in Antarctic krill and will provide a major resource for future physiological and molecular studies. This will be particularly valuable for characterizing the molecular networks that respond to stressors caused by the anthropogenic CO2 emissions and krill’s capacity to cope with the ongoing environmental changes in the Atlantic sector of the Southern Ocean. |
format |
Article in Journal/Newspaper |
author |
Meyer, Bettina Martini, Paolo Biscontin, Alberto DePitta, Cristiano Romualdi, Ciara Teschke, Mathias Frickenhaus, Stephan Harms, Lars Freier, Ulrich Jarman, Simon Kawaguchi, So |
spellingShingle |
Meyer, Bettina Martini, Paolo Biscontin, Alberto DePitta, Cristiano Romualdi, Ciara Teschke, Mathias Frickenhaus, Stephan Harms, Lars Freier, Ulrich Jarman, Simon Kawaguchi, So Pyrosequencing and de novo assembly of Antarctic krill (Euphausia superba) transcriptome to study the adaptability of krill to climate-induced environmental changes |
author_facet |
Meyer, Bettina Martini, Paolo Biscontin, Alberto DePitta, Cristiano Romualdi, Ciara Teschke, Mathias Frickenhaus, Stephan Harms, Lars Freier, Ulrich Jarman, Simon Kawaguchi, So |
author_sort |
Meyer, Bettina |
title |
Pyrosequencing and de novo assembly of Antarctic krill (Euphausia superba) transcriptome to study the adaptability of krill to climate-induced environmental changes |
title_short |
Pyrosequencing and de novo assembly of Antarctic krill (Euphausia superba) transcriptome to study the adaptability of krill to climate-induced environmental changes |
title_full |
Pyrosequencing and de novo assembly of Antarctic krill (Euphausia superba) transcriptome to study the adaptability of krill to climate-induced environmental changes |
title_fullStr |
Pyrosequencing and de novo assembly of Antarctic krill (Euphausia superba) transcriptome to study the adaptability of krill to climate-induced environmental changes |
title_full_unstemmed |
Pyrosequencing and de novo assembly of Antarctic krill (Euphausia superba) transcriptome to study the adaptability of krill to climate-induced environmental changes |
title_sort |
pyrosequencing and de novo assembly of antarctic krill (euphausia superba) transcriptome to study the adaptability of krill to climate-induced environmental changes |
publisher |
WILEY-BLACKWELL PUBLISHING |
publishDate |
2015 |
url |
https://epic.awi.de/id/eprint/37736/ https://epic.awi.de/id/eprint/37736/1/Meyer_et_al-2015-Molecular_Ecology_Resources.pdf https://hdl.handle.net/10013/epic.45360 https://hdl.handle.net/10013/epic.45360.d001 |
long_lat |
ENVELOPE(-61.467,-61.467,-64.633,-64.633) |
geographic |
Antarctic Krebs Southern Ocean The Antarctic |
geographic_facet |
Antarctic Krebs Southern Ocean The Antarctic |
genre |
Antarc* Antarctic Antarctic Krill Euphausia superba Southern Ocean |
genre_facet |
Antarc* Antarctic Antarctic Krill Euphausia superba Southern Ocean |
op_source |
EPIC3Molecular Ecology Resources, WILEY-BLACKWELL PUBLISHING, ISSN: 1755-098X |
op_relation |
https://epic.awi.de/id/eprint/37736/1/Meyer_et_al-2015-Molecular_Ecology_Resources.pdf https://hdl.handle.net/10013/epic.45360.d001 Meyer, B. orcid:0000-0001-6804-9896 , Martini, P. , Biscontin, A. , DePitta, C. , Romualdi, C. , Teschke, M. , Frickenhaus, S. orcid:0000-0002-0356-9791 , Harms, L. orcid:0000-0001-7620-0613 , Freier, U. , Jarman, S. and Kawaguchi, S. (2015) Pyrosequencing and de novo assembly of Antarctic krill (Euphausia superba) transcriptome to study the adaptability of krill to climate-induced environmental changes , Molecular Ecology Resources . doi:10.1111/1755-0998.12408 <https://doi.org/10.1111/1755-0998.12408> , hdl:10013/epic.45360 |
op_doi |
https://doi.org/10.1111/1755-0998.12408 |
container_title |
Molecular Ecology Resources |
container_volume |
15 |
container_issue |
6 |
container_start_page |
1460 |
op_container_end_page |
1471 |
_version_ |
1766134497965768704 |