Microbial Functional Potential and Community Composition in Permafrost-Affected Soils of the NW Canadian Arctic
Permafrost-affected soils are among the most obvious ecosystems in which current microbial controls on organic matter decomposition are changing as a result of global warming. Warmer conditions in polygonal tundra will lead to a deepening of the seasonal active layer, provoking changes in microbial...
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ftawi:oai:epic.awi.de:35398 2023-05-15T14:27:25+02:00 Microbial Functional Potential and Community Composition in Permafrost-Affected Soils of the NW Canadian Arctic Frank-Fahle, Béatrice A. Yergeau, Etienne Greer, Charles W. Lantuit, Hugues Wagner, Dirk 2014-01-08 application/pdf https://epic.awi.de/id/eprint/35398/ https://epic.awi.de/id/eprint/35398/1/Frank_fahle_2014.pdf http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0084761 https://hdl.handle.net/10013/epic.43370 https://hdl.handle.net/10013/epic.43370.d001 unknown PUBLIC LIBRARY SCIENCE https://epic.awi.de/id/eprint/35398/1/Frank_fahle_2014.pdf https://hdl.handle.net/10013/epic.43370.d001 Frank-Fahle, B. A. , Yergeau, E. , Greer, C. W. , Lantuit, H. orcid:0000-0003-1497-6760 and Wagner, D. (2014) Microbial Functional Potential and Community Composition in Permafrost-Affected Soils of the NW Canadian Arctic , PLoS ONE, 9 (1), e84761 . doi:10.1371/journal.pone.0084761 <https://doi.org/10.1371/journal.pone.0084761> , hdl:10013/epic.43370 EPIC3PLoS ONE, PUBLIC LIBRARY SCIENCE, 9(1), pp. e84761, ISSN: 1932-6203 Article NonPeerReviewed 2014 ftawi https://doi.org/10.1371/journal.pone.0084761 2022-07-10T23:12:19Z Permafrost-affected soils are among the most obvious ecosystems in which current microbial controls on organic matter decomposition are changing as a result of global warming. Warmer conditions in polygonal tundra will lead to a deepening of the seasonal active layer, provoking changes in microbial processes and possibly resulting in exacerbated carbon degradation under increasing anoxic conditions. To identify current microbial assemblages in carbon rich, water saturated permafrost environments, four polygonal tundra sites were investigated on Herschel Island and the Yukon Coast, Western Canadian Arctic. Ion Torrent sequencing of bacterial and archaeal 16S rRNA amplicons revealed the presence of all major microbial soil groups and indicated a local, vertical heterogeneity of the polygonal tundra soil community with increasing depth. Microbial diversity was found to be highest in the surface layers, decreasing towards the permafrost table. Quantitative PCR analysis of functional genes involved in carbon and nitrogen-cycling revealed a high functional potential in the surface layers, decreasing with increasing active layer depth. We observed that soil properties driving microbial diversity and functional potential varied in each study site. These results highlight the small-scale heterogeneity of geomorphologically comparable sites, greatly restricting generalizations about the fate of permafrost-affected environments in a warming Arctic. Article in Journal/Newspaper Arctic Arctic Global warming Herschel Herschel Island permafrost Tundra Yukon Alfred Wegener Institute for Polar- and Marine Research (AWI): ePIC (electronic Publication Information Center) Arctic Herschel Island ENVELOPE(-139.089,-139.089,69.583,69.583) Yukon PLoS ONE 9 1 e84761 |
institution |
Open Polar |
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Alfred Wegener Institute for Polar- and Marine Research (AWI): ePIC (electronic Publication Information Center) |
op_collection_id |
ftawi |
language |
unknown |
description |
Permafrost-affected soils are among the most obvious ecosystems in which current microbial controls on organic matter decomposition are changing as a result of global warming. Warmer conditions in polygonal tundra will lead to a deepening of the seasonal active layer, provoking changes in microbial processes and possibly resulting in exacerbated carbon degradation under increasing anoxic conditions. To identify current microbial assemblages in carbon rich, water saturated permafrost environments, four polygonal tundra sites were investigated on Herschel Island and the Yukon Coast, Western Canadian Arctic. Ion Torrent sequencing of bacterial and archaeal 16S rRNA amplicons revealed the presence of all major microbial soil groups and indicated a local, vertical heterogeneity of the polygonal tundra soil community with increasing depth. Microbial diversity was found to be highest in the surface layers, decreasing towards the permafrost table. Quantitative PCR analysis of functional genes involved in carbon and nitrogen-cycling revealed a high functional potential in the surface layers, decreasing with increasing active layer depth. We observed that soil properties driving microbial diversity and functional potential varied in each study site. These results highlight the small-scale heterogeneity of geomorphologically comparable sites, greatly restricting generalizations about the fate of permafrost-affected environments in a warming Arctic. |
format |
Article in Journal/Newspaper |
author |
Frank-Fahle, Béatrice A. Yergeau, Etienne Greer, Charles W. Lantuit, Hugues Wagner, Dirk |
spellingShingle |
Frank-Fahle, Béatrice A. Yergeau, Etienne Greer, Charles W. Lantuit, Hugues Wagner, Dirk Microbial Functional Potential and Community Composition in Permafrost-Affected Soils of the NW Canadian Arctic |
author_facet |
Frank-Fahle, Béatrice A. Yergeau, Etienne Greer, Charles W. Lantuit, Hugues Wagner, Dirk |
author_sort |
Frank-Fahle, Béatrice A. |
title |
Microbial Functional Potential and Community Composition in Permafrost-Affected Soils of the NW Canadian Arctic |
title_short |
Microbial Functional Potential and Community Composition in Permafrost-Affected Soils of the NW Canadian Arctic |
title_full |
Microbial Functional Potential and Community Composition in Permafrost-Affected Soils of the NW Canadian Arctic |
title_fullStr |
Microbial Functional Potential and Community Composition in Permafrost-Affected Soils of the NW Canadian Arctic |
title_full_unstemmed |
Microbial Functional Potential and Community Composition in Permafrost-Affected Soils of the NW Canadian Arctic |
title_sort |
microbial functional potential and community composition in permafrost-affected soils of the nw canadian arctic |
publisher |
PUBLIC LIBRARY SCIENCE |
publishDate |
2014 |
url |
https://epic.awi.de/id/eprint/35398/ https://epic.awi.de/id/eprint/35398/1/Frank_fahle_2014.pdf http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0084761 https://hdl.handle.net/10013/epic.43370 https://hdl.handle.net/10013/epic.43370.d001 |
long_lat |
ENVELOPE(-139.089,-139.089,69.583,69.583) |
geographic |
Arctic Herschel Island Yukon |
geographic_facet |
Arctic Herschel Island Yukon |
genre |
Arctic Arctic Global warming Herschel Herschel Island permafrost Tundra Yukon |
genre_facet |
Arctic Arctic Global warming Herschel Herschel Island permafrost Tundra Yukon |
op_source |
EPIC3PLoS ONE, PUBLIC LIBRARY SCIENCE, 9(1), pp. e84761, ISSN: 1932-6203 |
op_relation |
https://epic.awi.de/id/eprint/35398/1/Frank_fahle_2014.pdf https://hdl.handle.net/10013/epic.43370.d001 Frank-Fahle, B. A. , Yergeau, E. , Greer, C. W. , Lantuit, H. orcid:0000-0003-1497-6760 and Wagner, D. (2014) Microbial Functional Potential and Community Composition in Permafrost-Affected Soils of the NW Canadian Arctic , PLoS ONE, 9 (1), e84761 . doi:10.1371/journal.pone.0084761 <https://doi.org/10.1371/journal.pone.0084761> , hdl:10013/epic.43370 |
op_doi |
https://doi.org/10.1371/journal.pone.0084761 |
container_title |
PLoS ONE |
container_volume |
9 |
container_issue |
1 |
container_start_page |
e84761 |
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1766301153349337088 |