Strong shift from HCO3- to CO2 uptake in Emiliania huxleyi with acidification: new approach unravels acclimation versus short-term pH effects
Effects of ocean acidification on Emiliania huxleyi strain RCC 1216 (calcifying, diploid life-cycle stage) and RCC 1217 (non-calcifying, haploid life-cycle stage) were investigated by measuring growth, elemental composition, and production rates under different pCO2 levels (380 and 950 μatm). In the...
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Online Access: | https://epic.awi.de/id/eprint/35021/ https://epic.awi.de/id/eprint/35021/1/Kottmeier_et_al_2014.pdf https://hdl.handle.net/10013/epic.43140 https://hdl.handle.net/10013/epic.43140.d001 |
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ftawi:oai:epic.awi.de:35021 2023-05-15T17:51:38+02:00 Strong shift from HCO3- to CO2 uptake in Emiliania huxleyi with acidification: new approach unravels acclimation versus short-term pH effects Kottmeier, Dorothee Rokitta, Sebastian Tortell, Philippe D. Rost, Bjoern 2014-02 application/pdf https://epic.awi.de/id/eprint/35021/ https://epic.awi.de/id/eprint/35021/1/Kottmeier_et_al_2014.pdf https://hdl.handle.net/10013/epic.43140 https://hdl.handle.net/10013/epic.43140.d001 unknown SPRINGER https://epic.awi.de/id/eprint/35021/1/Kottmeier_et_al_2014.pdf https://hdl.handle.net/10013/epic.43140.d001 Kottmeier, D. , Rokitta, S. orcid:0000-0002-7540-9033 , Tortell, P. D. and Rost, B. orcid:0000-0001-5452-5505 (2014) Strong shift from HCO3- to CO2 uptake in Emiliania huxleyi with acidification: new approach unravels acclimation versus short-term pH effects , Photosynthesis Research, 121 , pp. 265-275 . doi:10.1007/s11120-014-9984-9 <https://doi.org/10.1007/s11120-014-9984-9> , hdl:10013/epic.43140 EPIC3Photosynthesis Research, SPRINGER, 121, pp. 265-275, ISSN: 0166-8595 Article isiRev info:eu-repo/semantics/article 2014 ftawi https://doi.org/10.1007/s11120-014-9984-9 2021-12-24T15:39:21Z Effects of ocean acidification on Emiliania huxleyi strain RCC 1216 (calcifying, diploid life-cycle stage) and RCC 1217 (non-calcifying, haploid life-cycle stage) were investigated by measuring growth, elemental composition, and production rates under different pCO2 levels (380 and 950 μatm). In these differently acclimated cells, the photosynthetic carbon source was assessed by a 14C disequilibrium assay, conducted over a range of ecologically relevant pH values (7.9–8.7). In agreement with previous studies, we observed decreased calcification and stimulated biomass production in diploid cells under high pCO2, but no CO2-dependent changes in biomass production for haploid cells. In both life-cycle stages, the relative contributions of CO2 and HCO3 − uptake depended strongly on the assay pH. At pH values ≤ 8.1, cells preferentially used CO2 (≥ 90 % CO2), whereas at pH values ≥ 8.3, cells progressively increased the fraction of HCO3 − uptake (~45 % CO2 at pH 8.7 in diploid cells; ~55 % CO2 at pH 8.5 in haploid cells). In contrast to the short-term effect of the assay pH, the pCO2 acclimation history had no significant effect on the carbon uptake behavior. A numerical sensitivity study confirmed that the pH-modification in the 14C disequilibrium method yields reliable results, provided that model parameters (e.g., pH, temperature) are kept within typical measurement uncertainties. Our results demonstrate a high plasticity of E. huxleyi to rapidly adjust carbon acquisition to the external carbon supply and/or pH, and provide an explanation for the paradoxical observation of high CO2 sensitivity despite the apparently high HCO3 − usage seen in previous studies. Article in Journal/Newspaper Ocean acidification Alfred Wegener Institute for Polar- and Marine Research (AWI): ePIC (electronic Publication Information Center) Photosynthesis Research 121 2-3 265 275 |
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Open Polar |
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Alfred Wegener Institute for Polar- and Marine Research (AWI): ePIC (electronic Publication Information Center) |
op_collection_id |
ftawi |
language |
unknown |
description |
Effects of ocean acidification on Emiliania huxleyi strain RCC 1216 (calcifying, diploid life-cycle stage) and RCC 1217 (non-calcifying, haploid life-cycle stage) were investigated by measuring growth, elemental composition, and production rates under different pCO2 levels (380 and 950 μatm). In these differently acclimated cells, the photosynthetic carbon source was assessed by a 14C disequilibrium assay, conducted over a range of ecologically relevant pH values (7.9–8.7). In agreement with previous studies, we observed decreased calcification and stimulated biomass production in diploid cells under high pCO2, but no CO2-dependent changes in biomass production for haploid cells. In both life-cycle stages, the relative contributions of CO2 and HCO3 − uptake depended strongly on the assay pH. At pH values ≤ 8.1, cells preferentially used CO2 (≥ 90 % CO2), whereas at pH values ≥ 8.3, cells progressively increased the fraction of HCO3 − uptake (~45 % CO2 at pH 8.7 in diploid cells; ~55 % CO2 at pH 8.5 in haploid cells). In contrast to the short-term effect of the assay pH, the pCO2 acclimation history had no significant effect on the carbon uptake behavior. A numerical sensitivity study confirmed that the pH-modification in the 14C disequilibrium method yields reliable results, provided that model parameters (e.g., pH, temperature) are kept within typical measurement uncertainties. Our results demonstrate a high plasticity of E. huxleyi to rapidly adjust carbon acquisition to the external carbon supply and/or pH, and provide an explanation for the paradoxical observation of high CO2 sensitivity despite the apparently high HCO3 − usage seen in previous studies. |
format |
Article in Journal/Newspaper |
author |
Kottmeier, Dorothee Rokitta, Sebastian Tortell, Philippe D. Rost, Bjoern |
spellingShingle |
Kottmeier, Dorothee Rokitta, Sebastian Tortell, Philippe D. Rost, Bjoern Strong shift from HCO3- to CO2 uptake in Emiliania huxleyi with acidification: new approach unravels acclimation versus short-term pH effects |
author_facet |
Kottmeier, Dorothee Rokitta, Sebastian Tortell, Philippe D. Rost, Bjoern |
author_sort |
Kottmeier, Dorothee |
title |
Strong shift from HCO3- to CO2 uptake in Emiliania huxleyi with acidification: new approach unravels acclimation versus short-term pH effects |
title_short |
Strong shift from HCO3- to CO2 uptake in Emiliania huxleyi with acidification: new approach unravels acclimation versus short-term pH effects |
title_full |
Strong shift from HCO3- to CO2 uptake in Emiliania huxleyi with acidification: new approach unravels acclimation versus short-term pH effects |
title_fullStr |
Strong shift from HCO3- to CO2 uptake in Emiliania huxleyi with acidification: new approach unravels acclimation versus short-term pH effects |
title_full_unstemmed |
Strong shift from HCO3- to CO2 uptake in Emiliania huxleyi with acidification: new approach unravels acclimation versus short-term pH effects |
title_sort |
strong shift from hco3- to co2 uptake in emiliania huxleyi with acidification: new approach unravels acclimation versus short-term ph effects |
publisher |
SPRINGER |
publishDate |
2014 |
url |
https://epic.awi.de/id/eprint/35021/ https://epic.awi.de/id/eprint/35021/1/Kottmeier_et_al_2014.pdf https://hdl.handle.net/10013/epic.43140 https://hdl.handle.net/10013/epic.43140.d001 |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_source |
EPIC3Photosynthesis Research, SPRINGER, 121, pp. 265-275, ISSN: 0166-8595 |
op_relation |
https://epic.awi.de/id/eprint/35021/1/Kottmeier_et_al_2014.pdf https://hdl.handle.net/10013/epic.43140.d001 Kottmeier, D. , Rokitta, S. orcid:0000-0002-7540-9033 , Tortell, P. D. and Rost, B. orcid:0000-0001-5452-5505 (2014) Strong shift from HCO3- to CO2 uptake in Emiliania huxleyi with acidification: new approach unravels acclimation versus short-term pH effects , Photosynthesis Research, 121 , pp. 265-275 . doi:10.1007/s11120-014-9984-9 <https://doi.org/10.1007/s11120-014-9984-9> , hdl:10013/epic.43140 |
op_doi |
https://doi.org/10.1007/s11120-014-9984-9 |
container_title |
Photosynthesis Research |
container_volume |
121 |
container_issue |
2-3 |
container_start_page |
265 |
op_container_end_page |
275 |
_version_ |
1766158852109107200 |