CO2-induced fertilization impairment in Strongylocentrotus droebachiensis collected in the Arctic
Fertilization depends on distribution and aggregation patterns of sea urchins which influence gamete contact time and may potentially enhance their vulnerability to ocean acidification. In this study, we conducted fertilization experiments to assess the effects of selected pH scenarios on fertilizat...
Published in: | Helgoland Marine Research |
---|---|
Main Authors: | , , |
Format: | Article in Journal/Newspaper |
Language: | unknown |
Published: |
SPRINGER
2014
|
Subjects: | |
Online Access: | https://epic.awi.de/id/eprint/35703/ https://epic.awi.de/id/eprint/35703/1/BoegnerBickmeyerKoehlerHMR2014.pdf https://hdl.handle.net/10013/epic.43637 https://hdl.handle.net/10013/epic.43637.d001 |
id |
ftawi:oai:epic.awi.de:35703 |
---|---|
record_format |
openpolar |
spelling |
ftawi:oai:epic.awi.de:35703 2024-05-12T07:57:35+00:00 CO2-induced fertilization impairment in Strongylocentrotus droebachiensis collected in the Arctic Bögner, Desislava Bickmeyer, Ulf Köhler, Angela 2014-06 application/pdf https://epic.awi.de/id/eprint/35703/ https://epic.awi.de/id/eprint/35703/1/BoegnerBickmeyerKoehlerHMR2014.pdf https://hdl.handle.net/10013/epic.43637 https://hdl.handle.net/10013/epic.43637.d001 unknown SPRINGER https://epic.awi.de/id/eprint/35703/1/BoegnerBickmeyerKoehlerHMR2014.pdf https://hdl.handle.net/10013/epic.43637.d001 Bögner, D. orcid:0000-0002-8318-8568 , Bickmeyer, U. orcid:0000-0002-5351-2902 and Köhler, A. (2014) CO2-induced fertilization impairment in Strongylocentrotus droebachiensis collected in the Arctic , Helgoland Marine Research, 68 (2), pp. 341-356 . doi:10.1007/s10152-014-0394-3 <https://doi.org/10.1007/s10152-014-0394-3> , hdl:10013/epic.43637 EPIC3Helgoland Marine Research, SPRINGER, 68(2), pp. 341-356, ISSN: 1438-387X Article isiRev 2014 ftawi https://doi.org/10.1007/s10152-014-0394-3 2024-04-17T14:05:02Z Fertilization depends on distribution and aggregation patterns of sea urchins which influence gamete contact time and may potentially enhance their vulnerability to ocean acidification. In this study, we conducted fertilization experiments to assess the effects of selected pH scenarios on fertilization success of Strongylocentrotus droebachiensis, from Spitsbergen, Arctic. Acidification was achieved by aerating seawater with different CO2 partial pressures to represent preindustrial and present conditions (measured*180–425 latm) and future acidification scenarios (*550–800, *1,300, *2,000 latm). Fertilization success was defined as the proportion of successful/unsuccessful fertilizations per treatment; eggs were classified according to features of their fertilization envelope (FE), hyaline layer (HL) and achievement of cellular division. The diagnostic findings of specific pathological aberrations were described in detail. We additionally measured intracellular pH changes in unfertilized eggs exposed for 1 h to selected acidification treatments using BCECF/AM. We conclude that (a) acidified conditions increase the proportion of eggs that failed fertilization, (b) acidification may increase the risk of polyspermy due to failures in the FE formation supported by the occasional observation ofmultiple sperms in the perivitelline space and (c) irregular formation of the embryo may arise due to impaired formation of the HL. The decrease in fertilization success could be also related to the observed changes in intracellular pH at pCO2 * 1,000 latm or higher. Article in Journal/Newspaper Arctic Arctic Ocean acidification Spitsbergen Alfred Wegener Institute for Polar- and Marine Research (AWI): ePIC (electronic Publication Information Center) Arctic Helgoland Marine Research 68 2 341 356 |
institution |
Open Polar |
collection |
Alfred Wegener Institute for Polar- and Marine Research (AWI): ePIC (electronic Publication Information Center) |
op_collection_id |
ftawi |
language |
unknown |
description |
Fertilization depends on distribution and aggregation patterns of sea urchins which influence gamete contact time and may potentially enhance their vulnerability to ocean acidification. In this study, we conducted fertilization experiments to assess the effects of selected pH scenarios on fertilization success of Strongylocentrotus droebachiensis, from Spitsbergen, Arctic. Acidification was achieved by aerating seawater with different CO2 partial pressures to represent preindustrial and present conditions (measured*180–425 latm) and future acidification scenarios (*550–800, *1,300, *2,000 latm). Fertilization success was defined as the proportion of successful/unsuccessful fertilizations per treatment; eggs were classified according to features of their fertilization envelope (FE), hyaline layer (HL) and achievement of cellular division. The diagnostic findings of specific pathological aberrations were described in detail. We additionally measured intracellular pH changes in unfertilized eggs exposed for 1 h to selected acidification treatments using BCECF/AM. We conclude that (a) acidified conditions increase the proportion of eggs that failed fertilization, (b) acidification may increase the risk of polyspermy due to failures in the FE formation supported by the occasional observation ofmultiple sperms in the perivitelline space and (c) irregular formation of the embryo may arise due to impaired formation of the HL. The decrease in fertilization success could be also related to the observed changes in intracellular pH at pCO2 * 1,000 latm or higher. |
format |
Article in Journal/Newspaper |
author |
Bögner, Desislava Bickmeyer, Ulf Köhler, Angela |
spellingShingle |
Bögner, Desislava Bickmeyer, Ulf Köhler, Angela CO2-induced fertilization impairment in Strongylocentrotus droebachiensis collected in the Arctic |
author_facet |
Bögner, Desislava Bickmeyer, Ulf Köhler, Angela |
author_sort |
Bögner, Desislava |
title |
CO2-induced fertilization impairment in Strongylocentrotus droebachiensis collected in the Arctic |
title_short |
CO2-induced fertilization impairment in Strongylocentrotus droebachiensis collected in the Arctic |
title_full |
CO2-induced fertilization impairment in Strongylocentrotus droebachiensis collected in the Arctic |
title_fullStr |
CO2-induced fertilization impairment in Strongylocentrotus droebachiensis collected in the Arctic |
title_full_unstemmed |
CO2-induced fertilization impairment in Strongylocentrotus droebachiensis collected in the Arctic |
title_sort |
co2-induced fertilization impairment in strongylocentrotus droebachiensis collected in the arctic |
publisher |
SPRINGER |
publishDate |
2014 |
url |
https://epic.awi.de/id/eprint/35703/ https://epic.awi.de/id/eprint/35703/1/BoegnerBickmeyerKoehlerHMR2014.pdf https://hdl.handle.net/10013/epic.43637 https://hdl.handle.net/10013/epic.43637.d001 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Arctic Ocean acidification Spitsbergen |
genre_facet |
Arctic Arctic Ocean acidification Spitsbergen |
op_source |
EPIC3Helgoland Marine Research, SPRINGER, 68(2), pp. 341-356, ISSN: 1438-387X |
op_relation |
https://epic.awi.de/id/eprint/35703/1/BoegnerBickmeyerKoehlerHMR2014.pdf https://hdl.handle.net/10013/epic.43637.d001 Bögner, D. orcid:0000-0002-8318-8568 , Bickmeyer, U. orcid:0000-0002-5351-2902 and Köhler, A. (2014) CO2-induced fertilization impairment in Strongylocentrotus droebachiensis collected in the Arctic , Helgoland Marine Research, 68 (2), pp. 341-356 . doi:10.1007/s10152-014-0394-3 <https://doi.org/10.1007/s10152-014-0394-3> , hdl:10013/epic.43637 |
op_doi |
https://doi.org/10.1007/s10152-014-0394-3 |
container_title |
Helgoland Marine Research |
container_volume |
68 |
container_issue |
2 |
container_start_page |
341 |
op_container_end_page |
356 |
_version_ |
1798837918911954944 |