A Unique Capsular Polysaccharide Structure from the Psychrophilic Marine Bacterium Colwellia psychrerythraea 34H That Mimics Antifreeze (Glyco)proteins
The low temperatures of polar regions and high-altitude environments, especially icy habitats, present challenges for many microorganisms. Their ability to live under subfreezing conditions implies the production of compounds conferring cryotolerance. Colwellia psychrerythraea 34H, a γ-proteobacteri...
Published in: | Journal of the American Chemical Society |
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American Chemical Society
2015
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Online Access: | https://epic.awi.de/id/eprint/37118/ https://epic.awi.de/id/eprint/37118/1/Manuscript_inviato.pdf http://pubs.acs.org/articlesonrequest/AOR-aCx4z5bcMwRjfxUFsDfm https://hdl.handle.net/10013/epic.44844 https://hdl.handle.net/10013/epic.44844.d001 |
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ftawi:oai:epic.awi.de:37118 2023-05-15T15:05:09+02:00 A Unique Capsular Polysaccharide Structure from the Psychrophilic Marine Bacterium Colwellia psychrerythraea 34H That Mimics Antifreeze (Glyco)proteins Carillo, Sara Casillo, Angela Pieretti, Giuseppina Parrilli, Ermenegilda Sannino, Filomena Bayer-Giraldi, Maddalena Cosconati, Sandro Novellino, Ettore Ewert, Marcela Deming, Jody W. Lanzetta, Rosa Marino, Gennaro Parrilli, Michelangelo Randazzo, Antonio Tutino, Maria Luisa Corsaro, Maria Michela 2015 application/pdf https://epic.awi.de/id/eprint/37118/ https://epic.awi.de/id/eprint/37118/1/Manuscript_inviato.pdf http://pubs.acs.org/articlesonrequest/AOR-aCx4z5bcMwRjfxUFsDfm https://hdl.handle.net/10013/epic.44844 https://hdl.handle.net/10013/epic.44844.d001 unknown American Chemical Society https://epic.awi.de/id/eprint/37118/1/Manuscript_inviato.pdf https://hdl.handle.net/10013/epic.44844.d001 Carillo, S. , Casillo, A. , Pieretti, G. , Parrilli, E. , Sannino, F. , Bayer-Giraldi, M. orcid:0000-0002-7158-5478 , Cosconati, S. , Novellino, E. , Ewert, M. , Deming, J. W. , Lanzetta, R. , Marino, G. , Parrilli, M. , Randazzo, A. , Tutino, M. L. and Corsaro, M. M. (2015) A Unique Capsular Polysaccharide Structure from the Psychrophilic Marine Bacterium Colwellia psychrerythraea 34H That Mimics Antifreeze (Glyco)proteins , Journal of the American Chemical Society, 137 (1), pp. 179-189 . doi:10.1021/ja5075954 <https://doi.org/10.1021/ja5075954> , hdl:10013/epic.44844 EPIC3Journal of the American Chemical Society, American Chemical Society, 137(1), pp. 179-189, ISSN: 0002-7863 Article isiRev 2015 ftawi https://doi.org/10.1021/ja5075954 2021-12-24T15:40:09Z The low temperatures of polar regions and high-altitude environments, especially icy habitats, present challenges for many microorganisms. Their ability to live under subfreezing conditions implies the production of compounds conferring cryotolerance. Colwellia psychrerythraea 34H, a γ-proteobacterium isolated from subzero Arctic marine sediments, provides a model for the study of life in cold environments. We report here the identification and detailed molecular primary and secondary structures of capsular polysaccharide from C. psychrerythraea 34H cells. The polymer was isolated in the water layer when cells were extracted by phenol/water and characterized by one- and two-dimensional NMR spectroscopy together with chemical analysis. Molecular mechanics and dynamics calculations were also performed. The polysaccharide consists of a tetrasaccharidic repeating unit containing two amino sugars and two uronic acids bearing threonine as substituent. The structural features of this unique polysaccharide resemble those present in antifreeze proteins and glycoproteins. These results suggest a possible correlation between the capsule structure and the ability of C. psychrerythraea to colonize subfreezing marine environments. Article in Journal/Newspaper Arctic Alfred Wegener Institute for Polar- and Marine Research (AWI): ePIC (electronic Publication Information Center) Arctic Journal of the American Chemical Society 137 1 179 189 |
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Alfred Wegener Institute for Polar- and Marine Research (AWI): ePIC (electronic Publication Information Center) |
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ftawi |
language |
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description |
The low temperatures of polar regions and high-altitude environments, especially icy habitats, present challenges for many microorganisms. Their ability to live under subfreezing conditions implies the production of compounds conferring cryotolerance. Colwellia psychrerythraea 34H, a γ-proteobacterium isolated from subzero Arctic marine sediments, provides a model for the study of life in cold environments. We report here the identification and detailed molecular primary and secondary structures of capsular polysaccharide from C. psychrerythraea 34H cells. The polymer was isolated in the water layer when cells were extracted by phenol/water and characterized by one- and two-dimensional NMR spectroscopy together with chemical analysis. Molecular mechanics and dynamics calculations were also performed. The polysaccharide consists of a tetrasaccharidic repeating unit containing two amino sugars and two uronic acids bearing threonine as substituent. The structural features of this unique polysaccharide resemble those present in antifreeze proteins and glycoproteins. These results suggest a possible correlation between the capsule structure and the ability of C. psychrerythraea to colonize subfreezing marine environments. |
format |
Article in Journal/Newspaper |
author |
Carillo, Sara Casillo, Angela Pieretti, Giuseppina Parrilli, Ermenegilda Sannino, Filomena Bayer-Giraldi, Maddalena Cosconati, Sandro Novellino, Ettore Ewert, Marcela Deming, Jody W. Lanzetta, Rosa Marino, Gennaro Parrilli, Michelangelo Randazzo, Antonio Tutino, Maria Luisa Corsaro, Maria Michela |
spellingShingle |
Carillo, Sara Casillo, Angela Pieretti, Giuseppina Parrilli, Ermenegilda Sannino, Filomena Bayer-Giraldi, Maddalena Cosconati, Sandro Novellino, Ettore Ewert, Marcela Deming, Jody W. Lanzetta, Rosa Marino, Gennaro Parrilli, Michelangelo Randazzo, Antonio Tutino, Maria Luisa Corsaro, Maria Michela A Unique Capsular Polysaccharide Structure from the Psychrophilic Marine Bacterium Colwellia psychrerythraea 34H That Mimics Antifreeze (Glyco)proteins |
author_facet |
Carillo, Sara Casillo, Angela Pieretti, Giuseppina Parrilli, Ermenegilda Sannino, Filomena Bayer-Giraldi, Maddalena Cosconati, Sandro Novellino, Ettore Ewert, Marcela Deming, Jody W. Lanzetta, Rosa Marino, Gennaro Parrilli, Michelangelo Randazzo, Antonio Tutino, Maria Luisa Corsaro, Maria Michela |
author_sort |
Carillo, Sara |
title |
A Unique Capsular Polysaccharide Structure from the Psychrophilic Marine Bacterium Colwellia psychrerythraea 34H That Mimics Antifreeze (Glyco)proteins |
title_short |
A Unique Capsular Polysaccharide Structure from the Psychrophilic Marine Bacterium Colwellia psychrerythraea 34H That Mimics Antifreeze (Glyco)proteins |
title_full |
A Unique Capsular Polysaccharide Structure from the Psychrophilic Marine Bacterium Colwellia psychrerythraea 34H That Mimics Antifreeze (Glyco)proteins |
title_fullStr |
A Unique Capsular Polysaccharide Structure from the Psychrophilic Marine Bacterium Colwellia psychrerythraea 34H That Mimics Antifreeze (Glyco)proteins |
title_full_unstemmed |
A Unique Capsular Polysaccharide Structure from the Psychrophilic Marine Bacterium Colwellia psychrerythraea 34H That Mimics Antifreeze (Glyco)proteins |
title_sort |
unique capsular polysaccharide structure from the psychrophilic marine bacterium colwellia psychrerythraea 34h that mimics antifreeze (glyco)proteins |
publisher |
American Chemical Society |
publishDate |
2015 |
url |
https://epic.awi.de/id/eprint/37118/ https://epic.awi.de/id/eprint/37118/1/Manuscript_inviato.pdf http://pubs.acs.org/articlesonrequest/AOR-aCx4z5bcMwRjfxUFsDfm https://hdl.handle.net/10013/epic.44844 https://hdl.handle.net/10013/epic.44844.d001 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
EPIC3Journal of the American Chemical Society, American Chemical Society, 137(1), pp. 179-189, ISSN: 0002-7863 |
op_relation |
https://epic.awi.de/id/eprint/37118/1/Manuscript_inviato.pdf https://hdl.handle.net/10013/epic.44844.d001 Carillo, S. , Casillo, A. , Pieretti, G. , Parrilli, E. , Sannino, F. , Bayer-Giraldi, M. orcid:0000-0002-7158-5478 , Cosconati, S. , Novellino, E. , Ewert, M. , Deming, J. W. , Lanzetta, R. , Marino, G. , Parrilli, M. , Randazzo, A. , Tutino, M. L. and Corsaro, M. M. (2015) A Unique Capsular Polysaccharide Structure from the Psychrophilic Marine Bacterium Colwellia psychrerythraea 34H That Mimics Antifreeze (Glyco)proteins , Journal of the American Chemical Society, 137 (1), pp. 179-189 . doi:10.1021/ja5075954 <https://doi.org/10.1021/ja5075954> , hdl:10013/epic.44844 |
op_doi |
https://doi.org/10.1021/ja5075954 |
container_title |
Journal of the American Chemical Society |
container_volume |
137 |
container_issue |
1 |
container_start_page |
179 |
op_container_end_page |
189 |
_version_ |
1766336911523184640 |