Molecular analyses of gut contents: elucidating the feeding of co-occurring salps in the Lazarev Sea from a different perspective
The diet of Antarctic salps was elucidated by investigating their gut content using “Automated Ribosomal Intergenic Spacer Analysis” and 454-pyrosequencing. Salp samples were collected during the Lazarev Krill Study in the Western Weddell Sea (summer 2005/06 and 2007/08, fall 2004, and winter 2006)....
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CAMBRIDGE UNIV PRESS
2014
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ftawi:oai:epic.awi.de:35075 2023-05-15T13:40:26+02:00 Molecular analyses of gut contents: elucidating the feeding of co-occurring salps in the Lazarev Sea from a different perspective Metfies, Katja Nicolaus, Anja Harbou, Lena von Bathmann, Ulrich Peeken, Ilka 2014-10 application/pdf https://epic.awi.de/id/eprint/35075/ https://epic.awi.de/id/eprint/35075/2/Metfies2014.pdf https://hdl.handle.net/10013/epic.44051 https://hdl.handle.net/10013/epic.44051.d002 unknown CAMBRIDGE UNIV PRESS https://epic.awi.de/id/eprint/35075/2/Metfies2014.pdf https://hdl.handle.net/10013/epic.44051.d002 Metfies, K. orcid:0000-0003-3073-8033 , Nicolaus, A. orcid:0000-0002-2026-3540 , Harbou, L. v. , Bathmann, U. and Peeken, I. orcid:0000-0003-1531-1664 (2014) Molecular analyses of gut contents: elucidating the feeding of co-occurring salps in the Lazarev Sea from a different perspective , Antarctic Science, 26 (5), pp. 545-553 . doi:10.1017/S0954102014000157 <https://doi.org/10.1017/S0954102014000157> , hdl:10013/epic.44051 EPIC3Antarctic Science, CAMBRIDGE UNIV PRESS, 26(5), pp. 545-553, ISSN: 0954-1020 Article isiRev 2014 ftawi https://doi.org/10.1017/S0954102014000157 2021-12-24T15:39:24Z The diet of Antarctic salps was elucidated by investigating their gut content using “Automated Ribosomal Intergenic Spacer Analysis” and 454-pyrosequencing. Salp samples were collected during the Lazarev Krill Study in the Western Weddell Sea (summer 2005/06 and 2007/08, fall 2004, and winter 2006). Two salp species, Salpa thompsoni and Ihlea racovitzai, both occur in the Southern Ocean, can overlap geographically and seasonally. Here we provide evidence that despite the non-selective feeding mechanism, the two co-occuring salp species might have different niches within one given habitat. ARISA-patterns of 93 salp gut content samples revealed strong differences between the two salp species, even at the same sampling site. These differences were confirmed by 454-pyrosequencing of the V4-18S rDNA of ten salps. The pyrosequencing data indicate that flagellates, in particular dinophyceae constitute a high proportion of the sequence reads identified in the gut content of both salp species. However, within the dinophyceae differences in the read composition were detected between the two salp species. This supports the findings of a previous study where fatty acid signatures indicate a flagellate based diet of salps, even though microscopic analyses identified diatoms as the dominant component of salp gut contents. Article in Journal/Newspaper Antarc* Antarctic Antarctic Science Lazarev Sea Southern Ocean Weddell Sea Alfred Wegener Institute for Polar- and Marine Research (AWI): ePIC (electronic Publication Information Center) Antarctic Lazarev ENVELOPE(12.917,12.917,-69.967,-69.967) Lazarev Sea ENVELOPE(7.000,7.000,-68.000,-68.000) Southern Ocean Weddell Weddell Sea Antarctic Science 26 5 545 553 |
institution |
Open Polar |
collection |
Alfred Wegener Institute for Polar- and Marine Research (AWI): ePIC (electronic Publication Information Center) |
op_collection_id |
ftawi |
language |
unknown |
description |
The diet of Antarctic salps was elucidated by investigating their gut content using “Automated Ribosomal Intergenic Spacer Analysis” and 454-pyrosequencing. Salp samples were collected during the Lazarev Krill Study in the Western Weddell Sea (summer 2005/06 and 2007/08, fall 2004, and winter 2006). Two salp species, Salpa thompsoni and Ihlea racovitzai, both occur in the Southern Ocean, can overlap geographically and seasonally. Here we provide evidence that despite the non-selective feeding mechanism, the two co-occuring salp species might have different niches within one given habitat. ARISA-patterns of 93 salp gut content samples revealed strong differences between the two salp species, even at the same sampling site. These differences were confirmed by 454-pyrosequencing of the V4-18S rDNA of ten salps. The pyrosequencing data indicate that flagellates, in particular dinophyceae constitute a high proportion of the sequence reads identified in the gut content of both salp species. However, within the dinophyceae differences in the read composition were detected between the two salp species. This supports the findings of a previous study where fatty acid signatures indicate a flagellate based diet of salps, even though microscopic analyses identified diatoms as the dominant component of salp gut contents. |
format |
Article in Journal/Newspaper |
author |
Metfies, Katja Nicolaus, Anja Harbou, Lena von Bathmann, Ulrich Peeken, Ilka |
spellingShingle |
Metfies, Katja Nicolaus, Anja Harbou, Lena von Bathmann, Ulrich Peeken, Ilka Molecular analyses of gut contents: elucidating the feeding of co-occurring salps in the Lazarev Sea from a different perspective |
author_facet |
Metfies, Katja Nicolaus, Anja Harbou, Lena von Bathmann, Ulrich Peeken, Ilka |
author_sort |
Metfies, Katja |
title |
Molecular analyses of gut contents: elucidating the feeding of co-occurring salps in the Lazarev Sea from a different perspective |
title_short |
Molecular analyses of gut contents: elucidating the feeding of co-occurring salps in the Lazarev Sea from a different perspective |
title_full |
Molecular analyses of gut contents: elucidating the feeding of co-occurring salps in the Lazarev Sea from a different perspective |
title_fullStr |
Molecular analyses of gut contents: elucidating the feeding of co-occurring salps in the Lazarev Sea from a different perspective |
title_full_unstemmed |
Molecular analyses of gut contents: elucidating the feeding of co-occurring salps in the Lazarev Sea from a different perspective |
title_sort |
molecular analyses of gut contents: elucidating the feeding of co-occurring salps in the lazarev sea from a different perspective |
publisher |
CAMBRIDGE UNIV PRESS |
publishDate |
2014 |
url |
https://epic.awi.de/id/eprint/35075/ https://epic.awi.de/id/eprint/35075/2/Metfies2014.pdf https://hdl.handle.net/10013/epic.44051 https://hdl.handle.net/10013/epic.44051.d002 |
long_lat |
ENVELOPE(12.917,12.917,-69.967,-69.967) ENVELOPE(7.000,7.000,-68.000,-68.000) |
geographic |
Antarctic Lazarev Lazarev Sea Southern Ocean Weddell Weddell Sea |
geographic_facet |
Antarctic Lazarev Lazarev Sea Southern Ocean Weddell Weddell Sea |
genre |
Antarc* Antarctic Antarctic Science Lazarev Sea Southern Ocean Weddell Sea |
genre_facet |
Antarc* Antarctic Antarctic Science Lazarev Sea Southern Ocean Weddell Sea |
op_source |
EPIC3Antarctic Science, CAMBRIDGE UNIV PRESS, 26(5), pp. 545-553, ISSN: 0954-1020 |
op_relation |
https://epic.awi.de/id/eprint/35075/2/Metfies2014.pdf https://hdl.handle.net/10013/epic.44051.d002 Metfies, K. orcid:0000-0003-3073-8033 , Nicolaus, A. orcid:0000-0002-2026-3540 , Harbou, L. v. , Bathmann, U. and Peeken, I. orcid:0000-0003-1531-1664 (2014) Molecular analyses of gut contents: elucidating the feeding of co-occurring salps in the Lazarev Sea from a different perspective , Antarctic Science, 26 (5), pp. 545-553 . doi:10.1017/S0954102014000157 <https://doi.org/10.1017/S0954102014000157> , hdl:10013/epic.44051 |
op_doi |
https://doi.org/10.1017/S0954102014000157 |
container_title |
Antarctic Science |
container_volume |
26 |
container_issue |
5 |
container_start_page |
545 |
op_container_end_page |
553 |
_version_ |
1766133894475677696 |