Gene expression profiling in gills of the great spider crab Hyas araneus in response to ocean acidification and warming
Background Hypercapnia and elevated temperatures resulting from climate change may have adverse consequences for many marine organisms. While diverse physiological and ecological effects have been identified, changes in those molecular mechanisms, which shape the physiological phenotype of a species...
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Online Access: | https://epic.awi.de/id/eprint/36295/ https://epic.awi.de/id/eprint/36295/1/1471-2164-15-789.pdf http://www.biomedcentral.com/1471-2164/15/789 https://hdl.handle.net/10013/epic.44140 https://hdl.handle.net/10013/epic.44140.d001 |
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ftawi:oai:epic.awi.de:36295 2023-05-15T17:50:12+02:00 Gene expression profiling in gills of the great spider crab Hyas araneus in response to ocean acidification and warming Harms, Lars Frickenhaus, Stephan Schiffer, Melanie Mark, Felix Christopher Storch, Daniela Held, Christoph Pörtner, Hans-Otto Lucassen, Magnus 2014-09-12 application/pdf https://epic.awi.de/id/eprint/36295/ https://epic.awi.de/id/eprint/36295/1/1471-2164-15-789.pdf http://www.biomedcentral.com/1471-2164/15/789 https://hdl.handle.net/10013/epic.44140 https://hdl.handle.net/10013/epic.44140.d001 unknown BMC https://epic.awi.de/id/eprint/36295/1/1471-2164-15-789.pdf https://hdl.handle.net/10013/epic.44140.d001 Harms, L. orcid:0000-0001-7620-0613 , Frickenhaus, S. orcid:0000-0002-0356-9791 , Schiffer, M. , Mark, F. C. orcid:0000-0002-5586-6704 , Storch, D. orcid:0000-0003-3090-7554 , Held, C. orcid:0000-0001-8854-3234 , Pörtner, H. O. orcid:0000-0001-6535-6575 and Lucassen, M. orcid:0000-0003-4276-4781 (2014) Gene expression profiling in gills of the great spider crab Hyas araneus in response to ocean acidification and warming , BMC Genomics, 15 (1), p. 789 . doi:10.1186/1471-2164-15-789 <https://doi.org/10.1186/1471-2164-15-789> , hdl:10013/epic.44140 EPIC3BMC Genomics, BMC, 15(1), pp. 789, ISSN: 1471-2164 Article isiRev 2014 ftawi https://doi.org/10.1186/1471-2164-15-789 2021-12-24T15:39:49Z Background Hypercapnia and elevated temperatures resulting from climate change may have adverse consequences for many marine organisms. While diverse physiological and ecological effects have been identified, changes in those molecular mechanisms, which shape the physiological phenotype of a species and limit its capacity to compensate, remain poorly understood. Here, we use global gene expression profiling through RNA-Sequencing to study the transcriptional responses to ocean acidification and warming in gills of the boreal spider crab Hyas araneus exposed medium-term (10 weeks) to intermediate (1,120 μatm) and high (1,960 μatm) PCO2 at different temperatures (5°C and 10°C). Results The analyses reveal shifts in steady state gene expression from control to intermediate and from intermediate to high CO2 exposures. At 5°C acid–base, energy metabolism and stress response related genes were upregulated at intermediate PCO2, whereas high PCO2 induced a relative reduction in expression to levels closer to controls. A similar pattern was found at elevated temperature (10°C). There was a strong coordination between acid–base, metabolic and stress-related processes. Hemolymph parameters at intermediate PCO2 indicate enhanced capacity in acid–base compensation potentially supported by upregulation of a V-ATPase. The likely enhanced energy demand might be met by the upregulation of the electron transport system (ETS), but may lead to increased oxidative stress reflected in upregulated antioxidant defense transcripts. These mechanisms were attenuated by high PCO2, possibly as a result of limited acid–base compensation and metabolic down-regulation. Conclusion Our findings indicate a PCO2 dependent threshold beyond which compensation by acclimation fails progressively. They also indicate a limited ability of this stenoecious crustacean to compensate for the effects of ocean acidification with and without concomitant warming. Article in Journal/Newspaper Ocean acidification Alfred Wegener Institute for Polar- and Marine Research (AWI): ePIC (electronic Publication Information Center) BMC Genomics 15 1 789 |
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Alfred Wegener Institute for Polar- and Marine Research (AWI): ePIC (electronic Publication Information Center) |
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description |
Background Hypercapnia and elevated temperatures resulting from climate change may have adverse consequences for many marine organisms. While diverse physiological and ecological effects have been identified, changes in those molecular mechanisms, which shape the physiological phenotype of a species and limit its capacity to compensate, remain poorly understood. Here, we use global gene expression profiling through RNA-Sequencing to study the transcriptional responses to ocean acidification and warming in gills of the boreal spider crab Hyas araneus exposed medium-term (10 weeks) to intermediate (1,120 μatm) and high (1,960 μatm) PCO2 at different temperatures (5°C and 10°C). Results The analyses reveal shifts in steady state gene expression from control to intermediate and from intermediate to high CO2 exposures. At 5°C acid–base, energy metabolism and stress response related genes were upregulated at intermediate PCO2, whereas high PCO2 induced a relative reduction in expression to levels closer to controls. A similar pattern was found at elevated temperature (10°C). There was a strong coordination between acid–base, metabolic and stress-related processes. Hemolymph parameters at intermediate PCO2 indicate enhanced capacity in acid–base compensation potentially supported by upregulation of a V-ATPase. The likely enhanced energy demand might be met by the upregulation of the electron transport system (ETS), but may lead to increased oxidative stress reflected in upregulated antioxidant defense transcripts. These mechanisms were attenuated by high PCO2, possibly as a result of limited acid–base compensation and metabolic down-regulation. Conclusion Our findings indicate a PCO2 dependent threshold beyond which compensation by acclimation fails progressively. They also indicate a limited ability of this stenoecious crustacean to compensate for the effects of ocean acidification with and without concomitant warming. |
format |
Article in Journal/Newspaper |
author |
Harms, Lars Frickenhaus, Stephan Schiffer, Melanie Mark, Felix Christopher Storch, Daniela Held, Christoph Pörtner, Hans-Otto Lucassen, Magnus |
spellingShingle |
Harms, Lars Frickenhaus, Stephan Schiffer, Melanie Mark, Felix Christopher Storch, Daniela Held, Christoph Pörtner, Hans-Otto Lucassen, Magnus Gene expression profiling in gills of the great spider crab Hyas araneus in response to ocean acidification and warming |
author_facet |
Harms, Lars Frickenhaus, Stephan Schiffer, Melanie Mark, Felix Christopher Storch, Daniela Held, Christoph Pörtner, Hans-Otto Lucassen, Magnus |
author_sort |
Harms, Lars |
title |
Gene expression profiling in gills of the great spider crab Hyas araneus in response to ocean acidification and warming |
title_short |
Gene expression profiling in gills of the great spider crab Hyas araneus in response to ocean acidification and warming |
title_full |
Gene expression profiling in gills of the great spider crab Hyas araneus in response to ocean acidification and warming |
title_fullStr |
Gene expression profiling in gills of the great spider crab Hyas araneus in response to ocean acidification and warming |
title_full_unstemmed |
Gene expression profiling in gills of the great spider crab Hyas araneus in response to ocean acidification and warming |
title_sort |
gene expression profiling in gills of the great spider crab hyas araneus in response to ocean acidification and warming |
publisher |
BMC |
publishDate |
2014 |
url |
https://epic.awi.de/id/eprint/36295/ https://epic.awi.de/id/eprint/36295/1/1471-2164-15-789.pdf http://www.biomedcentral.com/1471-2164/15/789 https://hdl.handle.net/10013/epic.44140 https://hdl.handle.net/10013/epic.44140.d001 |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_source |
EPIC3BMC Genomics, BMC, 15(1), pp. 789, ISSN: 1471-2164 |
op_relation |
https://epic.awi.de/id/eprint/36295/1/1471-2164-15-789.pdf https://hdl.handle.net/10013/epic.44140.d001 Harms, L. orcid:0000-0001-7620-0613 , Frickenhaus, S. orcid:0000-0002-0356-9791 , Schiffer, M. , Mark, F. C. orcid:0000-0002-5586-6704 , Storch, D. orcid:0000-0003-3090-7554 , Held, C. orcid:0000-0001-8854-3234 , Pörtner, H. O. orcid:0000-0001-6535-6575 and Lucassen, M. orcid:0000-0003-4276-4781 (2014) Gene expression profiling in gills of the great spider crab Hyas araneus in response to ocean acidification and warming , BMC Genomics, 15 (1), p. 789 . doi:10.1186/1471-2164-15-789 <https://doi.org/10.1186/1471-2164-15-789> , hdl:10013/epic.44140 |
op_doi |
https://doi.org/10.1186/1471-2164-15-789 |
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BMC Genomics |
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15 |
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789 |
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1766156863638863872 |